- Research article
- Open Access
Comparative omics and feeding manipulations in chicken indicate a shift of the endocrine role of visceral fat towards reproduction
BMC Genomicsvolume 19, Article number: 295 (2018)
The mammalian adipose tissue plays a central role in energy-balance control, whereas the avian visceral fat hardly expresses leptin, the key adipokine in mammals. Therefore, to assess the endocrine role of adipose tissue in birds, we compared the transcriptome and proteome between two metabolically different types of chickens, broilers and layers, bred towards efficient meat and egg production, respectively.
Broilers and layer hens, grown up to sexual maturation under free-feeding conditions, differed 4.0-fold in weight and 1.6-fold in ovarian-follicle counts, yet the relative accumulation of visceral fat was comparable. RNA-seq and mass-spectrometry (MS) analyses of visceral fat revealed differentially expressed genes between broilers and layers, 1106 at the mRNA level (FDR ≤ 0.05), and 203 at the protein level (P ≤ 0.05). In broilers, Ingenuity Pathway Analysis revealed activation of the PTEN-pathway, and in layers increased response to external signals. The expression pattern of genes encoding fat-secreted proteins in broilers and layers was characterized in the RNA-seq and MS data, as well as by qPCR on visceral fat under free feeding and 24 h-feed deprivation. This characterization was expanded using available RNA-seq data of tissues from red junglefowl, and of visceral fat from broilers of different types. These comparisons revealed expression of new adipokines and secreted proteins (LCAT, LECT2, SERPINE2, SFTP1, ZP1, ZP3, APOV1, VTG1 and VTG2) at the mRNA and/or protein levels, with dynamic gene expression patterns in the selected chicken lines (except for ZP1; FDR/P ≤ 0.05) and feed deprivation (NAMPT, SFTPA1 and ZP3) (P ≤ 0.05). In contrast, some of the most prominent adipokines in mammals, leptin, TNF, IFNG, and IL6 were expressed at a low level (FPKM/RPKM< 1) and did not show differential mRNA expression neither between broiler and layer lines nor between fed vs. feed-deprived chickens.
Our study revealed that RNA and protein expression in visceral fat changes with selective breeding, suggesting endocrine roles of visceral fat in the selected phenotypes. In comparison to gene expression in visceral fat of mammals, our findings points to a more direct cross talk of the chicken visceral fat with the reproductive system and lower involvement in the regulation of appetite, inflammation and insulin resistance.
The chicken was domesticated from its wild ancestor, the red junglefowl, about 6000 years ago. Since then, a variety of chicken breeds were created by geographical isolation and selection for desired traits . Diversifying selection of chickens for high growth rate and high egg production subsequently led to distinct phenotypes, characterized as broiler and layers . Thus, broiler and layer chickens provide a model system to study the genetic basis of these traits.
As a result of the breeding strategy, modern broilers reach about five times higher body weight (BW) during the fast-growing period before sexual maturation, compared to layers, and about four times higher body weight compared to the broiler strains of only six decades ago, which marked the beginning of intensive commercial breeding. Layer hens, on the other hand, produce almost one egg per day during the first year of lay, and in total more than 2.5 fold more eggs than broiler hens under feed restriction . Fertility and hatchability of the fertilized eggs are also higher in layers compared to broilers . Much of the difference in reproduction efficiency between the broiler and layer hens is associated with their ovarian morphology. Under ad libitum feeding, the ovary of a broiler breeding hen contains about twice as many yellow follicles than are present in a layer hen, resulting in disrupted hierarchy and hampered reproduction efficiency . In addition, broilers suffer from higher predisposition to pulmonary arterial hypertension, heart failure (ascites), impaired immunity, and higher insulin resistance compared to layers [6, 7]. Strong feed restriction regiments, used in the commercial growing of broiler breeders, greatly improve these metabolic disorders and correct the ovarian morphology . In layer hens, ad libitum feeding does not harm their production traits. However towards old age, layers can develop diseases such as fatty liver hemorrhagic syndrome, osteoporosis and hypocalcemia . We hypothesized that the adipose tissue may have a role in these traits, and that gene expression profiling on both the mRNA and protein levels may shed light on the regulatory mechanisms underlying these phenotypes in birds.
