Carotenoid biosynthetic genes in Brassica rapa: comparative genomic analysis, phylogenetic analysis, and expression profiling
- Peirong Li†1,
- Shujiang Zhang†1,
- Shifan Zhang1,
- Fei Li1,
- Hui Zhang1,
- Feng Cheng1,
- Jian Wu1,
- Xiaowu Wang1 and
- Rifei Sun1Email author
© Li et al. 2015
Received: 17 August 2014
Accepted: 22 May 2015
Published: 3 July 2015
Carotenoids are isoprenoid compounds synthesized by all photosynthetic organisms. Despite much research on carotenoid biosynthesis in the model plant Arabidopsis thaliana, there is a lack of information on the carotenoid pathway in Brassica rapa. To better understand its carotenoid biosynthetic pathway, we performed a systematic analysis of carotenoid biosynthetic genes at the genome level in B. rapa.
We identified 67 carotenoid biosynthetic genes in B. rapa, which were orthologs of the 47 carotenoid genes in A. thaliana. A high level of synteny was observed for carotenoid biosynthetic genes between A. thaliana and B. rapa. Out of 47 carotenoid biosynthetic genes in A. thaliana, 46 were successfully mapped to the 10 B. rapa chromosomes, and most of the genes retained more than one copy in B. rapa. The gene expansion was caused by the whole-genome triplication (WGT) event experienced by Brassica species. An expression analysis of the carotenoid biosynthetic genes suggested that their expression levels differed in root, stem, leaf, flower, callus, and silique tissues. Additionally, the paralogs of each carotenoid biosynthetic gene, which were generated from the WGT in B. rapa, showed significantly different expression levels among tissues, suggesting differentiated functions for these multi-copy genes in the carotenoid pathway.
This first systematic study of carotenoid biosynthetic genes in B. rapa provides insights into the carotenoid metabolic mechanisms of Brassica crops. In addition, a better understanding of carotenoid biosynthetic genes in B. rapa will contribute to the development of conventional and transgenic B. rapa cultivars with enriched carotenoid levels in the future.
KeywordsBiosynthetic pathway Carotenoid biosynthetic genes Comparative genomics Expression analysis Brassica rapa
Carotenoids represent a group of natural pigments derived from the general isoprenoid biosynthetic pathway. The reaction fundamentals and enzymes involved in the carotenoid pathway have been well studied in Arabidopsis thaliana . Carotenoids are mainly synthesized from isopentenyl diphosphate (IPP) and dimethylallyl diphosphate produced by the 2-C-methyl-D-erythritol-4-phosphate (MEP) pathway. The enzymes from the upstream MEP pathway include 1-deoxy-D-xylulose-5-phosphate synthase (DXS), 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR), 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (CMK), 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (MDS), 4-hydroxy-3-methylbut-2-enyl diphosphate synthase (HDS), and 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (HDR).
Additionally, isopentenyl pyrophosphate isomerase (IPPI) catalyzes the isomerization of dimethylallyl diphosphate to IPP, while geranyl pyrophosphate synthase (GPS) transforms IPP to geranyl pyrophosphate (GPP) [2–12]. The first key step for carotenoid biosynthesis is the production of a 40-carbon phytoene from paired geranylgeranyl pyrophosphate (GGPP) molecules. The process is catalyzed by enzyme phytoene synthase (PSY) [13, 14]. Then, a series of desaturases and isomerases participate in the generation of lycopene (colored) from phytoene (no color), including phytoene desaturase (PDS), ζ-carotene desaturase (ZDS), 15-cis-ζ-carotene isomerase (Z-ISO), and carotenoid isomerase (CRTISO) [15–18]. Lycopene represents the first branch point of the carotenoid pathway and is catalyzed by two competing enzymes, lycopene β-cyclase and lycopene ε-cyclase, which result in the production of β-carotene and α-carotene, respectively . Then, carotenoid cleavage dioxygenases (CCD) participate in the formation of apocarotenoid . Cyclic carotenes are further modified by two different types of carotenoid hydroxylases in A. thaliana: non-heme di-iron enzymes (BCH type) and cytochrome P450 enzymes (CYP97 type), which include BCH1, BCH2, LUT5, LUT1, and CYP97B3 [21–23]. Zeaxanthin then enters the xanthophyll cycle through the stepwise activities of zeaxanthin epoxidase (ZEP) and violaxanthin de-epoxidase (VDE) [24, 25]. The pigments antheraxanthin and violaxanthin produced by the above processes are further converted to neoxanthin by neoxanthin synthase (NSY) . Finally, the enzymes 9-cis-epoxycarotenoid dioxygenase (NCED), xanthoxin dehydrogenase (ABA2), and abscisic-aldehyde oxidase (AAO3) catalyze violaxanthin and neoxanthin to produce xanthoxin and abscisic acid, respectively [26–31].
