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BMC Genomics

Open Access

Erratum to: The UBC-40 Urothelial Bladder Cancer Cell Line Index: a genomic resource for functional studies

  • Julie Earl1, 2,
  • Daniel Rico3,
  • Enrique Carrillo-de-Santa-Pau1,
  • Benjamín Rodríguez-Santiago4, 6,
  • Marinela Méndez-Pertuz1,
  • Herbert Auer7,
  • Gonzalo Gómez8,
  • H. Barton Grossman9,
  • David G. Pisano8,
  • Wolfgang A. Schulz10,
  • Luis A. Pérez-Jurado5, 6,
  • Alfredo Carrato2,
  • Dan Theodorescu11,
  • Stephen Chanock12,
  • Alfonso Valencia3 and
  • Francisco X. Real1, 5Email author
BMC Genomics201516:1019

https://doi.org/10.1186/s12864-015-2227-4

Received: 18 November 2015

Accepted: 18 November 2015

Published: 30 November 2015

The original article was published in BMC Genomics 2015 16:403

Keywords

Urothelial bladder cancerCell lineGenomicsMutationOncogeneTumor suppressor
Following the publication of our recent article in BMC Genomics [1] a number of aspects were called to our attention. We have carefully reviewed the experiments reported in this manuscript, as well as additional data from our laboratories, and would like to make the following points:
  1. 1.

    SW-850, included in our paper as a bladder cancer cell line, has been reported by several authors to be a pancreatic cancer cell line [25]. This is unlikely to be the case given that most pancreatic cancers are KRAS-mutant and both our analysis and a previous publication [5] indicate that the cells used are KRAS-wild type. However, given the controversy we recommend that these cells are not be used as bladder cancer models.

     
  2. 2.

    The Materials and Methods section of our paper indicated that the following cell lines were obtained from ATCC: 253 J, 575A, 639 V, JON, MGH-U4, SW-800, SW-1710, VM-CUB-2. However, these cultures have never been distributed by the ATCC. Therefore, they are available from us if other investigators are interested in using them.

     
  3. 3.

    It has been reported that UM-UC-2 is a T24 contaminant (http://www.ncbi.nlm.nih.gov/biosample/SAMN03151953 ,http://web.expasy.org/cellosaurus/CVCL_8155, http://iclac.org/wp-content/uploads/Cross-Contaminations-v7_2.pdf). We have used fingerprinting analysis to confirm this fact and the genetic identity of the cells/DNAs used in our experiments (Table 1).

     
  4. 4.

    It has been reported that VM-CUB-3 is a VM-CUB-1 contaminant (http://iclac.org/wp-content/uploads/Cross-Contaminations-v7_2.pdf, http://www.ncbi.nlm.nih.gov/biosample/3152040, http://web.expasy.org/cellosaurus/CVCL_9830). Nevertheless, our data indicate that the two cultures we used as VM-CUB-1 and VM-CUB-3 are distinct at the genomic level. Furthermore, as shown in Table 1, fingerprinting analysis clearly indicates that VM-CUB-1, VM-CUB-2, and VM-CUB-3 are different from each other. The origin of the DNA/cells in our paper was as indicated in the Material and Methods section and, therefore, investigators interested in these cells could directly address the corresponding co-authors.

     
Table 1

SNP fingerprint analysis of the bladder cancer cell lines suffering from an “identity crisis”

Cell Line

Comments

D5S818

D13S317

D7S820

D16S539

VWA

TH01

AM

TPOX

CSF1PO

VM- CUB-1 p29

 

11

10

8,11

11,12

18,19

9

X

8

11

VM-CUB-2 p112

 

11,13

12

8

9

14

7

X,Y

8,12

11,12

VM-CUB-3 p65

 

11

9,12

8,9

12

16

9.3

X

8

12

UM-UC-2 p264

DNA fingerprinting data, same as T24

10,12

10,12

10,11

9

17

6

X

8,11

10,12

T24 p8

ATCC

10,12

12

10,11

9

17,19

6

X

8,11

10,12

P passage

In the last few years there has been much emphasis on the need to accurately designate, identify, and characterize cancer cell lines as they are precious tools for cell biology studies [6, 7]. It is with this aim that we wish to make these comments and clarifications related to our recently published work.

Notes

Declarations

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Authors’ Affiliations

(1)
Epithelial Carcinogenesis Group, F BBVA Cancer Cell Biology Programme, CNIO (Spanish National Cancer Research Centre)
(2)
Servicio de Oncología Médica, Hospital Ramón y Cajal
(3)
Structural Computational Biology Group, Structural Biology and Biocomputing Programme, CNIO (Spanish National Cancer Research Centre)
(4)
Quantitative Genomic Medicine Laboratory, qGenomics
(5)
Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra
(6)
Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER)
(7)
Institut de Recerca Biomèdica de Barcelona, Parc Científic de Barcelona
(8)
Bioinformatics Unit, Structural Biology and Biocomputing Programme, CNIO (Spanish National Cancer Research Centre)
(9)
Department of Urology, MD Anderson Cancer Center
(10)
Department of Urology, Heinrich-Heine-University
(11)
University of Colorado Comprehensive Cancer Center
(12)
Translational Genomics Laboratory, Division of Cancer Epidemiology and Genetics, National Cancer Institute

References

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Copyright

© Earl et al. 2015

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