Genome analysis of the thermoacidophilic archaeon Acidianus copahuensis focusing on the metabolisms associated to biomining activities

María Sofía Urbieta; Nicolás Rascovan; Martín P. Vázquez; Edgardo Donati

doi:10.1186/s12864-017-3828-x

Table 3 Carbon fixation proteins from the hydroxypropionate–hydroxybutyrate carbon fixation pathway in M. sedula and the homologous found in A. copahuensis genome

From: Genome analysis of the thermoacidophilic archaeon Acidianus copahuensis focusing on the metabolisms associated to biomining activities

	Metallosphaera sedula		Acidianus copahuensis
Gene name	Uniprot ID	ORF (aa)	NCBI accession	ORF (aa)	E-value	Aa identical positions (%)	HhcR (position)	Protein Function
HhcR	A4YIR2	115	EZQ01893	115	1.00E^-46	64		HhcR transcription factor
accA	A4YD22	510	EZQ11106	507	0	78	+ (−46)	Acetyl-CoA/propionyl-CoA carboxylase, alpha subunit
accB	A4YD23	167	EZQ11107	167	2.00E^-84	64	-	Acetyl-CoA/propionyl-CoA carboxylase, beta subunit
accC	A4YGI1	524	EZQ11108	523	0	81	+ (−53)	Acetyl-CoA/propionyl-CoA carboxylase, gamma subunit
mcr	A4YEN2	357	EZQ11004	356	0	82	? (−274)	Malonyl-CoA/succinyl-CoA reductase
msr	A4YI81	314	EZQ04857	317	2.00E^-174	75	+ (−20)	Malonate semialdehyde reductase
hpcS	A4YGR1	661	EZQ11066	653	0	78	-	3-Hydroxypropionyl-CoA synthetase
acr	A4YGN2	332	EZQ01730	333	0	76	+ (−19)	Acryloyl-CoA reductase
mce	A4YEG2	140	EZQ10835	141	4.00E^-71	70	+ (−65)	Methylmalonyl-CoA epimerase
mcmA	A4YEG1	553	EZQ10834	550	0	83	? (−242)	Methylmalonyl-CoA mutase (catalytic subunit)
mcmB	A4YIE3	155	EZQ04694	138	8.00E^-75	78	+ (−39)	Methylmalonyl-CoA mutase (coenzyme B12-binding subunit)
ssr	A4YGN0	360	EZQ01731	360	0	77	+ (−41)	Succinate semialdehyde reductase
hbcS1	A4YDR9	549	-	-	-	-	-	4-Hydroxybutyryl-CoA synthetase
hbcS2	A4YDT1	564	EZQ11368	559	0	58	-	4-Hydroxybutyryl-CoA synthetase
hbcS3	A4YGM8	472	EZQ01733	471	0	85	+ (−37)	4-Hydroxybutyryl-CoA synthetase
hbcD	A4YGC7	507	EZQ02033	506	0	75	+ (−30)	4-Hydroxybutyryl-CoA dehydratase
ack	A4YEH9	395	EZQ11076	396	0	71	+ (−40)	Acetoacetyl-CoA-ketothiolase
hbd2	A4YGM9	391	EZQ01732	388	0	72	+ (−46)	Probable 3-hydroxybutyryl-CoA dehydrogenase
hpcD-hbd	A4YDS4	651	EZQ11226	661	0	49	+ (−91)	Bifunctional crotonyl-CoA hydratase/3-hydroxybutyryl-CoA dehydrogenase
	A4YI89	259	EZQ04864	257	2.00E^-133	71	+ (−15)	3-hydroxypropionyl-coenzyme A dehydratase

The proteins are identified by their NCBI accession number (A. copahuensis) or Uniprot identification number (M. sedula). ORF (aa) indicates length of the open reading frame in amino acids, E-values and amino acid identities were obtained by BLASTP analysis. The presence of the HhcR regulatory motifs in A. copahuensis at the promoter of each gene was determined following the procedures described by Leyn et al. 2015 [53]. Presence is indicated with (+), absence by (−) and cases where it was found more distant than average, with (?), and positions upstream the start codon are indicated in parenthesis. Aa: amino acids

Back to article page