Computer aided identification of a Hevein-like antimicrobial peptide of bell pepper leaves for biotechnological use

Table 2 De novo sequencing of ions of the P4-MEC fraction after controlled trypsinolysis^a

Ion (m/z)^b	Sequence	Modifications^c
1593	36 LCCSQFGFCGTTR 48	Alkylated Cys
1502	31 VCANRLCCSQFGFCGTTR 48	Alkylated Cys and R48 partially degraded
1290	49 EYCGAGCQSNCR 60	Not-alkylated Cys
1461	49 EYCGAGCQSNCR 60	Alkylated Cys

^aTrypsinolysis was developed by 6, 12, 24, and 36 h in P4-MEC fraction and the ions were analyzed by mass spectrometry
^bAll ions were detected in all MS2 spectra for all trypsinolysis times
^cThe mass-value of 57.05 Da is added in the alkylation event for each sulfhydryl group of the cysteine residue

ISSN: 1471-2164