Fig. 6
miR396 positively regulate photomorphogenesis by suppressing GRF levels. a Illustration of the 35S:MIM396 (MIM396) target mimicry construct. The nucleotides generating a bulge at the miR396 target site is highlighted in red. Minimum free energy (MFE) for MIM396 binding to miR396a and miR396b was calculated by RNAHybrid. The expression of GRF1, GRF2, GRF3 and GRF7 is increased in the two independent MIM396 T4 lines. Data are mean ± SD calculated from three technical replicates. Three biological replicates were performed with similar results. b The MIM396 T4 homozygous lines show long hypocotyl length under W at 50 μE. Data are mean ± SE of hypocotyl length for one representative result. * p < 0.01 by Student’s t-test, n ≥ 30. Three biological replicates were performed with similar results. c The grf1 grf2 grf3 triple mutant and the grf7 single mutant shows shorter hypocotyl than their corresponding wild types, Ws and Col-0, under W and dark conditions, respectively. * p < 0.01 by Student’s t-test, n ≥ 30. Data are one representative result from three biological replicates performed with similar results