Fig. 1

Pipeline for investigating sRNA-mediated regulation in de-etiolating Arabidopsis seedlings. Four-d-old dark-grown seedlings (W0 h) were exposed to continuous white light (W; 100 μE) for 1, 3, 6, 12 and 24 h; 18–22 million (M) reads per library were acquired. Degradome sequencing for dark- (Dark) and light- (equally pooled from the five light-treated time points) grown seedlings were used for identifying sRNA-mediated cleavage of target mRNA. Colored numbers in parentheses indicate the number of sRNAs/targets passing the corresponding filtering criteria