Adipose tissue stores excess triglycerides and releases them during periods of negative energy balance. In addition, since the discovery of the mammalian leptin in 1994 , it has become evident that the adipose tissue has a central endocrine role in mammals , operating by secretion of leptin and other signaling molecules, named adipokines . In mammals, adipokines are implicated in the regulation of many physiological processes, such as appetite and satiety, fat distribution, inflammation, blood pressure, hemostasis, insulin sensitivity, reproduction, immune response, and endothelial function [12, 13]. However, in birds much less is known about the expression of these adipokines and their endocrine role .
Leptin, the key adipokine in the endocrine activity of the fat tissue in mammals, has only recently been identified and characterized in birds [15,16,17,18] and its expression was detected in a variety of tissues, but with apparent insignificant expression in the adipose tissue [15, 16, 18]. We also recently identified an additional key adipokine, TNF (also called TNF-α), previously missing in chicken . Unlike leptin, TNF has a similar expression profile in chickens compared to mammals, including expression in the adipose tissue. These new findings emphasize the need for a combined analysis of global gene expression study and the analysis of candidate genes for unraveling the endocrine role of visceral adipose tissue in birds.
In the present study, female broiler breeder (Cobb) and layer (Leghorn, Lehmann) chickens were grown together under the same conditions with free access to food and water. At the day of hatch, the body weight (BW) of broiler and layer chicks differed by only about 10% (42.4 ± 0.4 and 38.3 ± 0.7 g, respectively). The difference in BW between the two strains, observed in the following weeks and at sexual maturation (21 weeks of age, broilers 6038 ± 113 g and layers 1503 ± 100 g; Fig. 1a), was huge as expected . Analysis of ovarian morphology revealed an excessive number of ovarian follicles (about 1.6-fold) in broilers compared to layers (Fig. 1b), which was also expected . Abdominal fat accumulation, measured at sexual maturation, showed no difference when calculated as the percent of BW (Fig. 1c). This observation, which is in contrast to previous publications , likely reflects the recent strong selection against excessive fat deposition in commercial broilers. Since this is the first demonstration that under a free feeding regiment, broiler and layer hens have similar relative body fat accumulation, we also compared abdominal fat accumulation at a weight of 1 kg (about 3 and 11 weeks of age for broilers and layers, respectively; Fig. 1a). Also at the same BW, no significant difference in visceral fat accumulation was observed (Fig. 1d).
In short, the broiler and layer hens used in the current study differed in the rate of body growth and ovarian morphology, reflecting the well-known higher body growth rate in broilers and higher egg production in layers . However, the modern broiler hens cannot be regarded as a model of obesity as suggested before .
Differential gene expression analysis in visceral fat by RNA-seq
To identify differentially expressed genes in visceral fat of broiler and layer chickens, we performed RNA-seq analysis on samples from three female broiler and three female layer chickens at the first week of lay (Additional file 1: Table S1). Sequence data were mapped to the reference genome assembly Galgal4 (GCA_000002315.2) and annotations were adapted to Galgal5 (GCA_000002315.3). Comparative analysis with P-values adjusted for false discovery rate (FDR) using edgeR revealed 1107 differentially expressed genes at FDR ≤ 0.05 and absolute fold change ≥1.5 (Fig. 2a; Additional file 1: Table S2). The set of differentially expressed gene was submitted to Ingenuity Pathway Analysis, using gene annotations of the corresponding human genes. A prominent elevation of the PTEN pathway was observed in broilers [Z-score 3.6; ratio 0.15; P < 0.001; Additional file 1: Table S3]. This pathway encompasses signals from growth factor receptors on the cell surface to transcription factors through inhibition of the PI3K/Akt signaling, which regulate cell proliferation, migration and survival , as well as glucose uptake and insulin sensitivity [23, 24]. As schematically demonstrated in Fig. 2b, the phosphatase and tensin homolog deleted on chromosome 10 (PTEN) mRNA was not differentially expressed between broilers and layers. However, transcripts implicated in the PTEN antagonistic signaling pathway PI3K/AKT and its downstream activities were lower in broilers (FDR ≤ 0.05; absolute fold change ≥1.5; MAST2, PIK3CA, MAPK1, FLT1, PIK3R1, FLT4, BMPR2, PREX2, CNKSR3, BCL2, SYNJ1, FOXO1, BMPR1A, PIK3CG, IGF1R, AKT3, KDR, ITGA4). Loss of PTEN leads to constitutive insulin sensitivity and obesity, in addition to high susceptibility to cancer . Therefore, the enrichment of increased expression of members of the PTEN pathway in broilers may explain their higher insulin resistance .
