Figure 1

Principal Component Analysis and distribution of normalised expression ratios for the total of genes analyzed. (A) PCA was performed to reduce the dimensions of expression data to 4 components. Components 1 to 4 explained respectively 43.84%, 11.24%, 10.58% and 8.54% of variation in the data. The first and second components were used as the X and Y axes to plot values from the 16 conditions in this study. Ag10, Ag20, Ag40, Ag80 and AgN represent the addition of Ag-NPs of 10-, 20-, 40- and 80-nm diameter or AgNO₃ bulk material, respectively. Ti10, Ti20, Ti40 and TiO the addition of TiO₂-NPs of 10-, 20-, 40-nm diameter and TiO₂ bulk material. CNT and CNT+ indicate COOH-MWCNTs added to non-supplemented or ABA-supplemented plants. (B) Box plot of the expression ratios for the 26,184 genes analysed with the AtV4 microarrays. Boxes represent 10th and 90th percentiles, with the median (Q₂) in the middle line of the box. Minimum and maximum values (snapped to Q₁-1.5 x IQR and Q₃ + 1.5 x IQR, IQR = Interquartile Range) are shown by the end of the vertical lines (whiskers) and upper or lower outliers by red diamonds.