Fig. 4

qRT-PCR validation of differential gene expression. The left side indicates relative gene expression levels determined by qRT-PCR after rinsing 2, 7 days and chilling 4 weeks. The right side indicates RPKM levels calculated by the RSEM method. Error bars depict the standard error of the mean for three biological replicates. Coefficient analysis between gene expression ratios obtained from qRT-PCR and RNA-seq data. The correlation coefficient r values of the EID1, DREB2C, AFP2, AFP3, PP2CA and NCED1 were 0.998***, 0.978***, 0.982***, 0.956***, 0.986*** and 0.953***, respectively, whereas those of ABA-hy3, LEA D-34, GA2-ox8 and LeMADS ranged from 0.64 to 0.83 and were not significant (see Additional file 10)