Fig. 4

The ethylene-induced shift of histone acetylation marks is EIN2 dependent. a Heat maps show H3K9Ac, H3K14Ac and H3K23Ac enrichment (IgG normalized reads per kilobase per million mapped reads (RPKM)) in ein2-5 at all genes in the Arabidopsis which along with relative mRNA values. Scatter plot for mRNAs was ranked according to gene expression levels in Col-0 under air treatment. Heat maps were ranked according to their gene expression levels. b-d Scatter plot shows mean histone enrichment of H3K9Ac (b), H3K14Ac (c) and H3K23Ac (d) in air versus ethylene treatment in ein2-5. To generate the graph, histone peaks were normalized by MAnorm [37], and peak-associated genes were ranked according to relative mRNA expression levels and divided into five equal sets. Then the normalized peak enrichments in air and ethylene condition were plotted. Solid black lines in the plots denote the linear regression line