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Expansion and stress responses of the AP2/EREBP superfamily in cotton

BMC Genomics volume 18, Article number: 118 (2017) Cite this article

Abstract

Background

The allotetraploid cotton originated from one hybridization event between an extant progenitor of Gosssypium herbaceum (A1) or G. arboreum (A2) and another progenitor, G. raimondii Ulbrich (D5) 1–1.5 million years ago (Mya). The APETALA2/ethylene-responsive element binding protein (AP2/EREBP) transcription factors constitute one of the largest and most conserved gene families in plants. They are characterized by their AP2 domain, which comprises 60–70 amino acids, and are classified into four main subfamilies: the APETALA2 (AP2), Related to ABI3/VP1 (RAV), Dehydration-Responsive Element Binding protein (DREB) and Ethylene-Responsive Factor (ERF) subfamilies. The AP2/EREBP genes play crucial roles in plant growth, development and biotic and abiotic stress responses. Hence, understanding the molecular characteristics of cotton stress tolerance and gene family expansion would undoubtedly facilitate cotton resistance breeding and evolution research.

Results

A total of 269 AP2/EREBP genes were identified in the G. raimondii (D5) cotton genome. The protein domain architecture and intron/exon structure are simple and relatively conserved within each subfamily. They are distributed throughout all chromosomes but are clustered on various chromosomes due to genomic tandem duplication. We identified 73 tandem duplicated genes and 221 segmental duplicated gene pairs which contributed to the expansion of AP2/EREBP superfamily. Of them, tandem duplication was the most important force of the expansion of the B3 group. Transcriptome analysis showed that 504 AP2/EREBP genes were expressed in at least one tested G. hirsutum TM-1 tissues. In G. hirsutum, 151 non-repeated genes of the DREB and ERF subfamily genes were responsive to different stresses: 132 genes were induced by cold, 63 genes by drought and 94 genes by heat. qRT-PCR confirmed that 13 GhDREB and 15 GhERF genes were induced by cold and/or drought. No transcripts detected for 53 of the 111 tandem duplicated genes in TM-1. In addition, some homoeologous genes showed biased expression toward either A-or D-subgenome.

Conclusions

The AP2/EREBP genes were obviously expanded in Gossypium. The GhDREB and GhERF genes play crucial roles in cotton stress responses. Our genome-wide analysis of AP2/EREBP genes in cotton provides valuable information for characterizing the molecular functions of AP2/EREBP genes and reveals insights into their evolution in polyploid plants.

Background

The APETALA2/ethylene-responsive element binding protein (AP2/EREBP) superfamily is one of the largest and specific transcription factor (TF) families in plants. Members are defined by their AP2/ERF domain, which comprises about 60 to 70 amino acids [1–3]. They play essential roles in plant growth, development and responses to various environmental stresses, including cold, heat, drought, high salinity and pathogen infection, by directly responding to stresses or regulating the expression of downstream target genes [4–7]. The AP2/EREBPs have also been implicated in different hormones-related signal transduction pathways including ethylene, abscisic acid (ABA), cytokinin and jasmonate (JA) [2, 8–10].

Since the release of the whole-genome sequences of many plant species, the AP2/ERFEBP superfamily has been successfully identified and investigated in a variety of plant species, such as Arabidopsis (147) [11], poplar (200) [12], soybean (98) [13], tomato (85) [14], rice (163) [15], potato (155) [16], Medicago truncatula (123) [17] and Brachypodium Distachyon (149) [18]. All these AP2/EREBP genes are characterized by one or two AP2 DNA binding domains, which consist of a three-stranded anti-parallel β-sheet and an α-helix [19]. In the model plant Arabidopsis, the 147 AP2/EREBP genes are divided into four subfamilies: the APETALA2 (AP2), Dehydration-Responsive Element Binding protein (DREB), Ethylene-Responsive Factor (ERF) and Related to ABI3/VP1 (RAV) subfamilies [20]. The AP2 subfamily members, which contain two AP2 domains, have important functions in the regulation of plant growth and development [21–26]. The RAV subfamily transcription factors, which possess a single AP2 domain and an additional B3 domain [27], as well as a DNA-binding domain commonly found in other TFs [28], play significant roles in the regulating expression of target genes in response to ethylene, brassinosteroids and environmental stresses [29–32]. The ERF and DREB subfamily members, which contain a single conserved AP2 domain and comprise the largest groups in the AP2/EREBP superfamily, play critical roles in stress responses and in a variety of other plant processes. Additionally, a fifth group comprises all other AP2/EREBPs not assigned to the other four groups: there is only one gene (At4g13040) in this group in Arabidopsis [11].

Sequences contained only one AP2 domain had the greatest number of members in the AP2/EREBP superfamily, which are further divided into two major subfamilies, the ERF and DREB subfamilies [33]. The ERF subfamily members directly bind to GCC-boxes (AGCCGCC) and regulate the expression of pathogenesis-related (PR) genes. In addition, the ERFs are involved in hormone signaling pathways, such as the ethylene, JA, and salicylic acid (SA) pathways, which are important for plant development and stress responses [34–37]. The DREB subfamily members bind to the dehydration-responsive element/C-repeat, A/GCCGAC (DRE/CRT) elements, which are present in stress-responsive genes such as RD29 and COR15 genes [38, 39]. They regulate stress-responsive genes in response to various abiotic stresses, including cold, heat, drought and salinity [28, 40, 41]. In Arabidopsis the ERFs and DREBs were further divided into 12 groups, A1-A6 (the DERB subfamily) and B1-B6 (the ERF subfamily) by Sakuma et al. [33]. Recently, a new group B7 was identified in the ERF subfamily in rice and Brachypodium Distachyon [15, 18], indicating that the ERF genes may have further functions in plants.

Cotton is one of the most important economic crops worldwide and provides the world’s leading natural textile fiber and considerable amounts of edible oil. Studies have shown that allotetraploid Gossypium species were formed by a polyploidization event that occurred 1 ~ 1.5 million years ago (Mya), involving an A-genome species G. arboreum and a paternal D-genome species G. raimondii [42]. As a young allotetraploid plant, G. hirsutum is an excellent model plant for research into the functional divergence and evolution of duplicated genes. Since the release of the whole-genome sequences of the diploid cotton G. raimondii [43] and the allotetraploid cotton G. hirsutum (TM-1) [42], genome-wide analysis of AP2/EREBP genes is possible, and will help to elucidate their regulatory functions in plant growth, development and, in particular, stress responses. Although many other gene families and some members of the AP2/EREBP genes have been studied in cottons, the AP2/EREBP superfamily remains largely unexplored in cotton. Genomic analysis is an effective means of transferring knowledge from one taxon to another [44], and can facilitate our understanding of the expansion and functions of the AP2/EREBP superfamily genes in the evolution of cotton. In this study, we performed a comprehensive analysis of the AP2/EREBP superfamily in cotton, including phylogenetic tree, chromosomal localization, gene structure, gene expansion, and synteny analyses, as well as investigations into the expression profiles of these genes in various tissues and their expression patterns under different stresses. The results will help future investigations aimed at the functional characterization of stress tolerant AP2/EREBP genes, and can be utilized in the genetic improvement of cottons and studies into their genetic evolution.

Results

Genome-wide identification of the AP2/EREBP superfamily in cotton

The allotetraploid cotton species appeared about 1–1.5 Mya through the hybridization of a maternal A-genome species and a paternal D-genome species [42]. The whole genome sequence scaffolds of two sequenced cotton species (G. raimondii [43] and G. hirsutum acc. TM-1 [42]) were used for the genome-wide exploration of the AP2/EREBP gene family in Gossypium. Using the Hidden Markov Model (HMM) (HMMER v3.0) method with data from a query on the AP2/EREBPs family (PF00847), we searched the protein databases, and obtained a total of 269 AP2/EREBPs in G. raimondii after confirming the presence of the ‘AP2’ domain (Additional file 1: Table S1). As a young polyploidy species, genes from parental genomes were mostly retained in the G. hirsutum subgenomes [42]. In this study, we identified 504 AP2/EREBPs, 252 in the A subgenome and 252 in the D subgenome, in G. hirsutum acc. TM-1 (Additional file 2: Table S2). The homologous between G. raimondii and G. hirsutum showed high similarity and clustered together on the phylogenetic tree with one gene from G. raimondii and two from G. hirsutum (Additional file 3: Figure S1). The number of AP2/EREBPs in allotetraploid cotton has nearly doubled compared to that in diploid cotton, although a handful of homologs have been lost. We named the AP2/EREBP genes in G. hirsutum corresponding to their relationships to that of in G. raimondii, and marked A or D after each gene name to represent these genes belonging to A-subgenome or D-subgenome.

