Table 3 Carbon fixation proteins from the hydroxypropionate–hydroxybutyrate carbon fixation pathway in M. sedula and the homologous found in A. copahuensis genome
| Â | Metallosphaera sedula | Acidianus copahuensis | Â | |||||
|---|---|---|---|---|---|---|---|---|
Gene name | Uniprot ID | ORF (aa) | NCBI accession | ORF (aa) | E-value | Aa identical positions (%) | HhcR (position) | Protein Function |
HhcR | A4YIR2 | 115 | EZQ01893 | 115 | 1.00E-46 | 64 | Â | HhcR transcription factor |
accA | A4YD22 | 510 | EZQ11106 | 507 | 0 | 78 | + (−46) | Acetyl-CoA/propionyl-CoA carboxylase, alpha subunit |
accB | A4YD23 | 167 | EZQ11107 | 167 | 2.00E-84 | 64 | - | Acetyl-CoA/propionyl-CoA carboxylase, beta subunit |
accC | A4YGI1 | 524 | EZQ11108 | 523 | 0 | 81 | + (−53) | Acetyl-CoA/propionyl-CoA carboxylase, gamma subunit |
mcr | A4YEN2 | 357 | EZQ11004 | 356 | 0 | 82 | ? (−274) | Malonyl-CoA/succinyl-CoA reductase |
msr | A4YI81 | 314 | EZQ04857 | 317 | 2.00E-174 | 75 | + (−20) | Malonate semialdehyde reductase |
hpcS | A4YGR1 | 661 | EZQ11066 | 653 | 0 | 78 | - | 3-Hydroxypropionyl-CoA synthetase |
acr | A4YGN2 | 332 | EZQ01730 | 333 | 0 | 76 | + (−19) | Acryloyl-CoA reductase |
mce | A4YEG2 | 140 | EZQ10835 | 141 | 4.00E-71 | 70 | + (−65) | Methylmalonyl-CoA epimerase |
mcmA | A4YEG1 | 553 | EZQ10834 | 550 | 0 | 83 | ? (−242) | Methylmalonyl-CoA mutase (catalytic subunit) |
mcmB | A4YIE3 | 155 | EZQ04694 | 138 | 8.00E-75 | 78 | + (−39) | Methylmalonyl-CoA mutase (coenzyme B12-binding subunit) |
ssr | A4YGN0 | 360 | EZQ01731 | 360 | 0 | 77 | + (−41) | Succinate semialdehyde reductase |
hbcS1 | A4YDR9 | 549 | - | - | - | - | - | 4-Hydroxybutyryl-CoA synthetase |
hbcS2 | A4YDT1 | 564 | EZQ11368 | 559 | 0 | 58 | - | 4-Hydroxybutyryl-CoA synthetase |
hbcS3 | A4YGM8 | 472 | EZQ01733 | 471 | 0 | 85 | + (−37) | 4-Hydroxybutyryl-CoA synthetase |
hbcD | A4YGC7 | 507 | EZQ02033 | 506 | 0 | 75 | + (−30) | 4-Hydroxybutyryl-CoA dehydratase |
ack | A4YEH9 | 395 | EZQ11076 | 396 | 0 | 71 | + (−40) | Acetoacetyl-CoA-ketothiolase |
hbd2 | A4YGM9 | 391 | EZQ01732 | 388 | 0 | 72 | + (−46) | Probable 3-hydroxybutyryl-CoA dehydrogenase |
hpcD-hbd | A4YDS4 | 651 | EZQ11226 | 661 | 0 | 49 | + (−91) | Bifunctional crotonyl-CoA hydratase/3-hydroxybutyryl-CoA dehydrogenase |
|  | A4YI89 | 259 | EZQ04864 | 257 | 2.00E-133 | 71 | + (−15) | 3-hydroxypropionyl-coenzyme A dehydratase |