Changes in Bacillus anthracis CodY regulation under host-specific environmental factor deprived conditions

BMC Genomics

Table 4 Primers used in this study

Oligonucleotide^a	Sequence^b (5’ → 3’)
Oligonucleotide^a	Forward	Reverse
500CodYUP	ATAGGATCCGAATTATTAGCAAAAAC	ATATCTAGATTAGTTTGTTTTTAATTTAGCA
500CodYDN	GGGCGGCCGCGTATCACGTGAAGG TGTGC	CACCTGCAGTGGAAACTAGGGCGA GTCAC
BAS0563	ACACCACAAGAGACATGTTCT	CGCATGTAATATTCTCGTACGTGT
yceC (BAS0385)	TCGATTTAACGAAAGGACAACCA	ACAACGGATGCATCACAATCT
inhA1 (BAS1197)	CATCAATCGCTTTGACAGCTGT	GCGCATCTGCTAAACGTTCT
BAS4903	GGCCGTATGCAAATGGTTCG	GCCCGAATTGCAATTGGTGT
eno (BAS4985)	TGTTTATGCTCGCGAAGTCCT	TGCTTCGTGCTCACCAGTAG
tpiA (BAS4987)	GCGTAAACCAATTATCGCAGGT	ACCAGGCGCTCTAAGAATAGA
BAS5069	TCGCAAGATTAGAGCATATGACA	GCCCTTGGCGGTCAAATAAC
BAS0036ChIP	ATTTTTAAATTGTAAGTGGAGATTGT	ACCATCTATTACTCATATTTCAAGAA
BAS1197ChIP	TTCAGAAAACATTGTTGAATTGTG	ATTTGTAAAAATTCCATAGTTAGCAT
BAS1350ChIP	AGCACAAATGATACTTGCAAA	TTAACGACATGTGTTGGACT
BAS3397ChIP	ATTTTTCGAGTTTTGTAAAGTGTT	TCAATGAGGTATAGTGTTTTAAAAGA
BAS3621ChIP	AGTTAATGAACATGCAATTTTCAT	GTAGTTTTATTTACTTAGAAAATTCAGAAT
BAS4069ChIP	ATCGCATCGTTTACTGTGTA	GGATTACTTCGTCCAATCGA
BAS4252ChIP	TAACTTTTTGGAATATTGGCACT	TTTTCATTGAAACTCCCCCT
BAS4963ChIP	AGCTCTCTGGAAAGAATGTG	CGTAAAAGCGTTGAAAAGGA

^aChIP, chromatin immunoprecipitation; denotes primers that were used in ChIP-qPCR
^bBoldface bases denote a restriction enzyme target site

ISSN: 1471-2164