Classification and phylogeny of the AP2/EREBP genes in Gossypium

Based on sequence similarities, the composition of domains and the number AP2 domain, the 269 AP2/EREBP genes in G. raimondii were divided into four groups. Specifically, four genes were grouped as outsiders with relatively complex sequences; 32 genes containing two complete AP2 domains were assigned to the AP2 subfamily; 11 genes had a single AP2 domain and a single B3 domain and were classified into the RAV subfamily; and the remaining 222 genes carried a single AP2/ERF domain and were assigned to the DREB/ERF subfamilies. The DREB/ERF subfamily members were further divided into DREB (80) and ERF (142) subfamilies based on sequence similarities (Additional file 4: Figure S2). Excluding the four outsider genes, the 265 AP2/EREBP genes were named GrAP2-1-32, GrDREB1-80, GrERF1-142 and GrRAV1-11, respective to their subfamilies (Additional file 1: Table S1).

In order to appreciate the phylogenetic relationships of the AP2/EREBP genes in G. raimondii, we employed MEGA v5.2 software to construct an unrooted phylogenetic tree of AP2/EREBPs from G. raimondii and A. thaliana (Additional file 5: Figure S3). The phylogenetic tree clearly showed that the remaining 265 genes were clustered into four subfamilies, the RAV, AP2, DREB and ERF clades, comprising 11, 32, 80 and 142 proteins, respectively (Fig. 1). This was consistent with the above classification that was based on domain compositions and the number of AP2 domains.

Fig. 1
figure 1

Phylogeny trees of the G. raimondii AP2/EREBP superfamily genes. a Phylogeny of the G. raimondii AP2 (APETALA2) and RAV (Related to ABI3/VP1) subfamily proteins based on their conserved domain sequences. b Phylogeny of the G. raimondii DREB (dehydration responsive element binding gene) and ERF (ethylene responsive factor) subfamily proteins based on their conserved domain sequences. Different subgroups of DREB and ERF subfamily proteins are highlighted in different colors

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The classifications of the AP2/EREBP genes were used to infer the phylogenetic history of the DREB/ERF genes and AP2 and RAV genes, respectively (Fig. 1). As shown in the phylogenic tree, the 32 AP2 genes and the 11 RAV genes were divided into two subfamilies based on their sequence similarities and the number of AP2/ERF domains in the encoded proteins (Fig. 1a). The remaining 222 genes were classified into two subfamilies: the DREB (80 genes) and ERF (142 genes) subfamilies (Fig. 1b). There were two conserved amino acids distinguished between the DREB and ERF genes (Additional file 4: Figure S2). The 19th glutamic acid (E19), and, in particular, the 14th valine (V14) were conserved in the DREB proteins, whereas alanine (A14) and aspartic acid (D19) were conserved in the ERF proteins (Additional file 4: Figure S2). The DREBs specifically bound to six nucleotides (A/GCCGAC) of DRE, while the ERFs were found to be involved in ethylene-responses and specifically bound to the GCC box (AGCCGCC) [33]; indicating the functional divergence of the DREB and ERF subfamilies in plants.

Moreover, the DREB subfamily was further divided into six groups (A1 to A6) as in Arabidopsis [11], of which the A4 group was the largest and the A3 group was the smallest with only one member (Gorai.011G291900) (Fig. 1b). Likewise, the ERF subfamily was classified into seven groups as in rice [15] and Brachypodium Distachyon [18]. The group B3 contained 63 (44% of the 142 ERFs) members and was the largest (Fig. 1b). Interestingly, the number of ERF subfamily members was almost double that of the DREB subfamily members. This indicated that each AP2/EREBP subfamily evolved to have species-specific characteristics and the ERF subfamily may have experienced fast gene expansion.

The ERF subfamily members included more than half of the AP2/DREBP superfamily members, including five genes (GrERF23, GrERF83, GrERF82, GrERF136 and GrERF55) that had not been reported before and could not be allocated into any existing group (B1 to B6), so were classified into a new group, B7, as in rice and Brachypodium Distachyon [15, 18]. These genes may have new functions related to human selection pressure during cotton domestication.

Chromosomal locations of GrAP2/EREBP genes

To examine the genomic distribution of AP2/EREBP genes on cotton chromosomes, we identified their positions. The 265 cotton AP2/EREBP genes were scattered unevenly over the 13 chromosomes, and were distributed individually or in clusters (Fig. 2). Chromosome D12 had the largest number (38) of AP2/EREBP genes, while chromosome D4 had the smallest, with only six members (GrAP2-29, GrAP2-30, GrAP2-31, GrDREB78, GrERF134 and GrERF135). Interestingly, many genes were distributed in clusters, especially at the telomeric ends of chromosomes 5, 7, 8, 9, 11 and 12 (Fig. 2). Such uneven distribution of these genes provided a clue to their evolution.

Fig. 2
figure 2

Chromosomal distributions of the G. raimondii AP2/EREBP genes. The chromosome numbers were consistent with those in the interspecific genetic map (D1 to D13) of allotetraploid cultivated cotton species and the scaffolds (Chr.1 to Chr.13) of the G. raimondii genomic data [43]. The nomenclature of the AP2/EREBP genes was based on the order of the chromosomes in G. raimondii. Grey lines indicate tandem duplicated genes. Genes are color coded according to their subfamily

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Some genes distributed in clusters were tandem duplicated genes. We analyzed possible tandem duplication events in the G. raimondii AP2/EREBP family and found that 73 AP2/EREBP genes belonging to 28 gene clusters were involved in tandem duplication (Fig. 2). The number of GrAP2/EREBP duplicated genes arranged in tandem repeats varied from two to eight, and the largest gene cluster consisted of eight tandem duplicated genes (GrERF12-19) on chromosome D7. There were 57 tandem duplicated genes belonging to group B3 of the ERF subfamily (Fig. 2 and Additional file 6: Table S3). Additionally, there were six genes (three pairs of RAVs) originating from tandem duplication events. This is a large proportion for a relatively small subfamily.

The exon/intron organizations of GrAP2/EREBP genes are relatively simple

To obtain a deep insight into the gene structures of the cotton AP2/EREBP genes, their intron and exon structures were analyzed. We found that the AP2/EREBPs had a relatively simple gene structure, with the exception of the AP2 subfamily members (Additional file 7: Figure S4). All members of the AP2 subfamily had five to nine introns, while the majority of members of the RAV, DREB and ERF subfamilies had no introns and relatively simple structures. All members of the DREB and RAV subfamilies had no introns, with the exception of GrDREB7, GrDREB13, GrDREB24, GrDREB28, GrDREB54, GrDREB57, GrDREB67, GrDREB74 and GrRAV5, which had one intron, and GrDREB15 and GrRAVA4, which had two introns. In the ERF subfamily, all members of the groups B2 and B4 had one intron and most members of the other B groups had no introns or one intron, with the exception of GrERF58 and GrERF105, which had two introns. In addition, the 63 members of the largest group, B3, had no introns, with the exception of GrERF13, GrERF16, GrERF39, GrERF69, GrERF78, GrERF99 and GrERF134, which had only one intron each (Additional file 7: Figure S4). Genes originating from tandem duplications were clustered together in the phylogenetic tree and shared similar structural organizations (Additional file 7: Figure S4).

AP2/EREBP superfamily expansion in Gossypium

Tandem and segmental duplications are known to be major forces for expansion of gene families in plants. Studies showed it is important of gene family expansion of plant tandem duplicates in the adaptive response to environmental stimuli [45, 46]. Large-scale duplication events are predicted to have occurred during Gossypium evolution [43]. We analyzed the possible tandem and segmental duplication events in the G. raimondii AP2/EREBP family and found 73 genes in 28 tandem gene clusters distributed unevenly on ten cotton chromosomes (Fig. 2), as well as 221 segmental duplicated gene pairs in 185 blocks, which were found throughout the genome (Fig. 3).

Fig. 3
figure 3

Synteny relationships between AP2/EREBP genes in G. raimondii. Blue lines show duplications with Ks >1.5 and green lines show duplications with Ks <1.5

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There were 57 tandem duplicated genes belonging to group B3 of the ERF subfamily and they contributed 82.6% of all the duplicated genes (Fig. 2 and Additional file 6: Table S3). The largest gene cluster consisted of eight tandem duplicated genes on chromosome D7 (Fig. 2). Tandem duplications made a great contribution to the expansion of the group B3 of the ERF subfamily.

