Fig. 11

PCR amplification of genes from scaffolds 13 and 20 in isolates 14H1-11A and CIRAD86. PCR amplification was done for three genes encoding hypothetical proteins on scaffolds 13 and 20, as well as β-tubulin as a positive control. Genomic DNA from isolates 14H1-11A (used in this RNA-Seq analysis) and CIRAD86 (genome sequence publicly available) was used as a template for PCR assays, with water used as a negative control. Quick-Load 100 bp DNA ladder (NEB) was used as a molecular weight marker. Red rectangles mark the expected product size for each assay