Table 2 Primers used to validate the architecture of the Porphyromonas gingivalis genome
Primer Name | Primer sequence (5' to 3') | Tm (°C) |
|---|---|---|
rrn1F | TCCCCACCGGCAAAAACATC | 68.0 |
rrn1R | GAGATGTCCGAAAGTCCATGTCAC | 66.3 |
rrn2F | AGATAGCCAGTTTCGTTACGTCCG | 67.3 |
rrn2R | TACAGCAACGGTTACTTCCGCG | 68.6 |
rrn3F | CTATGGATATTCTGCGGTGTACGG | 66.3 |
rrn3R | GTTGTAGGACAGCAACCTTTTGG | 64.2 |
rrn4F | ACAAGTCAGAACATGGCCGAT | 64.3 |
rrn4R | CAGGCACAAACCGCTTTACC | 65.0 |
ctnpg1_5out1 | GACGGAATTTGCGTGTTGATATAGT | 64.3 |
ctnpg1_5out2 | ATAAACGTGTGGCCGAAATAGATTC | 65.3 |
ctnpg1_5in | CAATAGCGTTTGCATTACCTCATCT | 65.3 |
ctnpg1_mid | ATCGGTGGAGATGTTCATACTACTG | 63.9 |
ctnpg1_3in | GTATTTGCCCAATACTCTCTGAACG | 64.9 |
ctnpg1_3out1 | CGACAACATCGTATTTCTCTGTCAG | 64.9 |
ctnpg1_3out2 | CACCGAGATTCAAGGTTATGTGATG | 66.9 |