Fig. 2

Characterization of viral integration and viral-human fusion transcripts in AAE6 epithelia. a Integration site schematic showing viral and human junctions, including nucleotide positions, the early promotor, as well as viral-human fusion transcript between HPV16 early region and SLC26A2 exon 3. A coverage plot above the integrated HPV16 genome demonstrates coverage across the junction sites within SLC26A2 (5482 reads across a 4978 nt assembly containing the AAE6 integrated form flanked by 200 nt of SLC26A2), while a circular coverage plot on the right shows the full episomal assembly of the EPE6 episomal form. b Immunofluorescence overlays of EPE6 and AAE6 raft cultures (400× magnification). Nuclei are indicated by blue DAPI staining while SLC26A2 is indicated by red fluorescence