Fig. 1
From: Modeling central metabolism and energy biosynthesis across microbial life
Core metabolic model construction pipeline. The pipeline starts with gene annotations provided by RAST annotation pipeline of assembled microbial genomes. Next, the CMMs are constructed based on a manually curated CMT that consists of biochemical reactions derived from phylogenetically diverse set of model organisms including Escherichia coli, Bacillus. subtilis, Pseudomonas aeroginosa, Clostridium acetobutylicum, and Paracococcus denitrificans. In the final step, FBA is performed optimizing the biomass or ATP hydrolysis as the objective function