To further investigate the AP2/EREBP gene family expansion pattern in G. raimondii, we download the syntenic data of G. raimondii from the Plant Genome Duplication Database (PGDD) and analyzed the gene duplication pattern of AP2/EREBPs from different phylogenetic subfamilies. AP2/EREBPs with syntenic relationships were detected in all subfamilies, indicating extensive segmental duplications throughout the genome (Fig. 3). In total, there were 221 pairs of genes with syntenic relationships in 185 syntenic blocks. Of them, there were 83 pairs of DREB, 112 pairs of ERF, 22 pairs of AP2 and four pairs of RAV subfamily genes (Fig. 3). In summary, Gossypium lineage segmental duplication events contributed to the expansion of all four subfamilies. However, most of the tandem duplicated genes (82.6%) belonged to group B3 of the ERF subfamily and contributed to its expansion.

Furthermore, we analyzed the substitution per synonymous site (Ks) values of 221 segmental gene pairs in G. raimondii and observed two peaks at Ks values of 0.5–0.8 and 1.5–2.3 (Fig. 4 and Additional file 8: Table S4). The first peak appeared at approximately 60 Mya, corresponding to the whole-genome duplication (WGD) event that was previously proposed in the Gossypium lineage [43, 47]. The second peak appeared at about 130.8 Mya, corresponding to the paleohexaploidization event shared by the eudicots [43, 48–50]. The Ks values for each pair of genes within a syntenic block were used to interpret duplication events (Additional file 8: Table S4). The 64 paralog gene pairs with Ks values ranged from 1.50 to 2.45, may be derived from the ancient hexaploidization event about 130.8 Mya [46, 50]. The remaining 157 paralog gene pairs, which had Ks values ranged from 0.45 to 1.08, were likely to have originated from the Gossypium lineage duplication events [43, 47]. Interestingly, we found that no RAV subfamily genes were retained after the ancient hexaploidization event, while all four pairs of RAVs (GrRAV1 & GrRAV11, GrRAV2 & GrRAV8, GrRAV2 & GrRAV3 and GrRAV3 & GrRAV8) with syntenic relationships originated from the recent Gossypium lineage duplication events.

Fig. 4
figure 4

Frequency distribution of Ks values of segmental duplicated gene pairs in G. raimondii. The x axis denotes the Ks value. The y axis denotes the relative frequency

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Synteny comparison of AP2/EREBP subfamily to cacao and grape

A genomic analysis of the synteny of the AP2/EREBP gene family across Theobroma cacao and Vitis vinifera could provide insights to its expansion and evolution. We comparatively analyzed the synteny across G. raimondii, T. cacao and V. vinifera (Additional file 9: Figure S5). There are 312 syntenic gene pairs that were syntenic between G. raimondii and T. cacao, while there are 127 pairs between G. raimondii and V. vinifera (Additional file 9: Figure S5). The greater number of segmental duplicated paralogous gene pairs between G. raimondii and T. cacao indicates the closer relationship between G. raimondii and T. cacao than that between G. raimondii and V. vinifera. These results were consistent with the evolutionary relationships already identified between these species [43].

In this study, we identified a total of 269 AP2/EREBP genes in the diploid cotton G. raimondii, 102 in grape and 123 in cacao. These 269 AP2/EREBPs represented 0.72% of the whole genome of G. raimondii, a ratio much higher than the 0.39% in grape [24], 0.55% in Arabidopsis [11] and 0.42% in cacao, suggesting that the expansion of the AP2/EREBPs in cotton was greater than that of in other dicot plants. This expansion may have benefited from the Gossypium lineage WGD events [43, 47]. Furthermore, we compared the proportion of genes in each of the subfamilies in G. raimondii, cacao and grape and found that the number of genes in the DREB, ERF and RAV subfamilies increasing during the divergence of the species. However, the number of AP2 subfamily genes decreased gradually, while the number of outsider genes remained unchanged (Additional file 10: Figure S6). These results indicate the expansion of the AP2/EREBP superfamily was mainly due to the expansion of DREB and ERF subfamilies by segmental or tandem duplications, and this expansion may have enhanced the wide adaptability of cotton.

Spatio-temporal expression profiles of GhAP2/EREBP genes in G. hirsutum

AP2/EREBP genes play essential roles in plant growth and development including root initiation in rice [51], ovule and sepal development in Arabidopsis [22, 52], fruit development and ripening process in tomato [23] and grapevine growth [24]. We used our high-throughput sequencing data from G. hirsutum acc. TM-1 [42] to investigate the expression profiles of AP2/EREBP family genes in various G. hirsutum tissues, including roots, stems, leaves, −3 days post anthesis (dpa), 0 dpa and 3 dpa ovules and 5 dpa, 10 dpa, 20 dpa and 25 dpa fibers. There were 504 AP2/EREBP genes expressed at least in one tested tissue. To further elucidate the transcription patterns of GhAP2/EREBP genes, their expression patterns were clustered across each subfamily and different groups. In general, different subfamilies showed different expression patterns (Fig. 5), suggesting the functional divergence of different subfamilies and groups of GhAP2/EREBP members.

Fig. 5
figure 5

Expression profiles of GhAP2/EREBP genes in various tissues. Ten tissues comprising roots, stems, leaves, −3dpa ovules, 0dpa ovules, 3dpa ovules, 5dpa fibers, 10dpa fibers, 20dpa fibers and 25dpa fibers were investigated. Expression profiles (in log2 based values) of the GhDREB (a), GhERF (b), GhAP2 (c) and GhRAV (d) genes in three tissues. Scale bars represent log2 of the RPKM values

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The DREB genes, showed various expression patterns and functional divergence in vegetative and reproductive organs (Fig. 5a). Five genes in group A1 were highly expressed in roots, −3 dpa ovules and 0 dpa ovules, as well as in 25 dpa fibers. Two genes (GhDREB52A/D) were expressed in all of the ten tissues investigated, except 5 pda and 10 dpa fibers. Three genes were only expressed in one or two tissues. In addition, two pairs of homoeologous genes (GhDREB52A/D and GhDREB55A/D) showed obvious subgenome expression bias in different tissues (Fig. 5a), revealing the functional divergence of homoeologous gene pairs in allotetraploid cotton. Only four genes in the groups A2 and A3 were highly expressed in only one tissue. And in the groups A5 and A6, more genes were constitutively expression in all tissues. The A4 group contained the greatest number of members but about half of them showed very little expression (Fig. 5a).

The ERF members represented 52.8% (142 genes) of the whole AP2/EREBP family and had the greatest number of expression profiles in various tissues (Fig. 5b). Almost all genes in group B2, and half of the members of group B1, showed constitutive expression, but the group B4 members were only detected in roots or leaves and had lower expression levels. In contrast, members of the groups B3, B5, B6 and B7 showed relatively diverse expression patterns (Fig. 5b). For example, members of group B3, the largest group in the AP2/EREBP family, showed very little expression in any tissue or showed biased expression of one of the homoeologous gene. As a newly classified group [15], members of the group B7 showed very high or very low expression levels, and there was a great divergence in function between those in A- and D-subgenomes (Fig. 5b). The ERF subfamily contributed greatly to the expansion of the AP2/EREBP family, and the genes in this group showed various expression patterns indicating functional divergence during the long period of evolution.

The AP2 genes have been reported to play essential roles in plant growth and development [14, 22–24, 51, 52]. Here, we found 15 genes that were constitutively expressed, 17 genes expressed in the early ovule stage, and ten genes that were mainly expressed in 25 dpa fibers (Fig. 5c). At present, little is known about the role of RAV genes in plant growth and development. Reports have showed the RAV genes may mediate plant responses to auxin, which is involved in plant development [27, 53, 54]. Here, we found only five genes that were expressed mainly in vegetative organs and only one that was specific to 10 dpa fibers. Of them, the GhRAV1D gene was most highly expressed in 10 dpa fibers and may therefore play a role in fiber elongation (Fig. 5d).

Many GhDREB/ERF genes were induced expression under abiotic stresses

To investigate the potential functions of the GhAP2/EREBP genes under various environmental stresses, we used RNA-seq data [42] to detect their expression levels under cold, heat and drought conditions. A total of 151 GhDREB and GhERF genes, including 60 GhDREBs and 91 GhERFs, which were induced by stress treatments were subjected to expression analysis under these stresses. Of these, the expression of 132 genes was induced by cold, 94 genes by heat and 63 genes by drought (Additional file 11: Figure S7). Therefore, the greatest number of these genes was induced by cold and the lowest by drought.

The GhDREB subfamily genes could be classified into two subgroups based on their expression profiles in cold conditions. In the first group, the peak of GhDREB genes expression occurred as late as 12 or 24 h after stress treatment, while the other group of GhDREB genes had an expression peak 24 h after the treatment. In addition, 11 of these genes showed constitutive expression (Fig. 6a). In some of the homoeologous gene pairs, only one gene was induced, while the other was not detected, indicating that it may lost its function after the formation of allotetraploid cotton. There were three main expression patterns of the GhERF genes. In the first group, they responded rapidly in the early stage of induction and reached a peak at 6 h (for example GhERF125A and GhERF82D) (Fig. 6a); in the second group, they were induced in the early stage, then their expression declined, before increasing to reach a second peak; and in the third group, they were induced relatively slowly, and reached an expression peak at 24 h or more after stress treatment (Fig. 6a).