In layers, pathways that involve PI3K/AKT signaling were enriched as a mirror image of the enrichment of PTEN pathway in broilers. These include response to signaling by nitric oxide, IL8, relaxin, renin-arginine, leukocyte extravasation, HGF, VEGF, macropinocytosis, GH, NFAT, TEC kinase, eNOS, and NFkB (P < 0.001; Z-score ≤ − 2; Additional file 1: Table S3). Taken together, the pathway analysis suggests a biological difference of the metabolic activity in visceral fat in broilers and layers, stemming from a variation in the balance between two prominent signaling pathways: PTEN and PI3K/AKT. This difference correlates with the higher insulin resistance in broilers .
mRNA expression profile of adipokine and fat secreted proteins
Our main interest in adipose tissue relies on its known central role in the control of energy balance in mammals, mediated through secretion of adipokines with afferent metabolic signaling . To assess if there is a similar role of the adipose tissue in chickens, we focused on fat secreted proteins in the broiler and layer hens (Fig. 3a). The most prominent adipokines in mammals implicated in regulation of appetite, insulin sensitivity and inflammation including leptin, tumor necrosis α (TNF) interferon-γ (IFNG), and interleukin-6 (IL6) were expressed at low levels (below 1 fragments per kilobase of transcript per million mapped reads; FPKM) in both broilers and layers. Among the other characterized transcripts of known adipokines, the highest differential expression between broilers and layers was observed for the anti-inflammatory adipokine in mammals, adipolin (FAM132A, also known as C1QDC2 and CTRP12; ), which had about 14.7-fold higher level of mRNA in broilers (FDR = 6.5 × 10− 26). Two known hepatokines, lecithin cholesterol acyltransferase (LCAT; ) and cell-derived chemotaxin 2 (LECT2; ), were also expressed at a higher level in broilers (7.8-fold, FDR = 5 × 10− 8 and 8.9-fold, FDR = 4 × 10− 8, respectively). In layers, two known adipokines, retinol-binding protein 4 (RBP4) and adipsin (CFD), and two newly identified transcripts in the adipose tissue, zona pellucida protein 3 (ZP3) and pulmonary surfactant-associated protein A1 (SFTPA1) were expressed at a higher level (FDR/P ≤ 0.05; with fold differences of about 1.8, 2.1, 170 and 500, respectively).
Other transcripts coding for secreted proteins that we identified in visceral fat of broilers and layers were not differentially expressed. These included two known adipokines, adiponectin (ADIPO-Q) and apelin (APLN), nicotinamide phosphoribosyl transferase (NAMP1, also known as visfatin), and two transcripts characterized in the adipose tissue for the first time, glia-derived nexin (SERPINE2) and zona pellucida protein 1 (ZP1).
