Fig. 1

Schematic of bioinformatics analysis. a TOMM biosynthesis begins with the ribosomal synthesis of a precursor peptide. The characteristic thiazoline/oxazoline heterocycles of a TOMM are installed by the C and D protein complex colored green and blue, respectively. Other tailoring enzymes (red and teal) often install additional modifications on the maturing product before the proteolytic cleavage (orange) of the leader peptide. b To identify TOMMs, all proteins containing a YcaO domain were identified using InterPro (IPR003776). The genomic regions surrounding the YcaO domains were retrieved, analyzed, and grouped by their cumulative BLAST bit score and synteny (Step 1). TOMM clusters were then separated from non-TOMM gene clusters determined by the inclusion of a C protein, precursor peptide, or bottromycin-like D protein (Step 2). (c) BLAST and synteny values from MultiGeneBlast were used to group TOMM clusters (Step 1). (d) A gene cluster was classified as a TOMM if it contained a C protein, precursor peptide, or was similar to bottromycin (Step 2)