Fig. 10

Cnidarian minicollagen gene tree. ML topology of minicollagen gene family in cnidarian taxa from NCBI Genbank and transcriptome components of A. alata in this study. Assumes the BLOSS62I-G-F model of amino acid evolution, as specified as most appropriate by ProtTest v. 3.2. Shimodaira-Hasegawa-like branch support indices are shown at each node. All A. alata minicollagen types were more closely related to non-cubozoan homologs. Following Shpirer et al. 2014, Group 1 minicollagens possess N- and C-terminal cysteine rich domains (CRDs) of a regular form (CXXXCXXXCXXXCXXXCC). We use the label Group 1 (variant) to identify those minicollagens having three or more regular CRDs. Group 2 minicollagens possess one regular and one irregular CRD, with the variant form having the regular CRD at the C-terminus, whereas Group 3 minicollagens possess irregular CRDs at both termini. One (comp76287_c0) of the three recovered minicollagen homologs from A. alata was expressed across all five samples, but most abundant in the tentacle (and pedalium base)