- Open Access
Erratum to: The UBC-40 Urothelial Bladder Cancer cell line index: a genomic resource for functional studies
- Julie Earl1, 2,
- Daniel Rico3,
- Enrique Carrillo-de-Santa-Pau1,
- Benjamín Rodríguez-Santiago4, 5, 6,
- Marinela Méndez-Pertuz1,
- Herbert Auer7,
- Gonzalo Gómez8,
- H. Barton Grossman9,
- David G. Pisano8,
- Wolfgang A. Schulz10,
- Luis A. Pérez-Jurado5, 6,
- Alfredo Carrato2,
- Dan Theodorescu11,
- Stephen Chanock12,
- Alfonso Valencia3 and
- Francisco X. Real1, 5Email author
© The Author(s). 2016
- Received: 25 September 2016
- Accepted: 20 October 2016
- Published: 25 October 2016
The original article was published in BMC Genomics 2015 16:403
Please see modification to the first erratum  below, in which the Grant support section should have been modified as well:
SW-850, included in our paper as a bladder cancer cell line, has been reported by several authors to be a pancreatic cancer cell line [2–5]. This is unlikely to be the case given that most pancreatic cancers are KRAS-mutant and both our analysis and a previous publication  indicate that the cells used are KRAS-wild type. However, given the controversy we recommend that these cells are not be used as bladder cancer models.
The Materials and Methods section of our paper indicated that the following cell lines were obtained from ATCC: 253 J, 575A, 639 V, JON, MGH-U4, SW-800, SW-1710, VM-CUB-2. However, these cultures have never been distributed by the ATCC. Therefore, they are available from us if other investigators are interested in using them.
- 3.It has been reported that UM-UC-2 is a T24 contaminant [6–8]. We have used fingerprinting analysis to confirm this fact and the genetic identity of the cells/DNAs used in our experiments (Table 1).Table 1
SNP fingerprint analysis of the bladder cancer cell lines suffering from an “identity crisis”
VM- CUB-1 p29
DNA fingerprinting data, same as T24
It has been reported that VM-CUB-3 is a VM-CUB-1 contaminant  [9, 10]. Nevertheless, our data indicate that the two cultures we used as VM-CUB-1 and VM-CUB-3 are distinct at the genomic level. Furthermore, as shown in Table 1, fingerprinting analysis clearly indicates that VM-CUB-1, VM-CUB-2, and VM-CUB-3 are different from each other. The origin of the DNA/cells in our paper was as indicated in the Material and Methods section and, therefore, investigators interested in these cells could directly address the corresponding co-authors.
In the last few years there has been much emphasis on the need to accurately designate, identify, and characterize cancer cell lines as they are precious tools for cell biology studies [11, 12]. It is with this aim that we wish to make these comments and clarifications related to our recently published work.
This work was supported, in part, by the Spanish Ministry of Economy and Competitivenes (MINECO) grants Consolider ONCOBIO CSD2007-00017, SAF2011-15934-E, the Institute of Health Carlos III through the Red Temática de Investigación Cooperativa en Cáncer (RTICC)] with reference number RD12/0036/0034, which is cofunded by European Regional Development Fund (ERDF); Asociación Española Contra el Cáncer, the project UROMOL EU-FP7-201663, and National Institutes of Health grants (NIH) grants RO1-CA089715; and CA075115 and CA104106 (to D.T.).
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Earl J, Rico D, Carrillo-de-Santa-Pau E, Rodríguez-Santiago B, Méndez M, Auer H, et al. Erratum to: The UBC-40 Urothelial Bladder Cancer Cell Line Index: a genomic resource for functional studies. BMC Genomics. 2015;16:1019.View ArticlePubMedPubMed CentralGoogle Scholar
- Earl J, Rico D, Carrillo-de-Santa-Pau E, Rodríguez-Santiago B, Méndez M, Auer H, et al. The UBC-40 Urothelial Bladder Cancer Cell Line Index: a genomic resource for functional studies. BMC Genomics. 2015;16:403.View ArticlePubMedPubMed CentralGoogle Scholar
- Fogh J, Fogh JM, Orfeo T. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J Natl Cancer Inst. 1977;59(1):221–6.PubMedGoogle Scholar
- Fogh J, Wright WC, Loveless JD. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J Natl Cancer Inst. 1977;58(2):209–14.PubMedGoogle Scholar
- Kalthoff H, Schmiegel W, Roeder C, Kasche D, Schmidt A, Lauer G, et al. p53 and K-RAS alterations in pancreatic epithelial cell lesions. Oncogene. 1993;8(2):289–98.PubMedGoogle Scholar
- Müller C, Bockhorn AG, Klusmeier S, Kiehl M, Roeder C, Kalthoff H, et al. Lovastatin inhibits proliferation of pancreatic cancer cell lines with mutant as well as with wild-type K-ras oncogene but has different effects on protein phosphorylation and induction of apoptosis. Int J Oncol. 1998;12(3):717–23.PubMedGoogle Scholar
- Freedman LP, Gibson MC, Ethier SP, Soule HR, Neve RM, Reid YA. Reproducibility: changing the policies and culture of cell line authentication. Nat Methods. 2015;12(6):493–7.View ArticlePubMedGoogle Scholar
- Yu M, Selvaraj SK, Liang-Chu MM, Aghajani S, Busse M, Yuan J, et al. A resource for cell line authentication, annotation and quality control. Nature. 2015;520(7547):307–11.View ArticlePubMedGoogle Scholar