Fig. 8

a and b Ceratocystis PI-PLC molecular model. a. (Left) Catalytic active region is shown in red interacted directly with the ligand inositol or phosphoinositol in Green. (Right) Detailed view of the active site of phospholipases C specific for phosphoinositol crystallographic (in cyan) and modeled (red). The detailed residues refer to the modeled protein. The mutations in Gln140 and Ser227 are conservative and capable of promoting interactions with the inositol molecule, necessary for the maintenance of phosphodiesterase activity. b Detailed view of the active site showed amino acid residues interact directly with the ligand. Amino acid residues of the template (Crystallographic resolve protein in PDB code 1AOD) are showed in cyan and PI-PLC Ceratocystis model in red. Mutations at Ser227 and Gln140 are conservative and able to promote interactions with inositol molecule, necessary for the maintenance of phosphodiesterase activity