Fig. 6

Expression analysis of candidate phenylpropanoid genes in four soybean cultivars. Total RNA was extracted from the roots of soybean cultivars Conrad, AC Colombe, AC Glengarry and Pagoda, and was used in qPCR analysis. Expression of the following genes was analyzed: GmF6H3, GmI2′H, GmF3′H, GmF3′5′H and GmDFR, using specific primers. Relative expression corresponds to mean gene expression in three biological replicates, with technical triplicates. Error bars indicate SEM. Values were normalized against the reference genes GmCONS4 and GmCONS6. Asterisks (*) indicate significant differences between the samples as determined by Student’s t -test