Upregulation of genes encoding digestive enzymes and nutrient transporters in the digestive system of broiler chickens by dietary supplementation of fiber and inclusion of coarse particle size corn

Table 9 Effect of particle size and sugar cane bagasse on expression of jejunal genes at d 24^1,2

Treatments		APN	ASCT1	ATP1A1	B^oAT	CAT1	CAT2	CCK1R	CCK	EAAT3
Particle size	SB	APN	ASCT1	ATP1A1	B^oAT	CAT1	CAT2	CCK1R	CCK	EAAT3
CC	0%	1.071	1.386	1.206	1.058	1.000	1.025	1.004	1.136	1.188
FC	0%	1.103	1.000	1.083	1.062	1.099	1.113	1.066	1.163	1.124
CC	2%	1.148	1.222	1.196	1.037	3.236	1.000	1.048	1.000	1.083
FC	2%	1.000	1.197	1.000	1.137	1.451	1.126	1.021	1.134	1.000
Main effect
SB
0%		1.086	1.192	1.145	1.060	1.050^b	1.069	1.035	1.149	1.156
2%		1.074	1.155	1.098	1.087	2.343^a	1.063	1.034	1.066	1.041
Particle size
CC		1.109	1.304^a	1.202	1.048	2.119	1.013	1.026	1.067	1.136
FC		1.051	1.044^b	1.041	1.099	1.276	1.119	1.044	1.148	1.062
P value
SB		0.917	0.628	0.705	0.873	0.019	0.943	0.993	0.586	0.428
Particle size		0.644	0.003	0.199	0.762	0.111	0.232	0.886	0.596	0.611
SB × particle size		0.472	0.117	0.765	0.778	0.077	0.827	0.718	0.726	0.948

¹The relative expression levels of the genes of respective treatment groups are expressed as means of normalized relative quantities (NRQ). Relative quantities for individual genes are scaled to the average across all unknown samples per target gene
²Within a column, values with different superscripts are significantly different from each other at P < 0.05

ISSN: 1471-2164