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Table 2 Duration of the lag phase of growth (h) and maximum specific growth rates (μmax (h−1))

From: The Zygosaccharomyces bailii transcription factor Haa1 is required for acetic acid and copper stress responses suggesting subfunctionalization of the ancestral bifunctional protein Haa1/Cup2

 

0 mM Acetic acid

60 mM Acetic acid

75 mM Acetic acid

Strain

Lag phase (h)

μmax (h−1)

Lag phase (h)

μmax (h−1)

Lag phase (h)

μmax (h−1)

BY4741 + pGREG506_noHIS3

-

0.22 ± 0.016

12.7 ± 3.4

0.22 ± 0.007

35.6 ± 5.4

0.15 ± 0.010

BY4741 + pG_ScHAA1

-

0.22 ± 0.013

8.3 ± 2.2

0.16 ± 0.004

15.4 ± 1.8

0.12 ± 0.004

BY4741 + pG_ZbHAA1-CL

-

0.21 ± 0.023

7.2 ± 2.1

0.20 ± 0.010

11.9 ± 1.0

0.13 ± 0.002

BY4741 + pG_ZbHAA1-IST

-

0.23 ± 0.016

6.3 ± 2.3

0.21 ± 0.018

10.8 ± 0.4

0.13 ± 0.001

haa1∆ + pGREG506_noHIS3

-

0.23 ± 0021

37.1 ± 5.0

0.16 ± 0014

NG

NG

haa1∆ + pG_ScHAA1

-

0.23 ± 0014

7.9 ± 1.2

0.18 ± 0013

15.5 ± 1.9

0.13 ± 0002

haa1∆ + pG_ZbHAA1-CL

-

0.23 ± 0013

8.1 ± 0.9

0.21 ± 0010

14.3 ± 0.0

0.15 ± 0001

haa1∆ + pG_ZbHAA1-IST

-

0.24 ± 0015

9.1 ± 1.2

0.19 ± 0017

12.9 ± 0.7

0.14 ± 0005

  1. Duration of the lag phase of growth (h) induced by acetic acid stress and maximum specific growth rates (μmax (h-1)) of S. cerevisiae BY4741 and derived deletion mutant haa1∆ expressing ZbHAA1 from Z. bailii strains IST302 (pG_ZbHAA1-IST) and CLIB 213T (pG_ZbHAA1-CL), S. cerevisiae HAA1 (pG_ScHAA1) or the cloning vector (pGREG506_noHIS3). Mean and standard deviation values of at least three independent experiments are indicated. (−) No lag phase detected; (NG) No growth detected during the course of the experiment (65 h)
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BMC Genomics

ISSN: 1471-2164

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