Marker development using SLAF-seq and whole-genome shotgun strategy to fine-map the semi-dwarf gene ari-e in barley

Table 4 PCR primers, product size and physical position designed from SLAF-seq strategy

Marker name	Forward sequence	Reverse sequence	Size	IBSC Physical Position	POPSEQ Phyiscal Position
DGSNP9	GGCATAACGGTAACCCAATG	TCAAATAGCCGTGTCCATGA	232 bp	Chr 5H 387,821,508	Chr 5H 451,386,904
DGSNP10	GTTGGCCCAAGATTTATCCC	CGACCCTGATCAAGAACACA	224 bp	Chr 5H 389,006,171	Chr 5H 487,359,370
DGSNP11	CTCATGACCAACGCTTTCAA	CTGTCGGTCAAGGACACAAA	132 bp	Chr 5H 389,903,941	Chr 5H 483,326,936
DGSNP15	ATTATGTGTTCCTGGGCCTG	CAGAAGGCAATGGATGATGA	113 bp	Chr 5H 393,057,839	Chr 5H 486,659,431
DGSNP21	ACATTCATGTTTCCCCGTGT	GGAAAGGACTAGTAGGGCCG	126 bp	Chr 5H 396,709,139	Chr 5H 490,942,368
DGSNP26	ACCCAATTCTCATAGGCACG	GAGAGGGGGAACACGTACAA	158 bp	Chr 5H 399,664,776	Chr 5H 480,758,192
DGSNP37	TCCCTTGCCAGAAAAACATC	TACGCAGCCTATACCATCCC	261 bp	Chr 5H 399,776,063	Chr 5H 494,670,447
DGSNP59	TTGGGATCTTTATGGCAAGC	TATTCCGGTACCTGCACCTC	238 bp	Chr 5H 400,302,818	Chr 5H 494,361,321
DGSNP76	CTTTCTTTGTAGTTGTACGCAT	TGCGTTAAAAGGCCTAAACA	144 bp	Chr 5H 400,112,902	Chr 5H 492,409,362

ISSN: 1471-2164