Table 7 Primers used for genes cloning and quantitative real-time PCR
Primer name | Sequence (5′-3′) | Items |
|---|---|---|
3’-RACE outer | R:CTGATCTAGAGGTACCGGATCC | 3’-RACE |
Clock 3’-RACE inner | F:AGACGGCTGTAGTGGCTC | 3’-RACE |
Clock 3’-RACE inner | F:AGACGGCTGTAGTGGCTC | 3’-RACE |
5’-RACE outer | F:GACTCGAGTCGACATCG | 5’-RACE |
Cry1 5’-RACE inner | R: TTGGCATTCATTCTGGGACG | 5’-RACE |
Cry1 5’-RACE inner | R: GGTCTGGAACCGTTTGTAGG | 5’-RACE |
Cry1 3’-RACE inner | F: TCAAGGAGACTGGCAAAGCG | 3’-RACE |
Cry1 3’-RACE inner | F: CCACAAGCCAGCATCAGCAC | 3’-RACE |
Per1 5’-RACE inner | R: ATTTAGAGTGCTGGCGTGGC | 5’-RACE |
Per1 5’-RACE inner | R: GGGCGAACCTTCAGATCCTG | 5’-RACE |
Per1 3’-RACE inner | F: GAGAACGGTGAAACAAATGA | 3’-RACE |
Per1 3’-RACE inner | F: ACGCTTCACCGAGGAACAGA | 3’-RACE |
Nr1d2 5’-RACE inner | R: CAGTCTGAAGGGGCAGTGGT | 5’-RACE |
Nr1d2 5’-RACE inner | R: GACTGGTAGCTGCCGTTGGA | 5’-RACE |
Nr1d2 3’-RACE inner | F: AGTGCCGCTTCAAGAAATGC | 3’-RACE |
Nr1d2 3’-RACE inner | F: GGAGATGAGCCTCTTCACTGC | 3’-RACE |
Arntl1 | F: GGCTATCCCTACTCCAACCAG | RT-PCR |
Arntl2 | R: TTGCTGGGGCTGCTGGAA | RT-PCR |
F: AGGGACCCAAATCGCAAATG | ||
Clock | R: TGTGGGGAAACAAGGGGAC | RT-PCR |
F: TGCTGGAGGCTCTGGATGG | ||
Npas2 | R: GGTTCTGGTCCACTAAGTCCGTC | RT-PCR |
F: CAGATAGCGAGTTCAGCCAAGA | ||
Cry1 | R: TGGAGAATGAAGGAGCGATGA | RT-PCR |
F: GAATGCCAACTCACTGCTCG | ||
Cry2 | R: CGAAGCAGGGGTTGTTGG | RT-PCR |
F: GAGAAAAGCGTGGGTGGC | ||
Cry3 | R: CTTGCGGTAGAGGTCTGTGAG | RT-PCR |
F: ATCTTGAAGGACTACCCGAACC | ||
Cry-dash | R: GCTGCCCTCTGCGTGGTTA | RT-PCR |
F: GCCCTGGACCCTCAGCACT | ||
Per1 | R: CCTCTATCCCGATGTTGTTTGG | RT-PCR |
F: CAACAAACTCATCCTCCTGGC | ||
Per2 | R: CGGTGGGTAAACAGGGTAGATT | RT-PCR |
F: TGGTAACGAGTCGCAAGGC | ||
Per3 | R: TCACCAGACTGAAGGCGTTAGA | RT-PCR |
F: CAAAGCCGAGTGAAGGACAG | ||
Nr1d1 | R: GGGTTATCGCTCTGGTTGG | RT-PCR |
F: GCCGTGGTGCTGGTGTCTG | ||
Nr1d2 | R: TTGTTGAGCGTTCGCAGGTC | RT-PCR |
F:TCTCCCCATGTGGACCCTC | ||
Rorα | R: GGTGCGGTCCTTCACATCG | RT-PCR |
F: GGTGGGTTCTACCTGGACTTCC | ||
Tim | R: TGAAGGAGCAGTACGGGAAGAA | RT-PCR |
F: GAAGGCTACAGCAAAGACGGA | ||
Foxk2 | R:CTGGCACTTCAGAATGACGGT | RT-PCR |
F: CCTGAGGTGTCTCGGCAAAA | ||
Mbnl1 | R: TGAGCGATGTTGTCTGGAATG | RT-PCR |
F: AGGTGGACAACGGACGGG | ||
Mrf4 | R: CTTTAGGTGGGGAGGAGGGT | RT-PCR |
F: CCGACCTCTGCTGACCATTC | ||
Mstn | R: GACGCAGAAGACTCACTGGTTT | RT-PCR |
F:GCACATACGCATCCGCTCCCT | ||
Murf1 | R:GTCACGGCCAAGTCATTTCCA | RT-PCR |
F: TCCAGGAACCCCTACCACTACT | ||
Myf5 | R: CACTTCGGCTCTTTGGTGTCTT | RT-PCR |
F:AGGTCAACCACGCTTTCGAG | ||
myoD | R:GTTTTCCACCTGCTCCCGTA | RT-PCR |
F:CAACGACGCCTTTGAGACCCTG | ||
myoG | R:GTCCGAATCCCCGCTGTAGTGT | RT-PCR |
F: GGTGTTGGAGTCGGGGTGA | ||
Pdk4 | R: TGGTAACCGTCTTCCTTTTGC | RT-PCR |
F: CTCTGGTGAACATCCGTAATCG | ||
Pcna | R:ATGGGCTGGGTTCACGCT | RT-PCR |
F: GGACGAGGCGGTCACTATTG | ||
Ucp3 | R: CTGAGGGTGACGGTCTTGGA | RT-PCR |
F: GTATCGGGGAGCGTTTGG | ||
Rpl-13 | R: AGTCCTGCCACCAGTCCGT | internal reference |
F:CACAAGAAGGAGAAGGCTCGGGT | ||
β-actin | R: TTTGGCTCTCTTGGCACGGAT | internal reference |
F:TGCGTGACATCAAGGAGAAGC | ||
| Â | R:GAGGAAGGAAGGCTGGAAGAG | Â |
hprt1 | F:CATACCAAAGCATTACGCAGAAG | internal reference |
R:CACCTCGAATCCTACAAAGTCCG | Â | |
rps29 | F:TCACCCCAGAAAATTCGGACAGG | internal reference |
R:GTATTTACGGATCAGACCGTGTC | Â | |
GAPDH | F:ATCAAGGAAGCGGTGAAGAAGG | internal reference |
R:CGAAGATGGAGGAGTGGGTGTC | Â | |
18Â S rRNA | F:GGAATGAGCGTATCCTAAACCC | internal reference |
R:CTCCCGAGATCCAACTACAAGC | Â |