To assess the possible physiological implications of the expression profile described in Fig. 3a, we explored the expression of these genes also in RNA-seq data from visceral fat of two other chicken models that are available in GenBank. These include two broiler lines, divergently selected for fat and lean phenotype (FL-Br and LL-Br, respectively; ) and two other broiler lines divergently selected for high and low body growth (HG-Br and LG-Br; ). Both of these datasets represented mRNA expression in visceral fat of 7 weeks old males. At this age, FL-Br had on average a 2.6-fold higher accumulation of visceral fat than LL-Br and the same BW , while HG-Br had on average a 19.6-fold higher visceral fat accumulation than LG-Br and a 3.2-fold higher BW . Thus, also when normalized to BW, both FL-Br and HG-Br showed higher visceral fat accumulation compared to their respective controls (2.6- and 6.1-fold, respectively), with the HG-Br also differing in higher BW. Blast analyses of these datasets using the sequences of the selected fat secreted proteins as baits are shown in Figs. 3b & 3c. Similarly to the broiler-layer comparison, transcripts of the prominent adipokines in mammals, leptin, TNF, IFNG, and IL6 were low and did not show statistically significant differential expression. This suggests that leptin, TNF, IFNG, and IL6 are not implicated in the regulation of the pronounced phenotypic differences between these chicken lines. A general similarity in FPKM/reads-per-kilobase-of-transcript-per-million-mapped-reads (RPKM) levels of expression was observed between the sexually mature broilers and the juvenile broiler lines also for the prominently expressed genes ADIPOQ, APLN, NAMPT and SERPINE2, with a maximum of a 3-fold difference. Therefore, it is striking that the ZP1 mRNA was expressed at about an 800-fold lower level in the juvenile broilers compared to the sexually mature broiler hens. This observation is compatible with the expected role of ZP1 in female reproduction, and with the report by Hanafy et al., showing that expression of ZP1 in the liver of quail is induced by sexual maturation and by treatment with estrogen . It is likely that the ~ 10-fold lower mRNA levels of RBP4, LCAT and adipolin in the juvenile broiler lines compared to their expression in mature broiler females are also related to sexual maturation and reproduction. For LCAT, this has been indicated by Noble et al., which showed that plasma LCAT levels are induced by sexual maturation of layer hens and implicated in transporting HDL triglycerides to the egg yolk .
The search in the FL-Br and LL-Br data indicated that similarly to mammals, expression of adipolin and ADIPOQ correlates with lean phenotype. However, adipolin and ADIPOQ mRNA were expressed in HG and LG-Br at a similar mRNA level and was ~ 15-fold higher in broilers compared to layers, suggesting an additional role in promoting body growth. The proangiogenic factor ANGPT1  was expressed at a higher mRNA level in LL-Br compared to HF-Br, which is compatible with the known impairment of angiogenesis in obesity . Its non-differential expression in visceral fat of the HG-Br compared to LG-Br suggests that selection for normal expression of ANGPT1 was important to achieve high growth rate due to its critical role in angiogenesis . Comparing to LG-Br, a high level of transcription was detected in HG-Br for the atheroprotective peptide APLN (P = 6e− 9), which enhances glucose utilization and improves insulin sensitivity. CDF mRNA, which was expressed at a lower level in broilers compared to layers, was also expressed at lower levels in HG-Br compared to LG-Br, associating this gene with the restricted growth rate of chickens. LCAT, NAMP1, and SFTPA1 mRNAs were also found at a lower level in HG-Br (P ≤ 0.05). Among them, the SFTPA1 mRNA expression was strikingly 72-fold higher in LL-Br compared to FL-Br (P = 0.006), suggesting a role in restricting the rate of body growth and fat deposition. Altogether, the identification of new adipokines and secreted proteins by RNA-seq analysis of visceral fat of broilers and layers (LECT2, LCAT, SERPINE2, SFTPA1, ZP1, and ZP3), was confirmed also by the search in available RNA-seq data of juvenile broiler lines. Except for SERPINE2 and ZP1, the other mRNAs were differentially expressed between broiler and layer chickens and/or the experimentally selected lines of broiler. In contrast, some of the critical adipokines in mammals (leptin, TNF, IFNG and IL6) were lowly expressed and did not show differential expression between lines (P > 0.05).
