Fig. 3

Expression of HBD1 and HBD2 mRNA in adults. a Primers specific for HBD1 and HBD2 5′-untranslated regions were used with a primer to shared sequence in exon 3 to conduct PCR amplification of adult liver cDNA (along with controls of genomic DNA and no DNA template). b Standard dideoxynucleotide sequencing was used to test for the single non-primer position that differs between the two amplicons. Sequencing with the exon 3 primer showed the HBD1 and HBD2 cDNA amplicons are pure and unique (i.e., the respective diagnostic base is present in each), showing the two genes are expressed