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Table 1 Validation of RNA-sequencing data by qRT-PCR

From: Changes in Bacillus anthracis CodY regulation under host-specific environmental factor deprived conditions

Target gene Condition Fold-change in RNA-sequencinga Fold-change in qRT-PCR
BAS0563 34F2R 1 1
Cluster 6 34F2-Fe 14.17 402.52
  34F2-Bic ND 1.48
  34F2-Glu ND 1.33
  BCDR 6.17 6.69
  BCD-Fe ND 10.44
  BCD-Bic 23.50 1.975
  BCD-Glu ND 3.30
eno (BAS4985) 34F2R 1 1
Cluster 7 34F2-Fe 0.13 2.25
  34F2-Bic ND 7.70
  34F2-Glu 0.14 0.24
  BCDR 7.13 3.25
  BCD-Fe 0.97 5.57
  BCD-Bic 0.36 0.26
  BCD-Glu 0.08 0.37
tpiA (BAS4987) 34F2R 1 1
Cluster 1 34F2-Fe ND 0.52
  34F2-Bic 0.02 4.49
  34F2-Glu 0.36 0.04
  BCDR 9.85 1.95
  BCD-Fe 1.5 1.62
  BCD-Bic ND 0.07
  BCD-Glu ND 0.04
inhA1 (BAS1197) 34F2R 1 1
Cluster 14 34F2-Fe ND 0.87
  34F2-Bic ND 0.43
  34F2-Glu 0.25 2.66
  BCDR 10.61 37.71
  BCD-Fe 1.82 37.56
  BCD-Bic 0.43 4.74
  BCD-Glu 2.00 20.99
BAS4903 34F2R 1 1
Cluster 4 34F2-Fe ND 0.62
  34F2-Bic ND 0.38
  34F2-Glu 0.02 0.70
  BCDR 0.13 0.15
  BCD-Fe ND 0.12
  BCD-Bic 0.37 0.60
  BCD-Glu 0.94 0.99
BAS5069 34F2R 1 1
Cluster 11 34F2-Fe ND 0.55
  34F2-Bic 0.43 0.37
  34F2-Glu 0.36 0.75
  BCDR 0.14 0.10
  BCD-Fe 0.13 0.12
  BCD-Bic 3.01 0.90
  BCD-Glu ND 6.33
yceC (BAS0385) 34F2R 1 1
Cluster 1 34F2-Fe 1.35 8.13
  34F2-Bic ND 0.72
  34F2-Glu 1.62 0.22
  BCDR 13.06 5.68
  BCD-Fe 1.26 7.36
  BCD-Bic ND 0.27
  BCD-Glu ND 1.35
  1. aND, not detected in RNA-sequencing