Expression profiling of the newly identified fat secreted proteins LECT2, LCAT, SERPINE2, SFTPA1, ZP1, and ZP3 in tissues of red junglefowl
To more accurately estimate the tissue specificity of the secreted proteins that have not been previously observed in visceral fat of chicken, we used the available RNA-seq datasets of a variety of tissues from 2 years old red junglefowl [accession number ERA252218], which included 137 RNA-seq experiments from a male and a female bird (Fig. 4).
The expression of ZP1 and ZP3, which in mammals are synthetized solely by growing oocytes [35, 36], showed that ZP1 was dominantly expressed in both visceral fat and liver, while ZP3 mRNA was dominant in the ovary. The specificity of this expression to the female red junglefowl demonstrates the relevance of this expression to female reproduction. In other oviparous vertebrates, these proteins are found in the liver either in addition or instead of the ovary [37, 38]. In birds, extra gonadal expression of ZP1 was reported in the liver of quail , where it was also shown that intravenously injected ZP1 incorporates into the perivitelline membrane . Therefore, our finding of ZP1 and ZP3 expression in visceral fat suggests a chicken/bird specific mechanism of metabolic regulation of fertility.
The expression of the known hepatokines LCAT and LECT2 [48, 49] in the adipose tissue was also specific to the female red junglefowl, supporting their relevance to female reproduction. Implication of LCAT in female reproduction was suggested by Hengstschlager-Ottnad et al. , showing dynamic changes of LCAT expression in the liver and brain upon sexual maturation of laying chickens. Interestingly, although expected, LCAT was also transcribed in the liver , LECT2 mRNA was unexpectedly observed in the female lung. To verify that the sequence we used in this search is the genuine chicken LECT2, we compared local synteny of the human and chicken LECT2, and found the same neighboring genes (IL9 and TGFB1). Therefore, it seems that the chicken LECT2 gene has different function compared to its mammalian ortholog, including a role in the adipose tissue. As a control for the gender-specific expression, we searched the same datasets with the chicken RBM28 bait . RBM28 and other housekeeping genes that we have used in a similar search indicated that the gender specific pattern of ZP1, ZP3, LCAT, and LECT2 mRNA expression, is specific to these genes.
The pulmonary SFTPA1 and glial SERPINE2 genes in red junglefowl (Fig. 4) showed a relatively low level of expression of SFTPA1 in visceral fat and no significant expression for SERPINE2 (about 5 and below 0.1 RPKM, respectively). SFTPA1 was predominantly expressed in the lung and SERPINE2 in brain tissues, sciatic nerve, adrenal and proventriculus. These expression patterns fit with the known essential role of SFTPA1 in the structure and function of the pulmonary alveoli , and the pleotropic role of SERPINE2 in nerve and other cell types . Nevertheless, our finding of relatively high expression levels of SFTPA1 and SERPINE2 in visceral fat of commercial chickens (Fig. 3) suggests their recruitment to expression in visceral fat of chickens possibly due to selective breeding in the commercial lines. Although SFTPA1 and SERPINE2 were not implicated in adipose tissue before, their characterized roles as a phospholipid binding protein (SFTPA1; ), extra cellular matrix modification and inhibition of blood coagulation (SERPINE2; ) are compatible with a metabolic role in the adipose tissue. Altogether, the expression profile analysis in red junglefowl revealed recruitment of gene expression to the adipose tissue in chickens for ZP1, ZP3, LCAT and LECT2, either in addition (ZP3 and LCAT) or instead (ZP1 and LECT2) of their known expression pattern in mammals. The higher expression of Zp1,Zp3, LCAT and LECT2 in visceral fat of the female compared to the male, further supports their implication in female reproduction. For SFTPA1 and SEPRINE2 the study suggests a putative role in the phenotypic difference between wild and domestic chicken lines.
