Fig. 2

Overview of transcriptional changes. a Scree plot of eigenvalues for the dimensions extracted from a multidimensional scaling (MDS) analysis of the 500 genes with the highest variations in expression during LARC and SMRC development. Dimension 1 accounted for 45 % of the total variance, while dimension 2 accounted for 32 % of the total variance. b Projection of samples onto the first two MDS dimensions. Each sample, representing a single biological replicate of a single culture under one growth condition and one time point, is represented by a single symbol. Samples grown with trace carbon (leading to SMRCs) are colored red, while those with no carbon source (leading to LARCs) are depicted in blue. Different symbols indicate different time points as indicated in the legend at the bottom right. Points with same color and symbol represent the three biological replicates for the RNA-seq analyses. Arrows indicate the development of SMRCs and LARCs over time (0, 1, 3, 24 h and 14 d)