The genus Brassica includes many vegetable crops, such as B. rapa, Brassica oleracea, Brassica napus, Brassica parachinensis, and Brassica juncea. B. rapa L. ssp. pekinensis (Chinese Cabbage) evolved in China and is an important vegetable crop in Asia. The inner leaves of several cultivars of heading B. rapa are orange and yellow, and are abundant in lutein, β-carotene, and prolycopene [32–34]. Because of the previous absence of genome information, little is known about the genes involved in the carotenoid biosynthetic pathway of B. rapa [35, 36]. The sequencing and release of the B. rapa genome , provides a good opportunity to systematically study the carotenoid biosynthetic genes in B. rapa. A complete understanding of the carotenogenesis genes is important for elucidating the mechanisms of carotenoid biosynthesis in B. rapa, as well as for the breeding of new B. rapa varieties with rich carotenoids, which are good for human health.
Whole-genome duplication events provide abundant amounts of genetic material for gene family expansion or the evolution of new genes in plants [38, 39]. B. rapa experienced a whole-genome triplication (WGT) event after its divergence from A. thaliana and has three subgenomes in its nucleus . The level of gene loss among the three subgenomes of B. rapa is biased: fragments with the highest gene densities comprise subgenome LF, fragments with moderate gene densities comprise subgenome MF1, and those with the least genes comprise subgenome MF2 [41, 42]. The fragments of the three subgenomes in B. rapa have been well distinguished; therefore, differences in gene loss among these subgenomes can be identified unambiguously. Although the influence of WGT on some gene families has been studied [43, 44], there is presently no detailed information on the evolution of carotenoid biosynthetic genes after the WGT in B. rapa. To obtain comprehensive information on the carotenoid biosynthetic pathway in B. rapa and to explore the effect of the WGT on these genes, we performed a comparative genomic analysis between B. rapa and A. thaliana using the genome sequences and annotation information of the two species . We investigated the evolution and functions of carotenoid biosynthetic genes in B. rapa by constructing phylogenic trees and analyzing their transcription patterns. The systematic analysis of carotenoid biosynthesis genes in B. rapa will improve our understanding of the genetic mechanisms of carotenoid biosynthesis and carotenoid accumulation in B. rapa crops.
Results and discussion
Identification of carotenoid biosynthetic genes in B. rapa
Carotenoid biosynthetic genes identified in Brassica rapa
MEP pathway to GGPP genes
Carotenoid biosynthetic genes
Chromosomal and subgenomic distributions
The genome structure of B. rapa was shaped by the WGT event followed by extensive diploidization . In the genome, fragments with the highest gene densities are in subgenome LF, fragments with moderate gene densities are in subgenome MF1, and the fragments with the least genes are in subgenome MF2 [37, 42]. With this subgenomic information, we then assigned all B. rapa carotenoid biosynthesis genes to the three subgenomes. There were 28, 23, and 16 genes located in LF, MF1, and MF2, respectively. There were more genes located in LF, while fewer genes were distributed in MF1 and even fewer genes in MF2. Of the 64 syntenic orthologs, 27 were in LF, 22 were in MF1, and 15 were in MF2. The proportion of total subgenomic B. rapa genes was used as the background to calculate the P-value using Fisher’s t-test. The P-value was 0.715 (>0.05), indicating that the proportion of carotenoid biosynthetic genes in each subgenome of B. rapa was not significantly different from the background. These results show that the distribution of carotenoid biosynthetic genes is consistent with the gene fractionation status at the whole-genome level [37, 42]. Based on the determination of these carotenoid biosynthetic genes, the carotenoid biosynthetic pathway in B. rapa was thus established.
