Fig. 3

Expression profile of selected adipokines in visceral fat. a Expression profile in broiler and layer females was depicted from our RNA-seq data (Additional file 1: Table S2) calculated as fragments per kilobase of transcript per million mapped reads (FPKM) using edgeR and FDR value with threshold 0.05. Values are expressed as means ± SD. n = 3 in each group. *, FDR ≤ 0.05; **, FDR ≤ 0.01. APLN, IFNG, Leptin (LEP), SFTPA1, TNF, and ZP3, which are missing from the RNA-seq table either due to missing annotation in Galgal5 or low expression level, were searched manually in the RNA-seq data. P values of these transcripts were calculated by student t-test or rank transformed, in cases of lack of normal distribution, and expressed as means ± SE. b and c Expression profiles obtained by blast search using GenBank available experiments in two juvenile broiler experiments: fat and lean lines or high and low growth lines, respectively. The data was calculated as reads per kilobase of transcript per million mapped reads (RPKM). P values were calculated by student t-test and expressed as means ± SE. accession no. are detailed in the Material and Methods section