Erratum to: The UBC-40 Urothelial Bladder Cancer Cell Line Index: a genomic resource for functional studies
- Julie Earl1, 2,
- Daniel Rico3,
- Enrique Carrillo-de-Santa-Pau1,
- Benjamín Rodríguez-Santiago4, 6,
- Marinela Méndez-Pertuz1,
- Herbert Auer7,
- Gonzalo Gómez8,
- H. Barton Grossman9,
- David G. Pisano8,
- Wolfgang A. Schulz10,
- Luis A. Pérez-Jurado5, 6,
- Alfredo Carrato2,
- Dan Theodorescu11,
- Stephen Chanock12,
- Alfonso Valencia3 and
- Francisco X. Real1, 5Email author
© Earl et al. 2015
Received: 18 November 2015
Accepted: 18 November 2015
Published: 30 November 2015
The original article was published in BMC Genomics 2015 16:403
KeywordsUrothelial bladder cancer Cell line Genomics Mutation Oncogene Tumor suppressor
SW-850, included in our paper as a bladder cancer cell line, has been reported by several authors to be a pancreatic cancer cell line [2–5]. This is unlikely to be the case given that most pancreatic cancers are KRAS-mutant and both our analysis and a previous publication  indicate that the cells used are KRAS-wild type. However, given the controversy we recommend that these cells are not be used as bladder cancer models.
The Materials and Methods section of our paper indicated that the following cell lines were obtained from ATCC: 253 J, 575A, 639 V, JON, MGH-U4, SW-800, SW-1710, VM-CUB-2. However, these cultures have never been distributed by the ATCC. Therefore, they are available from us if other investigators are interested in using them.
It has been reported that UM-UC-2 is a T24 contaminant (http://www.ncbi.nlm.nih.gov/biosample/SAMN03151953 ,http://web.expasy.org/cellosaurus/CVCL_8155, http://iclac.org/wp-content/uploads/Cross-Contaminations-v7_2.pdf). We have used fingerprinting analysis to confirm this fact and the genetic identity of the cells/DNAs used in our experiments (Table 1).
It has been reported that VM-CUB-3 is a VM-CUB-1 contaminant (http://iclac.org/wp-content/uploads/Cross-Contaminations-v7_2.pdf, http://www.ncbi.nlm.nih.gov/biosample/3152040, http://web.expasy.org/cellosaurus/CVCL_9830). Nevertheless, our data indicate that the two cultures we used as VM-CUB-1 and VM-CUB-3 are distinct at the genomic level. Furthermore, as shown in Table 1, fingerprinting analysis clearly indicates that VM-CUB-1, VM-CUB-2, and VM-CUB-3 are different from each other. The origin of the DNA/cells in our paper was as indicated in the Material and Methods section and, therefore, investigators interested in these cells could directly address the corresponding co-authors.
SNP fingerprint analysis of the bladder cancer cell lines suffering from an “identity crisis”
VM- CUB-1 p29
DNA fingerprinting data, same as T24
In the last few years there has been much emphasis on the need to accurately designate, identify, and characterize cancer cell lines as they are precious tools for cell biology studies [6, 7]. It is with this aim that we wish to make these comments and clarifications related to our recently published work.
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