Evolution of the PSY genes in B. rapa
PSY is a rate-limiting enzyme in Solanum lycopersicum fruit, B. napus seeds, Gentiana scabra Bunge flowers, and other plants [47–50], and is a key committed enzyme in the carotenoid biosynthetic pathway . In A. thaliana, a single PSY gene regulates phytoene synthesis in all tissues , while three genes encoding PSY enzymes were retained in B. rapa and B. oleracea after the WGT event. The PSY gene family expansion preceded the speciation of B. rapa and B. oleracea, dating back to the WGT event . Furthermore, six PSYs were retained in B. napus; however, we selected only three B. napus PSY genes that had complete sequences online. The evolution of carotenoid biosynthetic genes, such as PSYs, was consistent with the evolution of B. rapa.
Expression profiles of carotenoid biosynthetic genes in six organs of B. rapa
The B. rapa carotenoid biosynthetic genes could be divided into two clusters based on their expression patterns (Fig. 4; Additional file 2: Table S2). Cluster 1 was composed of genes that were expressed in all six organs, while Cluster 2 contained genes that had low expression levels and some organ-specific expression patterns. Cluster 1 included two expression groups. The first group showed a high expression level in all six organs. The second group exhibited a higher expression level in flowers, leaves, and siliques, indicating their roles in the carotenoid biosynthetic pathway in these tissues, than in roots, calli, and stems. The genes belonging to Cluster 2 had lower expression levels and could be subdivided into three groups. The first group was composed of 10 genes that had low expression levels in siliques, roots, calli, and stems. The genes in the second group exhibited low but stable expression levels in all six organs, except BrZEP2, which was highly expressed in flowers. This result was consistent with a previous study and indicates that BrZEP2 plays an important role in the synthesis and accumulation of carotenoids in B. rapa flowers . Most genes belonging to the third group exhibited low or undetectable expression levels in all six organs. Interestingly, the gene BrCCD8 was expressed in stems and roots but could not be detected in flowers, leaves, siliques, or calli, and BrNCED9.1 was highly expressed in siliques.
According to the expression analysis results, most B. rapa carotenoid biosynthetic genes appeared to have similar roles to their orthologs in other species. For example, it has been reported that the PSY, ZDS, PDS, and ZEP genes play crucial roles in carotenoid biosynthesis in A. thaliana . In B. rapa, these genes exhibited either predominant or specific expression patterns in leaves, such as BrZEP1, which was highly expressed in flowers and leaves. A. thaliana contains a family of 12 genes that are similar to GGPS, but only five GGPS genes have been shown to be expressed in different tissues during plant development . Although there are 13 duplicated BrGGPS genes in B. rapa, only BrGGPS1.1 was highly expressed in the six organs we examined. Moreover, most of the BrGGPS genes did not have detectable expression levels, which may be due to functional divergence after the triplication event or because they are specifically expressed at other developmental stages.
Expression variance among duplicated carotenoid biosynthetic genes in B. rapa
The expression differentiation between duplicate genes has been reported in many studies [59–62], and the functional divergence of PSY gene expression facilitates the accumulation of high levels of carotenoids in chromoplast-rich tissues in B. napus . In this paper, we studied the expression variance of genes that were created during a WGT event using RNA-Seq data. A gene expression analysis revealed an extensive variance between paralogs of each carotenoid biosynthetic gene. These duplicated gene expression variations may be signs of subfunctionalization among different tissues and contribute to an increased complexity in the regulatory networks of the carotenoid pathway after polyploidization.
