Figure 2

Sterolgene v0 microarray design. Complete array containing 12 blocks was spotted twice to increase number of replicas. Each block contains positive and negative controls to enable spatial normalization. Each gene probe is spotted three times in two blocks. Green spots – positive controls: L – Firefly luciferase, A – ArrayControls Spikes (Ambion); white spots – negative controls: C – bacterial gene probe, SB – spotting buffer, N – empty spot; grey spots – salmon sperm DNA (S); yellow spots – gene probes (G).