Fig. 6
figure 6

Expression patterns of GhDREB/ERF genes under various stresses. Expression profiles (in log2 based fold change) of GhDREB and GhERF genes under different abiotic stress conditions: including a cold, b heat and c drought. Scale bars represent log2 of the RPKM values

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The number of genes induced by heat treatment were lower than that in cold and drought treatments, and they showed different expression levels and different durations of expression. With the Exception of a small number of GhDREB genes, which were expressed continuously, most of the other genes had two expression peaks at 1 h and 24 h after treatment (Fig. 6b). The genes induced by heat were divided into two groups based on their expression profiles; those that reached a peak at 6 h and continued being highly expressed until at least 24 h after treatment, and those that were mainly expressed at 3 h and 24 h after treatment (Fig. 6c). GhDERB genes are known to mediate changes in gene expression in drought and low temperature conditions [55]. Here, we found that most of the GhDREB genes were rapidly induced during early stages of drought treatment. The expression of some of GhDREB genes then decreased, while some others increased over time (Fig. 6c). These results suggest that different GhDREB genes play different roles in drought responses. Some of the GhERF genes were also induced by drought at early or late stages, this showed various expression patterns of GhERF genes (Fig. 6c). Multiple stresses induced expressions were found between GhDREB and GhERF genes under different stress treatments, indicating that the GhDREB and GhERF genes may be involved in a crosstalk between signal transduction pathways in response to different abiotic stresses, or that some functions of different genes are complementary.

We also examined the expression of 28 selected genes (13 GhDREBs and 15 GhERFs) using quantitative reverse transcription PCR (qRT-PCR) under cold and drought stress treatments. 17 genes were induced by both cold and drought stresses. All of the 28 selected genes were significantly induced by stress at one or more time point/s (Figs. 7 and 8), and this was consistent with the RNA-Seq data. Overall, the gene response was slower in cold conditions than in drought conditions. Expression levels gradually increased over time in low temperature conditions and reached a peak at 12 h or 24 h (Fig. 7). In contrast, genes responded immediately to drought, with expression levels peaking 1 or 3 h after treated (Fig. 8). These results indicate that the expression patterns of GhDREB/ERF genes under cold damage might improve the environmental adaptability of cotton to high latitude regions with relatively lower temperatures.

Fig. 7
figure 7

Validation the expression of the selected GhDREB/ERF genes in response to cold using qRT-PCR. The mean expression values were calculated from three independent replicates. 0, 1, 3, 6, 12 and 24 h indicate the number of hours after treatment. Mean values and standard errors were calculated from three replicates. The asterisk and double asterisks represent significant differences at the levels of 0.05 and 0.01, respectively. R.e.l indicates Relative expression level

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Fig. 8
figure 8

Validation the expression of the selected GhDREB/ERF genes inresponse to drought using qRT-PCR. The mean expression values were calculated from three independent replicates. 0, 1, 3, 6, 12 and 24 h indicate the number of hours after treatment. Mean values and standard errors were calculated from three replicates. The asterisk and double asterisks represent significant differences at the levels of 0.05 and 0.01, respectively. R.e.l indicates Relative expression level

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Functional divergence of GhAP2/EREBP genes

Gene duplication is a major force of genetic novelty and can lead to evolutionary innovation. Three possible fates of duplicated genes have been suggested as non-functionalization, sub-functionalization and neo-functionalization [56]. Gene expression patterns analysis may provide a lot of information for studying the functional divergence and evolution of homoeologous genes. Using RNA-seq data [42], the expression patterns of homoeologous gene pairs in cotton were compared to gain insights into their functional divergence during evolution.

Most of the homoeologous gene pairs showed no expression bias between homoeologs. These genes may retain their original function based on the gene dosage. But some homoeologous gene pairs with one member lost or silenced showed non-functionalization in allotetraploid cotton. For example, GhDREB2A, GhERF55A and GhRAV3D had function in specific organs, while their homoeologs was silenced (Additional file 12: Figure S8a). Possible explanations for this are many and varied, but include the preservation of appropriate gene dosage and the requirements imposed by interacting and divergent regulatory hierarchies [57, 58].

There were also some gene pairs that had partitioned aggregate ancestral functions (sub-functionalization), for example, it is showed half gene dosage of each member of the GhDREB12, GhDREB77 and GhERF81 homoeologous gene pairs (Additional file 12: Figure S8b). However, the GhAP2-31 and GhDREB26 homoeolog pairs showed expression level dominance in different tissues. The GhAP2-31A and GhDREB26D genes had lower expression levels in vegetative organs, such as roots, stems and leaves than those of GhAP2-31D and GhDREB26A, but had higher expression levels in reproductive organs including ovules and fibers (Additional file 12: Figure S8c). These results indicate non-functionalization and sub-functionalization of homoeologous gene pairs during cotton evolution.

We also noticed that one member of the homoeologous gene pairs were differentially induced by different stress may indicate neo-functionalization of one member. For example, the homoeologous gene pairs of GhDREB1, GhDREB7, GhDREB12, GhDREB33, GhDREB40, GhDREB56, GhERF70, GhERF109 and GhERF113 were both expressed in various tissues but only one member such as GhDREB1A, GhDREB12D, GhDREB33D, GhERF70D and GhERF109A were induced by cold stress, GhDREB56A and GhERF113D were induced by heat stress, GhDREB40D was induced by drought stress and GhDREB7A was induced by drought, heat and cold stresses (Figs. 5 and 6). One member of these homoeologous gene pairs may gain new function (neo-functionalization) in cotton stress tolerance.

Additionally, we analyzed the expression patterns of 111 tandem duplicated genes from 41 tandem clusters and detected no transcripts for almost half of them in select tissues (Additional file 13: Figure S9). No transcripts were detected for either member of some tandem duplicated pairs, for example, GhAP2-11A and GhAP2-12A, GhAP2-11D and GhAP2-12D, GhERF64A and GhERF65A, GhRAV6A and GhRAV7A, GhRAV9A and GhRAV10A, GhRAV6D and GhRAV7D, GhERF62A and GhERF63A, GhERF39D and GhERF40D, GhERF64D and GhERF65D, GhRAV10D and GhRAV9D and GhERF62D and GhERF63D. In contrast, there were some tandem duplicated clusters in which all members were expressed, for example, GhERF41A, GhERF42A, GhERF43A and GhERF44A, GhERF125A and GhERF126A, GhERF69A and GhERF70A, GhERF71A and GhERF72A, GhERF75A and GhERF76A, GhERF98A, GhERF99A and GhERF100A, GhERF4A, GhERF5A and GhERF6A, GhERF41D, GhERF42D, GhERF43D and GhERF44D, GhERF125D and GhERF126D, GhERF69D and GhERF70D, GhERF71D and GhERF72D, GhERF75D and GhERF76D, GhERF98D, GhERF99D and GhERF100D, GhERF4D and GhERF5D and GhERF6D. Transcripts of only some members of the remaining tandem duplicated gene clusters were detected (Additional file 13: Figure S9). This indicates that some tandem duplicated genes in the young allotetraploid cotton are functionally redundant and might have lost or gain functions during the long domestication process.

In short, the expression analysis of duplicated genes in the A- and D-subgenomes (homoeologous genes) and tandem duplicated genes revealed their functional roles for non-functionalization, sub-functionalization and neo-functionalization during the expansion of the AP2/EREBP superfamily in G. hirsutum. In other words, the functions of the superfamily genes were expanded and enhanced by gene duplication and genome doubling.

Discussion

EREBP genes expanded obviously in cotton

WGD or polyploidy, which results in massive silencing and elimination of duplicated genes, has long been recognized as a significant force in plant evolution [59]. In this study, we identified 269 AP2/EREBP genes in G. raimondii, representing 0.72% of the annotated proteins in this species. The higher ratio (0.72%) suggested expansion of the AP2/EREBP family in Gossypium. The ratios of RAV and AP2 subfamily genes were nearly the same as that in Arabidopsis [11] and poplar [12], showing a strong conservation of these subfamilies and suggesting that the RAV and AP2 genes in all of these plants share a common ancestor prior to the separation of raimondii from the other plants. However, the number of DREB and, in particular, the ERF genes in cotton changes significantly, indicating that they have independently expanded after the cotton differentiation from other species. The group B3 contained the greatest number of members (63 genes) of all the gene groups and expanded mainly form tandem duplication. This indicates the B3 group of the ERF subfamily may experience special functional divergence during cotton evolution. Of the 73 tandem duplicated genes in G. raimondii, 57 members belonged to group B3 of the ERF subfamily. The remaining 16 genes comprised three pairs of RAVs (RAV4 & RAV5, RAV6 & RAV7, RAV9 & RAV10,), one pair of AP2s (AP2-11 & AP2-12) and two pairs of ERFs (ERF73 & ERF74) (Fig. 2). Studies showed it is important of gene family expansion of plant tandem duplicates in the adaptive response to environmental stimuli [45, 46]. The expansion of the ERFBP gene family is an important force for functional divergence to stress response, especially of the B3 group of the ERF subfamily. These genes provided clues to the evolution of tandem duplicated genes and stress tolerance improvement of cotton.

