Fig. 3

qRT-PCR analysis of LjSGAT genes expression in different tissues of L. japonicus. Plants were grown for 6 weeks and irrigated with Hornum medium (a, c), containing NH₄NO₃ or inoculated with M. loti and irrigated with Hornum medium without nitrogen (b, d). The samples were harvested at 4 h after the beginning of the light or dark period for the quantification of transcripts. Transcript levels are reported as relative units (r.u.). Data are the mean ± SE of three independent biological replicates