Table 1 Strains and plasmids used in this study
Relevant characteristics | Source/Reference | |
|---|---|---|
Strain | ||
HGB800 | Xenorhabdus nematophila isolated from Steinernema carpocapsae All nematodes | ATCC19061 |
HGB1053 | Xenorhabdus bovienii SS-2004 | [119] |
HGB1681 | Xenorhabdus innexi isolated from Steinernema scapterisci nematodes from Grover Smart. Also called Xenorhabdus MT, deposited to ATCC in 2005. | G.C. Smart Jr. University of Florida; ATCC PTA-6826 |
HGB1997 | Xenorhabdus innexi isolated in 2013 from Steinernema scapterisci nematodes obtained from Becker-Underwood | This study |
HGB2171 | HGB1681 attTn7/Tn7-GFP (from pURR25) | This study |
HGB2172 | HGB1997 attTn7/Tn7-GFP (from pURR25) | This study |
HGB283 | Escherichia coli S17–1 lambda pir pUX-BF13 | [107] |
HGB1262 | Escherichia coli BW29427 pURR25, mini Tn7KS-GFP | B. Lies and D. Newman [108] |
TOP10 | E. coli strain for general cloning | Thermo |
Plasmids | ||
pBluescript II SK (−) | General cloning | Stratagene |
pKanWOR | pBluescript KS+ with Km cassette (1 kb) in BamHI site | H. Goodrich-Blair |
pCR-Blunt II-TOPO | General cloning vector, Kanr | Thermo |
pBlueXIS1_460109Up | XIS1_460109Up inserted in pBluescript SK- | This study |
pBlueXIS1_460109UpDn | XIS1_460109Dn inserted in pBlueXIS1_460109Up | This study |
pBlueXIS1_460109UpKanDn | Kan cassette from pKanWOR inserted in pBlueXIS1_460109UpDn | This study |
pBlueXIS1_460115Up | XIS1_460115Up inserted in pBluescript SK- | This study |
pBlueXIS1_460115UpDn | XIS1_460115Dn inserted in pBlueXIS1_460115Up | This study |
pBlueXIS1_460115UpKanDn | Kan cassette from pKanWOR inserted in pBlueXIS1_460115UpDn | This study |
pKR100 | oriR6K suicide vector, Cmr | H. Goodrich-Blair |
pKRXIS1_460109 | XIS1_460109UpKanDn inserted in pKR100 | This study |
pKRXIS1_460115 | XIS1_460115UpKanDn inserted in pKR100 | This study |