Simple gene structures may reveal high conserved functions

The domain architecture and intron/exon structure of the AP2/EREBP genes in G. raimondii were relatively simple and highly conserved within each subfamily (Additional file 4: Figure S2 and Additional file 7: Figure S4). The DREB and ERF subfamily genes were mainly classified based on two conserved amino acids, the 14th valine (V14) and the 19th glutamic acid (E19) of the DREB proteins, which were alanine (A14) and aspartic acid (D19) of the ERF proteins (Additional file 4: Figure S2). The valine (V14) was present in all the DREB members but the glutamic acid (E19) was not, indicating functional divergence of the A groups. For example, GhDREB76 (A19) and GhDREB79 (V19) of group A2 showed different expression patterns in various tissues (Fig. 5a). In addition, in the A5 and A6 groups, there were only six DREB genes that contained E19; the others contained A19 or L19 (Additional file 4: Figure S2). This is consistent with the expression patterns of the two groups with more members than the other groups, which were highly expressed in all selected tissues (Additional file 4: Figure S2a). The results imply high levels of conservation of V14 in the DREB genes, but divergence in the 19th amino acid contributed to functional variation. In general, there were few differences observed between the ERF members, with the exception of group B6, in which one or two conserved amino acids changed (Additional file 4: Figure S2b). Interestingly, the expression patterns of group B6 members also had distinctive characteristics. In detail, most of the genes in group B6 were specifically expressed in one or several organs (Fig. 5b). This indicates that functional divergence between DREB and ERF genes occurred via change in critical amino acids during cotton evolution and provides excellent candidate genes for researching cotton developmental regulation.

Excellent candidates for cotton improvement

In this paper, we identified some important candidate genes which were highly or specific expressed in some tissues, such as GhDREB77 in fiber maturation, GhDREB2 in −3 dpa ovules involved in fiber initiation (Additional file 12: Figure S8), GhERF77 and GhERF88 in root development, GhERF139D in leaf development and GhRAV1 in fiber elongation (Fig. 5). The genes involved in different stresses, tissues and developmental stages provide fine candidates for cotton improvement. Zhou et al. [60] identified GhERF12 in G. barbadense, which is involved in cotton seedling growth and development and induced by 1-aminocyclopropane-1carboxylic acid (ACC) and indole-3-acetic acid (IAA). It is homologous to GhERF76, which was identified in this study in cotton roots, ovules and fibers and may play a role in cotton root and fiber development.

Studies have shown that DREB and ERF genes are responsive to abiotic stress, so we investigated the expression profiles of DREB and ERF genes in G. hirsutum under stress treatments using RNA-seq data and qRT-PCR validation. As expected, a large number of DREB and ERF genes were induced by cold, heat and drought (Figs. 6, 7 and 8). Several DREB and ERF genes were induced by multiple stresses, revealing crosstalk between multiple environmental stimuli. This may provide clues for the studying of crosstalk in different stress conditions. In addition, overexpression of ERF genes resulted in improved tolerance to drought and salt in Egyptian clover [61], osmotic stress in tobacco [62], and cold, drought and heat responses in Arabidopsis [63]. These genes in cotton were induced by cold, heat or drought (Fig. 6), suggesting widespread and conserved tolerance to stress via DREB and ERF genes in different plant species. Of the 28 selected genes, 17 GhDREB and GhERF genes were induced by both cold and drought (Fig. 7). Genome wide analysis of AP2/EREBP genes will provided candidates for cotton stress tolerance improvement.

The fates of cotton homoeologous genes

Duplicated or homoeologous genes in plants experienced different fates, including non-functionalization, neo-functionalization or partitioned aggregate ancestral functions (sub-functionalization) [64]. The expression profiles of the tissues examined in this study provide examples of the functional divergence of AP2/EREBP genes. As shown in Additional file 12: Figure S8, the expression levels of members of the AP2/EREBP family in G. hirsutum differed in the different tissues. Additionally, almost half of the tandem duplicated genes were not expressed (Additional file 13: Figure S9), indicating functional redundancy of tandem duplicated genes in allotetraploid cotton. Although there was no direct molecular evidence for the functional divergence of homoeologous gene pairs, the results of this study provide a whole genome information for research into gene fates in G. hirsutum during evolution and domestication.

Conclusions

We performed the first genome-wide analysis of the AP2/EREBP family genes in cotton and conducted a detailed investigation of their classification, structure, gene expansion, synteny and expression profiles in different tissues and in response to various abiotic stresses. Results revealed that the AP2/EREBP genes were obviously expanded in Gossypium mainly by segmental duplication except for the B3 group of ERF subfamily mainly by tandem duplication. The GhDREB and GhERF genes play crucial roles in cotton stress responses. To the best of our knowledge, our data provide insights into characteristics and potential functions of cotton AP2/EREBPs. The results provide a useful basis for further research into the structure, function and phylogenetic relationships of these gene family members. This will help the identification of excellent candidate genes for genetic engineering to improve stress tolerance and developmental research in cotton and other valuable plants.

Methods

Identification and chromosomal mapping

The G. raimondii, A. thaliana, V. vinifera, and T. cacao gene files were downloaded from Phytozome v11.0 (http://www.phytozome.net/). The gene information of G. hirsutum acc. TM-1 was downloaded from http://mascotton.njau.edu.cn . The Hidden Markov Model (HMM) profile of the AP2 domain (PF00847) was obtained from the Pfam website (http://pfam.xfam.org/), and was employed as a query to identify all possible AP2/EREBPs using HMMER (V3.0) software [65]. To validate the HMM search, all candidate sequences were used as queries to search the NCBI non-redundant (nr) protein database with the blastp program, and the results with the best ‘AP2’ hits were retained for further analysis. To predict the GPI-anchor attachment sites, the BGI-PI47 [66] and GPI-SOM48 [67] algorithms were used. The AP2/ERF sequences were confirmed based on the presence of an AP2 domain, and all of the putative AP2/ERF proteins were aligned to Arabidopsis AP2/ERF proteins to classify them into different groups, as described by Feng et al. [20].

Positional information on all of the AP2/EREBPs was parsed from the General Feature Format (GFF) files downloaded from Phytozome v11.0, and the locations of AP2/EREBPs in G. raimondii and G. hirsutum were drafted using MapInspect software (http://mapinspect.software.informer.com/).

Phylogenetic and gene structure analysis

A multiple alignment of the sequences encoding the conserved AP2/ERF domain was constructed with ClustalX (version 2.0) [68], and the gaps and poorly aligned sections were removed. A phylogenetic tree was generated using the maximum likelihood method and WAG model in MEGA v5.2 [69] software, and the reliability of interior branches was assessed with 1000 bootstrap resamplings.

The gene structures of the AP2/EREBPs were parsed from the General Feature Format (GFF) files, and diagrams of the exon-intron structures were drawn using the online program Gene Structure Display Server (GSDS; http://gsds.cbi.pku.edu.cn/).

Gene duplication and synteny analysis

The syntenic information of G. raimondii, A. thaliana, V. vinifera and T. cacao was downloaded from the Plant Genome Duplication Database (PGDD; http://chibba.agtec.uga.edu/duplication/). AP2/EREBPs were mapped to the syntenic blocks for intra- and inter-genomic comparison. A syntenic diagram was drawn using Circos software [70].

The timing of segmental duplication events can be estimated by computing mean Ks values for all anchor points located in the corresponding syntenic block [43, 47], and all the Ks values were parsed from PGDD syntenic data. Genes separated by five or fewer genes within a 100-kb region on a chromosome may have resulted from tandem duplication [71].

Plant materials and stress treatments

The widely used genetic standard line, G. hirsutum acc. Texas Marker-1 (TM-1), which is not conflict to any permissions or licences, was used for tissue/organ expression analysis. Roots, stems and leaves were collected from 2-week-old seedlings grown in a growth chamber. Petals, anthers and ovules were collected from plants grown under standard field conditions on the day of flowering, and fibers were excised from developing bolls on selected days post anthesis (dpa). True leaves of the seedlings were treated with PEG, heat and cold. All the RNA-Seq data were taken from Zhang et al. [42].

