Fig. 6

Comparison of Transcripts with Methanosarcina mazei [66] a) A comparison of mRNA half lives measured via our RNAseq data compared with a previous study using qtRT-PCR in the related organism Methanosarcina mazei growing in methanol or acetate. Cao et al. measured methyltransferase (mtaA1, mtaCB1) half-lives from methanol grown cells, while they measured acetoclastic gene (pta, ack) half-lives for acetate grown cells. As can be seen in the figure half lives match for methanol and acetate grown cells. Arrows indicate which bars correspond to the comparison to Cao et al. b) A comparison of mRNA copies per cell estimated via our RNAseq data, and previous studies that utilized RT-qPCR to quantify transcript abundance in the related organism Methanosarcina mazei grown in methanol (see Additional file 1: Figure S13 for acetate growth). Error bars are standard deviation of the mean for 3 replicates. Values from Cao et al. are for cells grown at 30 °C compared to our cells which were grown at 37 °C. All values agree within uncertanties except for cdh, mtaA2, and mtaB2 indicating the organisms have similar expression profiles and our estimates for mRNA counts are good