Effect of 24 h feed deprivation on adipokine expression
To further estimate the possible implication of the secreted proteins listed in Fig. 3 in the control of energy balance, we explored the effect of feed deprivation on their transcription. Broiler and layer females were grown as described in Fig. 1, but, immediately before tissue sampling, half of the birds of each type were subjected to a 24 h feed deprivation. Relative BW loss following this feed deprivation was much higher in layers compared to broilers when calculated as percent BW loss (Fig. 5a), reflecting their higher energy expenditure and lower feed efficiency . Under this fasting condition, there was no significant (P ≤ 0.05) effect on the relative weight of the visceral fat between the two groups (Fig. 5b), suggesting that the liver glycogen stores were the dominant source of energy requirement during the feed deprivation period.
Expression analysis using qPCR in visceral fat of the broiler and layer hens (Fig. 5c) showed that feed deprivation down-regulated expression of adipolin and ADIPOQ mRNAs in both broilers and layers (P ≤ 0.05). In broilers feed deprivation up-regulated SFTPA1 mRNA and in layers feed deprivation down-regulated the expression of ANGPT1, CFD and ZP3 mRNAs and upregulated NAMPT mRNA (P ≤ 0.05). Down-regulation of ADIPOQ mRNA by feed deprivation in chickens was shown also by Maddineni, et al., , but this response, as well as that of adipolin and NAMPT, are at an opposite direction in mammals [11, 47, 48]. It will be of high interest to understand the physiological meaning of adipolin, ADIPOQ, ANGPT1 and CFD downregulation by feed deprivation while they are expressed at a higher level in LL-Br compared to FL-Br (adipolin, ADIPOQ and ANGPT1) or in LG-Br compared to HG-Br (CFD). In contrast, the elevation of NAMPT mRNA by feed deprivation is compatible with its higher mRNA expression level in LG-Br compared to HG-Br and suggests a role in restricting body growth.
The expression analysis by qPCR of the selected fat secreted proteins in broilers and layers (Fig. 5c) confirmed the differential expression between broilers and layers of adipolin, CFD, LCAT, LECT2, RBP4, SFTPA1 and ZP3 observed in the RNA-seq experiment (Fig. 3a). Furthermore, the qPCR experiment that was conducted on a larger number of birds (n = 7 per treatment), revealed an additional differential gene product, APLN, which were higher in layers (Fig. 5c; P ≤ 0.05). Interestingly, APLN is a known adipokine implicated in coagulation, insulin resistance and diabetes  and its higher expression in layers is consistent with the pattern of another pro-inflammatory adipokine in mammal, RBP4 .
In summary, expression of adipolin, ADIPOQ, ANGPT1, CFD, NAMPT SFTPA1 and ZP3 mRNAs responded to feed deprivation. NAMPT, SFTPA1 and ZP3 mRNAs were reduced by feed deprivation in either broilers (SFTPA1) or layers (NAMPT and ZP3), compatible with NAMPT and SFTPA1 higher expression level in LG-Br compared to HG-Br, and with SFTPA1 higher expression in LL-Br compared to FL-Br (Fig. 3). Thus suggesting a role of these gene products in low growth and lean phenotype. It is of high interest that feed deprivation down-regulated the mRNA expression levels of adipolin, ADIPOQ, ANGPT1, which were expressed at higher levels in LL-Br compared to the FL Br, and CFD, which had higher mRNA level in LG-Br compared to HG-Br. This result suggests that adipolin, ADIPOQ, ANGT1, and CFD are not implicated in signaling the amount of energy stores.