G. hirsutum acc. TM-1 was used to validate the expression profiles of the AP2/EREBPs under stress treatments. Cotton seedlings were grown in a growth chamber with fixed chamber condition (light/dark cycle: 14 h at 28 °C/10 h at 25 °C; 70% relative humidity). Three-week-old seedlings were treated as follows: for the drought stress, the roots of cotton seedlings were irrigated with 20% PEG), and for the temperature stress, seedlings were placed in a growth chamber at a low temperature conditions (4 °C). Seedlings grown in normal conditions were used as a mock control. The first two true leaves were collected at 0 h (just before stress treatment), and 1, 3, 6, 12 and 24 h after stress treatment,and immediately frozen in liquid nitrogen and stored at −70 °C. The experiments were repeated three times, each with 18 plants per treatment and standard errors were from the means of three biological replicates.

RNA isolation and quantitative reverse transcription PCR

Total RNA was isolated using a plant RNA purification kit (MoLFarming, Cat.No. RK16-50 T, China) from leaf tissues according to the manufacturer's instructions. T-Test was used for statistical analysis. The expression of GhDREB/ERFs was analyzed using an ABI 7500 real-time PCR system with the SYBR Green Master Mix (Vazyme, Nanjing, China). Gene-specific primers were designed based on the GhDREB/ERF gene sequences using Primer Premier 5.0. Cotton histone3 (AF024716) was used as the reference gene [72]. The amplification parameters were as follows: 95 °C hold for 10 min, followed by 40 cycles at 95 °C for 15 s, 58 °C for 15 s and 72 °C for 15 s. For the melting curve stage, the default settings were chosen. Nonspecific products were identified by inspecting melting curves. All the primers used in this paper have been listed in an additional table: Additional file 14: Table S5.

Investigation of the expressions pattern of GhAP2/EREBP genes

Expression data for GhAP2/EREBP genes was obtained from the transcriptome data [42]. These datasets correspond to gene expression intensities in various tissues and under abiotic stresses. Gene expression levels in the different tissues were calculated according to FPKM values and the default empirical abundance threshold of FPKM > 1 was used to identify the expressed genes. In abiotically stressed plants, genes with expression levels (FPKM) twofold or more greater than in controls were identified as up-regulated genes, and genes with expression levels that were less than one half of that in controls were identified as down-regulated. Expression patterns were clustered by Mev4.6.2 software using the Hierarchical Clustering model (http://www.tm4.org/mev.html).

Abbreviations

AP2:

APETALA2

AP2/EREBP:

APETALA2/ethylene-responsive element binding protein

DREB:

Dehydration-Responsive Element Binding protein

ERF:

Ethylene-Responsive Factor

Gh:

Gossypium hirsutum

Gr:

Gossypium raimondii

PGDD:

Plant Genome Duplication Database

qRT-PCR:

Quantitative reverse transcription PCR.

RAV:

Related to ABI3/VP1

WGD:

Whole-genome duplication

References

  1. Jofuku KD, den Boer BG, Van Montagu M, Okamuro JK. Control of Arabidopsis flower and seed development by the homeotic gene APETALA2. Plant Cell. 1994;6(9):1211–25.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  2. Ohme-Takagi M, Shinshi H. Ethylene-inducible DNA-binding proteins that interact with an ethylene responsive element. Plant Cell. 1995;7(2):173–82.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  3. Wessler SR. Homing into the origin of the AP2 DNA binding domain. Trends Plant Sci. 2005;10(2):54–6.

    CAS  Article  PubMed  Google Scholar 

  4. Dubouzet JG, Sakuma Y, Ito Y, Kasuga M, Dubouzet EG, Miura S, et al. OsDREB genes in rice, Oryza sativa L., encode transcription activators that function in drought-, high-salt- and cold-responsive gene expression. Plant J. 2003;33(4):751–63.

    CAS  Article  PubMed  Google Scholar 

  5. Shinozaki K, Yamaguchi-Shinozaki K, Seki M. Regulatory network of gene expression in the drought and cold stress responses. Curr Opin Plant Biol. 2003;6:410–7.

    CAS  Article  PubMed  Google Scholar 

  6. Gutterson N, Reuber TL. Regulation of disease resistance pathways by AP2/ERF transcription factors. Curr Opin Plant Biol. 2004;7(4):465–71.

    CAS  Article  PubMed  Google Scholar 

  7. Matías-Hernández L, Aguilar-Jaramillo AE, Marín-González E, Suárez-López P, Pelaz S. RAV genes: regulation of floral induction and beyond. Ann Bot. 2014;114(7):1459–70.

    Article  PubMed  PubMed Central  Google Scholar 

  8. Rashotte AM, Goertzen LR. The CRF domain defines cytokinin response factor proteins in plants. BMC Plant Biol. 2010;10:74.

    Article  PubMed  PubMed Central  Google Scholar 

  9. Shen YG, Zhang WK, He SJ, Zhang JS, Liu Q, Chen SY. An EREBP/AP2-type protein in Triticum aestivum was a DRE-binding transcription factor induced by cold, dehydration and ABA stress. Theor Appl Genet. 2003;106(5):923–30.

    CAS  Article  PubMed  Google Scholar 

  10. Hu Y, Jiang L, Wang F, Yu D. Jasmonate regulates the inducer of cbf expression-C-repeat binding factor/DRE binding factor1 cascade and freezing tolerance in Arabidopsis. Plant Cell. 2013;25(8):2907–24.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  11. Dietz KJ, Vogel MO, Viehhauser A. AP2/EREBP transcription factors are part of gene regulatory networks and integrate metabolic, hormonal and environmental signals in stress acclimation and retrograde signalling. Protoplasma. 2010;245(1–4):3–14.

    CAS  Article  PubMed  Google Scholar 

  12. Zhuang J, Cai B, Peng RH, Zhu B, Jin XF, Xue Y, et al. Genome-wide analysis of the AP2/ERF gene family in Populus trichocarpa. Biochem Biophys Res Commun. 2008;371(3):468–74.

    CAS  Article  PubMed  Google Scholar 

  13. Zhang G, Chen M, Chen X, Xu Z, Guan S, Li LC, et al. Phylogeny, gene structures, and expression patterns of the ERF gene family in soybean (Glycine max L.). J Exp Bot. 2008;59(15):4095–107.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  14. Sharma MK, Kumar R, Solanke AU, Sharma R, Tyagi AK, Sharma AK. Identification, phylogeny, and transcript profiling of ERF family genes during development and abiotic stress treatments in tomato. Mol Genet Genomics. 2010;284(6):455–75.

    CAS  Article  PubMed  Google Scholar 

  15. Sharoni AM, Nuruzzaman M, Satoh K, Shimizu T, Kondoh H, Sasaya T, et al. Gene structures, classification and expression models of the AP2/EREBP transcription factor family in rice. Plant Cell Physiol. 2011;52(2):344–60.

    CAS  Article  PubMed  Google Scholar 

  16. Charfeddine M, Saïdi MN, Charfeddine S, Hammami A, Gargouri BR. Genome-wide analysis and expression profiling of the ERF transcription factor family in potato (Solanum tuberosum L.). Mol Biotechnol. 2014;57(4):348–58.

    Article  Google Scholar 

  17. Shu Y, Liu Y, Zhang J, Song L, Guo C. Genome-wide analysis of the AP2/ERF superfamily genes and their responses to abiotic stress in Medicago truncatula. Front Plant Sci. 2015;6:1247.

    PubMed  Google Scholar 

  18. Chen L, Han J, Deng X, Tan S, Li L, Li L, et al. Expansion and stress responses of AP2/EREBP superfamily in Brachypodium Distachyon. Sci Rep. 2016;6:21623.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  19. Allen MD, Yamasaki K, Ohme-Takagi M, Tateno M, Suzuki M. A novel mode of DNA recognition by a beta-sheet revealed by the solution structure of the GCC-box binding domain in complex with DNA. EMBO J. 1998;17(18):5484–96.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  20. Feng JX, Liu D, Pan Y, Gong W, Ma LG, Luo JC, et al. An annotation update via cDNA sequence analysis and comprehensive profiling of developmental, hormonal or environmental responsiveness of the Arabidopsis AP2/EREBP transcription factor gene family. Plant Mol Biol. 2005;59(6):853–68.