We have identified 67 carotenoid biosynthetic genes in the genome of B. rapa through a comparative analysis between A. thaliana and B. rapa, and established the carotenoid biosynthetic pathway in B. rapa. Multiple copies of carotenoid biosynthetic genes were generated and retained after the WGT event and most of them maintained their syntenic relationships with their orthologs in A. thaliana. Fisher’s t-test indicated that the proportion of carotenoid biosynthetic genes in each subgenome of B. rapa was not significantly different from the whole genome backgrounds. The composition of carotenoid biosynthetic genes could explain the metabolic profiles of carotenoid accumulation and help to elaborate the genetic mechanism of carotenoid biosynthesis in B. rapa. The expression analysis of carotenoid biosynthetic genes showed that the paralogs of these genes were differentially expressed among roots, stems, leaves, flowers, calli, and siliques, suggesting that the functional differentiation of the duplicated carotenoid biosynthetic genes occurred after polyploidization.
Our study of the complete set of carotenoid biosynthetic genes in B. rapa will provide insights into carotenoid metabolic mechanisms in Brassica crops. In addition, a better understanding of carotenoid biosynthetic genes in B. rapa will facilitate the development of conventional and transgenic B. rapa cultivars with enriched carotenoid levels in the future.
Database for carotenoid biosynthetic gene identification in B. rapa
Gene sequences of A. thaliana involved in the carotenoid biosynthetic pathway were acquired from the KEGG pathway database (http://www.genome.jp/kegg/pathway.html) and TAIR database (www.arabidopsis.org). The B. rapa genome (version 1.5) and a set of annotated gene sequences from BRAD (http://Brassicadb.org) were used to identify the carotenoid biosynthetic genes in B. rapa .
Identification and analysis of orthologs between B. rapa and A. thaliana
The carotenoid biosynthetic gene and protein sequences of A. thaliana were aligned with the genome and protein sequences of B. rapa using BLASTN and BLASTP, respectively, with a cut off E-value ≤ 1E−10 and coverage ≥ 0.75. Syntenic orthologs between A. thaliana and B. rapa from BRAD (http://brassicadb.org/brad/) were identified based on sequence similarity (cutoff: E ≤ 10−20) and the collinearity of flanking genes .
The phylogenetic tree was constructed based on the full-length sequences of PSY proteins. A neighbor-joining tree was built using MEGA version 5.05 , adopting the Poisson correction distance. Support for the tree obtained was assessed using the bootstrap method with 1000 replicates.
Sequence data for the article can be found in the TAIR, NCBI, and Brassica (BRAD; http://brassicadb.org/brad/) databases. The accession numbers for the sequences are as follows: SlPSY1: NP_001234812.1; SlPSY2: NP_001234671.1; ZmPSY1: ACY70935.1; ZmPSY2: NP_001108117.1; ZmPSY3: NP_001108125.1; HcPSY: AEH05575.1; TaPSY: ABS83386.1; CaPSY: ADH04284.1; OsPSY1: AAS18307.1; OsPSY3: ABC75828.1; BnaC.PSY.a: JF920037; BnaA.PSY.b: JF920038; BnaA.PSY.c: JF920039, and all others can be found in Brassica databases.
Expression analysis of B. rapa carotenoid biosynthetic genes
The expression patterns of carotenoid biosynthetic genes in B. rapa were measured using RNA-Seq data . Six tissues, root, stem, leaf, flower, callus, and silique, of B. rapa accession Chiifu-401-42 were prepared for mRNA extraction. Plants were grown under greenhouse conditions at 22 °C. Callus tissue was obtained from tissue culture. Root, stem, and leaf tissues were collected from 7-week-old plants. Two samples of root and leaf tissues were generated from different batches of plants. Flower tissue was obtained from blooming plants on the same day and excluded the floral shoot. Silique tissue was collected from plants 15 days after pollination . FPKM values are available in Additional file 2: Table S2. Gene expression FPKM values were log2 transformed, and R software was employed to normalize the expression data and calculate hierarchical clustering .
Availability of supporting data
The B. rapa genome sequence (version 1.5) and gene sequences were acquired from BRAD (http://brassicadb.org/brad/).
We thank Dr. Shengyi Liu (Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences) for kindly providing the RNA-Seq data of B. rapa. This work was supported by the National Basic Research and Development Program (Chinese 973 Program, 2012CB113900) (to RS) and the National High Technology Research and Development Program of China (Chinese 863 Program, 2012AA100100) (to RS). Research was carried out in the Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, P.R. China.
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