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Table 1 Properties of RNA-seq libraries

From: Optimizing and benchmarking de novo transcriptome sequencing: from library preparation to assembly evaluation

Library Library preparation Sequencing
RNA source Duration of RNA fragmentation (min) × AMPure volume (targeted fraction to retain) PCR cycles
A -4 dpo whole embryoa 8 × 1.6 (>100 bp) 6 HiSeq 1/4 lanes, 171 cycles paired-end
B   2 × 0.7 (>300 bp) 6
C   6 MiSeq 1/4 lanes, 250 cycles paired-end
D 9 dpo whole embryo 8 × 1.6 (>100 bp) 2 HiSeq 1/4 lanes, 171 cycles paired-end
E 2 × 0.7 (>300 bp) 4
F 30 dpo head 4 × 1.0 (>150 bp) 6 HiSeq 2/3 lanes, 151 cycles paired-end
G 30 dpo liver    6  
H 30 dpo tail    6  
  1. aEmbryo of 4 days before the estimated day of oviposition