Fig. 2

Quality control analyses of the metatranscriptome sequence. a Cluster dendrogram based on a Pearson distribution of all contigs following normalization. Samples are labelled with letters indicating their treatment (C = control, B = Beauveria) and colony number. b Plot of Biological coefficient of variation vs. average logCPM. Each spot represents an individual contig. c Multiple dimension scaling plot showing distances in gene expression profiles across biological replicates and treatment groups. d Results of a validation experiment showing the correlation between logCT from qPCR analyses (CT of treatment/CT of control) vs. logFC (counts per million of treatment/counts per million of control). Spearman’s correlation coefficient rho (ρ) reported shows a significant, strong negative correlation (p = 0.008). Each data point represents a single gene (n = 14; See Additional file 1: Table S2)