Fig. 10
The expression of ZbHAA1, but not of ScHAA1 gene, in S. cerevisiae cup2∆ mutant leads to the activation of transcription from S. cerevisiae Cup2 target gene CUP1. a Growth curves of S. cerevisiae BY4741 parental strain transformed with the cloning vector (●,○) and of its derived deletion mutant cup2∆ expressing the recombinant plasmids pG_ZbHAA1 (♦,◇), pG_ScHAA1 (▲,△) or the cloning vector (■, □) cultivated in MM4-U (filled symbols). CuSO4 was added after 1 h of growth in MM4-U to a final concentration of 0.1 mM (empty symbols). For mRNA levels quantification, yeast cells were harvested before CuSO4 supplementation (Time point 0), or 1 or 2 h after the addition of CuSO4 (Time points 1 and 2, respectively). b Comparison of the transcript levels from S. cerevisiae gene CUP1 in time points 0, 1 and 2. ACT1 mRNA level was used as an internal control. The mRNA level from CUP1 gene in S. cerevisiae BY4741 transformed with the cloning vector (black bar) in the absence of CuSO4 (Time point 0) was set as 1 and the transcript levels from CUP1 gene were calculated relative to this control. CUP1 transcript levels in cup2∆ transformed with the cloning vector, pG_ScHAA1 or pG_ZbHAA1-IST are represented by light grey, dark grey or white bars, respectively. mRNA values shown are the mean of, at least, three independent experiments. Results were analysed by two-way ANOVA. (*) p-value below 0.05, when comparing the relative mRNA levels in each strain at a given time point