Fig. 6

qRT-PCR analyses of genes that are specifically or preferentially induced by glutamate. 17-day-old rice seedlings grown in hydroponics without nitrogen were subsequently transferred to medium containing 2.5 mM glutamate, glutamine, or 1.43 mM NH₄NO₃ for 0, 15 min, 30 min, 1, 4, and 24 h. Total RNA extracted from roots was used for qRT-PCR to analyze the expression of Os08g0508800 (herbivore induced 13-lipoxygenase, HI-LOX), Os01g0666000 (lipid phosphate phosphatase 2, LPP2), Os10g0542900 (chitinase 8, CHT8), Os09g0471800 (wall associated kinase 85, WAK85), Os03g0203700 (calcium transporting ATPase 2, CaATP2), Os12g0198200 (phosphatase 2C, PP2C) (a), Os12g0518200 (EamA-like transporter), Os01g0905300 (exocyst 70 subunit, EXO70) (b), and four unknown function genes Os04g0618400, Os01g0952900, Os03g0302800 and Os12g0248600 (c). The number of each gene corresponds to the number in Table 1. Relative expression indicates the fold-change of each gene as compared to that of control