    CAS  Article  PubMed  Google Scholar 

  21. Okamuro JK, Caster B, Villarroel R, Van Montagu M, Jofuku KD. The AP2 domain of APETALA2 defines a large new family of DNA binding proteins in Arabidopsis. Proc Natl Acad Sci U S A. 1997;94(13):7076–81.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  22. Krizek B. AINTEGUMENTA and AINTEGUMENTA-LIKE6 act redundantly to regulate Arabidopsis floral growth and patterning. Plant Physiol. 2009;150(4):1916–29.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  23. Chung MY, Vrebalov J, Alba R, Lee J, McQuinn R, Chung JD, et al. A tomato (Solanum lycopersicum) APETALA2/ERF gene, SlAP2a, is a negative regulator of fruit ripening. Plant J. 2010;64(6):936–47.

    CAS  Article  PubMed  Google Scholar 

  24. Licausi F, Giorgi FM, Zenoni S, Osti F, Pezzotti M, Perata P. Genomic and transcriptomic analysis of the AP2/ERF superfamily in Vitis vinifera. BMC Genomics. 2010;11:719.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  25. Dinh TT, Girke T, Liu X, Yant L, Schmid M, Chen X. The floral homeotic protein APETALA2 recognizes and acts through an AT-rich sequence element. Development. 2012;139(11):1978–86.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  26. Tang Y, Qin S, Guo Y, Chen Y, Wu P, Chen Y, et al. Genome-wide analysis of the AP2/ERF gene family in physic nut and overexpression of the JcERF011 gene in rice increased its sensitivity to salinity stress. PLoS One. 2016;11(3):e0150879.

    Article  PubMed  PubMed Central  Google Scholar 

  27. Giraudat J, Hauge BM, Valon C, Smalle J, Parcy F, Goodman HM. Isolation of the Arabidopsis ABI3 gene by positional cloning. Plant Cell. 1992;4(10):1251–61.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  28. Mizoi J, Shinozaki K, Yamaguchi-Shinozaki K. AP2/ERF family transcription factors in plant abiotic stress responses. Biochim Biophys Acta. 2012;1819(2):86–96.

    CAS  Article  PubMed  Google Scholar 

  29. Alonso JM, Stepanova AN, Leisse TJ, Kim CJ, Chen H, Shinn P, et al. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science. 2003;301(5633):653–7.

    Article  PubMed  Google Scholar 

  30. Hu YX, Wang YX, Liu XF, Li JY. Arabidopsis RAV1 is down-regulated by brassinosteroid and may act as a negative regulator during plant development. Cell Res. 2004;14(1):8–15.

    CAS  Article  PubMed  Google Scholar 

  31. Sohn KH, Lee SC, Jung HW, Hong JK, Hwang BK. Expression and functional roles of the pepper pathogen-induced transcription factor RAV1 in bacterial disease resistance, and drought and salt stress tolerance. Plant Mol Biol. 2006;61(6):897–915.

    CAS  Article  PubMed  Google Scholar 

  32. Li CW, Su RC, Cheng CP, Sanjaya, You SJ, Hsieh TH, et al. Tomato RAV transcription factor is a pivotal modulator involved in the AP2/EREBP-mediated defense pathway. Plant Physiol. 2011;33(156):213–27.

    CAS  Article  Google Scholar 

  33. Sakuma Y, Liu Q, Dubouzet JG, Abe H, Shinozaki K, Yamaguchi-Shinozaki K. DNA-binding specificity of the ERF/AP2 domain of Arabidopsis DREBs, transcription factors involved in dehydration- and cold-inducible gene expression. Biochem Biophys Res Commun. 2002;290(3):998–1009.

    CAS  Article  PubMed  Google Scholar 

  34. Fujimoto SY, Ohta M, Usui A, Shinshi H, Ohme-Takagi M. Arabidopsis ethylene-responsive element binding factors act as transcriptional activators or repressors of GCC box-mediated gene expression. Plant Cell. 2000;12(3):393–404.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  35. Onãte-Sánchez L, Singh KB. Identification of Arabidopsis ethylene-responsive element binding factors with distinct induction kinetics after pathogen infection. Plant Physiol. 2002;128(128):1313–22.

    Article  PubMed  PubMed Central  Google Scholar 

  36. Mantiri FR, Kurdyukov S, Lohar DP, Sharopova N, Saeed NA, Wang XD, et al. The transcription factor MtSERF1 of the ERF subfamily identified by transcriptional profiling is required for somatic embryogenesis induced by auxin plus cytokinin in Medicago truncatula. Plant Physiol. 2008;146(4):1622–36.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  37. Cai XT, Xu P, Zhao PX, Liu R, Yu LH, Xiang CB. Arabidopsis ERF109 mediates cross-talk between jasmonic acid and auxin biosynthesis during lateral root formation. Nat Commun. 2014;5:5833.

    CAS  Article  PubMed  Google Scholar 

  38. Cao ZF, Li J, Chen F, Li YQ, Zhou HM, Liu Q. Effect of two conserved amino acid residues on DREB1A function. Biochemistry (Mosc). 2001;66(6):623–7.

    CAS  Article  Google Scholar 

  39. Xu ZS, Chen M, Li LC, Ma YZ. Functions and application of the AP2/ERF transcription factor family in crop improvement. J Integr Plant Biol. 2011;53(7):570–85.

    CAS  Article  PubMed  Google Scholar 

  40. Yamaguchi-Shinozaki K, Shinozaki K. A novel cis-acting element in an Arabidopsis gene is involved in responsiveness to drought, low-temperature, or high-salt stress. Plant Cell. 1994;6(2):251–64.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  41. Huang GT, Ma SL, Bai LP, Zhang L, Ma H, Jia P, et al. Signal transduction during cold, salt, and drought stresses in plants. Mol Biol Rep. 2012;39(2):969–87.

    Article  PubMed  Google Scholar 

  42. Zhang T, Hu Y, Jiang W, Fang L, Guan X, Chen J, et al. Sequencing of allotetraploid cotton (Gossypium hirsutum L. acc. TM-1) provides a resource for fiber improvement. Nat Biotechnol. 2015;33(5):531–7.

    CAS  Article  PubMed  Google Scholar 

  43. Paterson AH, Wendel JF, Gundlach H, Guo H, Jenkins J, Jin D, et al. Repeated polyploidization of Gossypium genomes and the evolution of spinnable cotton fibres. Nature. 2012;492(7429):423–7.

    CAS  Article  PubMed  Google Scholar 

  44. Zhang Y, Mao L, Wang H, Brocker C, Yin X, Vasiliou V, et al. Genome-wide identification and analysis of grape aldehyde dehydrogenase (ALDH) gene superfamily. PLoS One. 2012;7(2), e32153.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  45. Rizzon C, Ponger L, Gaut BS. Striking similarities in the genomic distribution of tandemly arrayed genes in Arabidopsis and rice. PLoS Comput Biol. 2006;2(9), e115.

    Article  PubMed  PubMed Central  Google Scholar 

  46. Hanada K, Zou C, Lehti-Shiu MD, Shinozaki K, Shiu SH. Importance of lineage-specific expansion of plant tandem duplicates in the adaptive response to environmental stimuli. Plant Physiol. 2008;148(2):993–1003.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  47. Wang K, Wang Z, Li F, Ye W, Wang J, Song G, et al. The draft genome of a diploid cotton Gossypium raimondii. Nat Genet. 2012;44(10):1098–103.

    CAS  Article  PubMed  Google Scholar 

  48. Tang H, Wang X, Bowers JE, Ming R, Alam M, Paterson AH. Unraveling ancient hexaploidy through multiple-aligned angiosperm gene maps. Genome Res. 2008;18(12):1944–54.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  49. Fawcett JA, Maere S, Van de Peer Y. Plants with double genomes might have had a better chance to survive the Cretaceous-Tertiary extinction event. Proc Natl Acad Sci U S A. 2009;106(14):5737–42.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  50. Van de Peer Y, Fawcett JA, Proost S, Sterck L, Vandepoele K. The flowering world: a tale of duplications. Trends Plant Sci. 2009;14(12):680–8.

    Article  PubMed  Google Scholar 

  51. Kitomi Y, Ito H, Hobo T, Aya K, Kitano H, Inukai Y. The auxin responsive AP2/ERF transcription factor CROWN ROOTLESS5 is involved in crown root initiation in rice through the induction of OsRR1, a type-A response regulator of cytokinin signaling. Plant J. 2011;67(3):472–84.

    CAS  Article  PubMed  Google Scholar 

  52. Kunst L, Klenz JE, Martinez-Zapater J, Haughn GW. AP2 Gene determines the identity of perianth organs in flowers of Arabidopsis thaliana. Plant Cell. 1989;1(12):1195–208.

    Article  PubMed  PubMed Central  Google Scholar 

  53. McCarty DR, Hattori T, Carson CB, Vasil V, Lazar M, Vasil IK. The Viviparous-1 developmental gene of maize encodes a novel transcriptional activator. Cell. 1991;66(5):895–905.

