Fig. 5
Expression profiles of Cma_004516 in C. maxima. a, RT-PCR analysis of Cma_004516, Cma_004514 and Cma_004515 mRNA in various tissues. R, roots; V, vines; Tv, tip of the vines; L, leaves; Fb, flowers before anthesis; Fa, flowers after anthesis; Fr, young fruits; DAS, days after sowing. P1, maternal parent Rimu; P2, paternal parent SQ026; The right side shows the PCR amplification cycles. EF-1A was used as the internal control. b, Analysis of Cma_004516 gene expression in vines by real-time PCR. Each bar represents an average of three independent reactions, including both biological and technical replicates. Error bars indicated SD. c, Analysis of the activities of the Cma_004516 promoters by transient expression in tobacco leaves. a, promoter of the vine type; b, promoter of the bush type