Fig. 6

In situ localization of miR159, GAMYBL1, PMEI and cystatin. a and b localization of miR159 in WT and 7B-1 anthers, respectively. c and d GAMYBL1 in WT and 7B-1 anthers, respectively. e and f PMEI in WT anthers at late meiotic and binucleate microspores stages, respectively. g, h, and i PMEI in 7B-1 anthers at late meiotic stage, free binucleate microspores, and arrested binucleate microspores, respectively. j and k cystatin in WT and 7B-1 anthers, respectively. l and m cystatin in WT and 7B-1 anthers at binucleate microspores stage, respectively. n negative control, where a murine miR122a-specific probe was used. Arrows indicate the localization sites