Fig. 6

Ion channel subtypes and families identified in C. borealis and H. americanus transcriptome assemblies. Amino acid sequence alignment was carried out using ClustalW, and subsequent trees were generated using ClustalW2_Phylogeny. We were not attempting to generate true phylogenetic relationships, but rather simply used tree-based analysis to identify putative gene families. Hence no bootstrap values are calculated. There was a 100 % overlap in identified genes from both species, with the exception of TRP channels. A comprehensive list of channel types identified, their putative membrane currents, and accession numbers are provided in Table 2. Transcripts with the prefix “Cb” were identified from C. borealis, while those with “Ha” were identified from H. americanus