    CAS  Article  PubMed  Google Scholar 

  54. Ulmasov T, Hagen G, Gulfoyle TJ. ARF1, a transcription factor that binds to auxin response elements. Science. 1997;276(5320):1865–8.

    CAS  Article  PubMed  Google Scholar 

  55. Liu Q, Kasuga M, Sakuma Y, Abe H, Miura S, Yamaguchi-Shinozaki K, et al. Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and lowtemperature-responsive gene expression, respectively, in Arabidopsis. Plant Cell. 1998;10(8):1391–406.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  56. Lynch M, Conery JS. The evolutionary fate and consequences of duplicate genes. Science. 2000;290(5494):1151–5.

    CAS  Article  PubMed  Google Scholar 

  57. Osborn TC, Pires JC, Birchler JA, Auger DL, Chen ZJ, Lee HS, et al. Understanding mechanisms of novel gene expression in polyploids. Trends Genet. 2003;19(3):141–7.

    CAS  Article  PubMed  Google Scholar 

  58. Riddle NC, Birchler JA. Effects of reunited diverged regulatory hierarchies in allopolyploids and species hybrids. Trends Genet. 2003;19(11):597–600.

    CAS  Article  PubMed  Google Scholar 

  59. Jiao Y, Wickett NJ, Ayyampalayam S, Chanderbali AS, Landherr L, Ralph PE, et al. Ancestral polyploidy in seed plants and angiosperms. Nature. 2011;473(7345):97–100.

    CAS  Article  PubMed  Google Scholar 

  60. Zhou Y, Xia H, Li XJ, Hu R, Chen Y, Li XB. Overexpression of a cotton gene that encodes a putative transcription factor of AP2/EREBP family in Arabidopsis affects growth and development of transgenic plants. PLoS One. 2013;8(10), e78635.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  61. Abogadallah GM, Nada RM, Malinowski R, Quick P. Overexpression of HARDY, an AP2/ERF gene from Arabidopsis, improves drought and salt tolerance by reducing transpiration and sodium uptake in transgenic Trifolium alexandrinum L. Planta. 2011;233(6):1265–76.

    CAS  Article  PubMed  Google Scholar 

  62. Park JM, Park CJ, Lee SB, Ham BK, Shin R, Paek KH. Over expression of the tobacco Tsi1 gene encoding an EREBP/AP2-type transcription factor enhances resistance against pathogen attack and osmotic stress in tobacco. Plant Cell. 2001;13(5):1035–46.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  63. Kang HG, Kim J, Kim B, Jeong H, Choi SH, Kim EK, et al. Overexpression of FTL1/DDF1, an AP2 transcription factor, enhances tolerance to cold, drought, and heat stresses in Arabidopsis thaliana. Plant Sci. 2011;180(4):634–41.

    CAS  Article  PubMed  Google Scholar 

  64. Roulin A, Auer PL, Libault M, Schlueter J, Farmer A, May G, et al. The fate of duplicated genes in a polyploid plant genome. Plant J. 2013;73(1):143–53.

    CAS  Article  PubMed  Google Scholar 

  65. Finn RD, Clements J, Eddy SR. HMMER web server: interactive sequence similarity searching. Nucleic Acids Res. 2011;39:W29–37.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  66. Eisenhaber B, Wildpaner M, Schultz CJ, Borner GH, Dupree P, Eisenhaber F. Glycosylphosphatidylinositol lipid anchoring of plant proteins. Sensitive prediction from sequence- and genome-wide studies for Arabidopsis and rice. Plant Physiol. 2003;133(4):1691–701.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  67. Fankhauser N, Maser P. Identification of GPI anchor attachment signals by a Kohonen self-organizing map. Bioinformatics. 2005;21(9):1846–52.

    CAS  Article  PubMed  Google Scholar 

  68. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, et al. Clustal W and Clustal X version 2.0. Bioinformatics. 2007;23(21):2947–8.

    CAS  Article  PubMed  Google Scholar 

  69. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 2011;28(10):2731–9.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  70. Krzywinski M, Schein J, Birol I, Connors J, Gascoyne R, Horsman D, et al. Circos: an information aesthetic for comparative genomics. Genome Res. 2009;19(9):1639–45.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  71. Wang L, Guo K, Li Y, Tu Y, Hu H, Wang B, et al. Expression profiling and integrative analysis of the CESA/CSL superfamily in rice. BMC Plant Biol. 2010;10:282.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  72. Xu YH, Wang JW, Wang S, Wang JY, Chen XY. Characterization of GaWRKY1, a cotton transcription factor that regulates the sesquiterpene synthase gene (+)-delta-cadinene synthase-A. Plant Physiol. 2004;135(1):507–15.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

We thank Xiaoyang Xu of the Cotton Research Institute of Nanjing Agricultural University for providing valuable advice on bioinformatics analysis.

Funding

National Research and Development Project of Transgenic Crops of China (2016ZX08009-003)‚ the Major State Research Development Program of China (2016YFD0101001) and the Priority Academic Program Development of Jiangsu Higher Education Institutions and the JCIC-MCP project.

Availability of data and materials

The transcriptome and genome data supporting the conclusions of this article are from Zhang et al. 2015, with the accession codes, SRA: PRJNA248163.

Authors’ contributions

CXL conceived the idea, design the experiment, analyzed the data and drafted the manuscript; TZZ conceptualized the research program and revised the manuscript. All authors have read and approved the final version of this manuscript.

Competing interests

The authors declare that they have no competing interests.

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Not applicable.

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetics & Germplasm Enhancement, Cotton Research Institute, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, People’s Republic of China

    Chunxiao Liu & Tianzhen Zhang

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  1. Chunxiao Liu
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  2. Tianzhen Zhang
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Correspondence to Tianzhen Zhang.

Additional files

Additional file 1: Table S1.

AP2/EREBP superfamily genes in G. raimondii. (XLS 46 kb)

Additional file 2: Table S2.

AP2/EREBP superfamily genes in G. hirsutum acc. TM-1. (XLS 77 kb)

Additional file 3: Figure S1.

Gene clustering of the AP2/EREBP superfamily homologous genes in G. raimondii and G. hirsutum. Maximum likelihood method was used to the sequence alignment and phylogeny tree construction. (PDF 950 kb)

Additional file 4: Figure S2.

Comparison of deduced amino acid sequences of the DREB and ERF conserved domains. The black background represents conserved amino acid residues in each group. (TIF 1648 kb)

Additional file 5: Figure S3.

Phylogeny tree of the AP2/EREBP superfamily genes in G. raimondii and A. thaliana. (TIF 1028 kb)

Additional file 6: Table S3.

Tandem duplicated genes of AP2/EREBP superfamily genes in G. raimondii. (XLS 33 kb)

Additional file 7: Figure S4.

Gene structures of GrAP2/EREBP genes. Exons and introns are represented by blue boxes and black lines, respectively, and their sizes are indicated by the scale at the top. (TIF 477 kb)

Additional file 8: Table S4.

Ks values for segmental duplicated gene pairs of AP2/EREBP superfamily genes in G. raimondii. (XLS 60 kb)

Additional file 9: Figure S5.

Synteny comparison of AP2/EREBP subfamily of G. raimondii to cacao (a) and grape (b). (TIF 4306 kb)

Additional file 10: Figure S6.

Comparison number of GrDREB, GrERF, GrAP2 and GrRAV genes among G. raimondii, T. cacao and V. vinifera. (TIF 58 kb)

Additional file 11: Figure S7.

Venn diagram of transcripts identified in cotton under different abiotic stress conditions. Cold, drought and heat. (TIF 48 kb)

Additional file 12: Figure S8.

Comparisons of expression profiles of nine representative homoeologous gene pairs of AP2/EREBP family of G. hirsutum in various tissues. Represented in y-axes are the FPKM levels of the RNA-seq data [42] and the x-axes are ten representative tissues. (TIF 189 kb)

Additional file 13: Figure S9.

Expression profiles (in log2 based fold change) of tandem duplicated genes in G. hirsutum TM-1. Each block indicates one tandem duplicated gene cluster. The scale bars represent log2 of the RPKM values. (TIF 323 kb)

Additional file 14: Table S5.

All the primers used in this paper. (XLSX 10 kb)

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Liu, C., Zhang, T. Expansion and stress responses of the AP2/EREBP superfamily in cotton. BMC Genomics 18, 118 (2017). https://doi.org/10.1186/s12864-017-3517-9

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Keywords

  • Gossypium
  • Polyploid
  • Duplicated genes
  • Homoeologous genes
  • Gene expansion
  • Stress response

BMC Genomics

ISSN: 1471-2164

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