Mass spectrometry (MS) analysis
MS analysis is quantitatively limited compared to RNA-seq, but provides a direct evidence of protein expression. Therefore, we investigated whether or not MS analysis of visceral fat would provide an additional perspective on the activity of the adipose tissue. Three individual broilers and three layers, grown as described in Fig. 1, were sampled at the first week of egg-lay. Using MS analysis on extracts of visceral fat, we identified 422 proteins, of which 203 showed differential expression between broilers and layers (P ≤ 0.05; Additional file 3: Table S5). Among the differential proteins, 27 were higher in broilers and 176 were higher in layers (Additional file 4: Fig. S1). Pathway analysis using Ingenuity software based on the significantly differential proteins (P ≤ 0.05) showed enrichment in broilers of the intrinsic prothrombin activation pathway (Z-score = 1, P < 0.0001; Additional file 3 Table S6; Additional file 4; Fig. S1). The enrichment stemmed from the higher abundance of FGA, FGB, and COL18A1 in broilers and higher COL3A1 in layers, representing only 10% of the proteins in this pathway. This result pointed to a higher coagulation activity in broilers, which is associated in mammals with atherosclerosis, inflammation and insulin resistance [51, 52], and in broiler chickens with “wooden breast disease” . Enrichment of the coagulation pathway in visceral fat of chickens has been reported by Resnyk et al., based on RNA-seq analysis in LG-Br compared to HG-Br  and in LL-Br compared to HF-Br . However, the lack of agreement between the MS and RNA-seq expression patterns for the relevant genes in this pathway (Additional file 3: Table S6B) indicated that for the expression of these genes, processes affecting translational and/or post-translational modifications have a critical impact.
To estimate the correlation between the differential patterns observed by MS and RNA-seq, we compared the respective values of log2 fold-change and found no correlation: r2 = 0.03, for all the gene products identified by MS and r2 = 0.06, for only the differential MS proteins (P ≤ 0.05). It is likely that in addition to variation between the two types of analysis originated from post-translational modifications, the lower depth of the MS analysis also contributed to the low correlation. Complete contradictions between the MS and RNA-seq analyses was observed only for two gene products, apolipoprotein B-100 (APOB), which mediates internalization of LDL particles into cells  and fibrinogen beta chain (FGB), which is an integral part fibrin matrix formation, tissue regeneration and coagulation . Both these genes were expressed at a lower mRNA level in broilers compared to layers (FDR ≤ 0.05), but found at a higher level in broilers by MS (P ≤ 0.05). Higher plasma APOB is implicated in obesity, insulin resistance, inflammation and polycystic ovary [51, 54] and may relate to the excess of ovarian follicles in broiler hens under free feeding. Therefore, the observed higher APOB and FGB abundance in broiler compared to layers may help to explain the metabolic challenges in broiler breeders , and may suggest new strategies for their improvement.
Among the adipokines described in Figs. 3 and 6, ADIPOQ, CFD and SERPINE2 were identified by the MS analysis, indicating their relatively high protein level. While ADIPOQ was expressed at a higher protein level in broilers, CFD and SERPINE2 were higher in layers (P ≤ 0.05). Among these adipokines only CFD showed a similar differential pattern also at the mRNA level (FDR = 0.05), indicating that gene expression of ADIPOQ and SERPINE2 is regulated also at the translational/post translational levels. The differential pattern of ADIPOQ at the protein level is consistent with the higher adipolin mRNA expression in broiler, since both these gene products are prominent anti-inflammatory adipokines in mammals. Interestingly, both of these gene products responded similarly to feed deprivation and were enriched in LL-Br compared to FL-Br, at the mRNA level.
Another observation by MS, which was not observed at the mRNA level, was the higher-level of perilipin 1 (PLIN1) in layers and PLIN-3 in broilers (P < 0.005). These two lipid-droplet proteins replace one another in coating the lipid droplet, but only PLIN1 has the capacity of protecting the droplet lipids from lipolysis . Therefore, this finding suggests a lower lipolysis activity in layers than in broilers under free feeding regiment. Elevated mRNA expression of PLIN1 is chickens has been reported by Resnyk et al., in FL-Br compared to LL-Br , suggesting that selection for a higher LPIN1 expression promoted the higher fat accumulation in the FL-Br. It is likely that for the commercial broiler line, the higher feed efficiency compared to layers relays on improved feed digestibility  rather than protection against lipolysis, suggesting a mean of further improvement of feed efficiency in broilers.
The lower sensitivity of the analysis by MS compared with mRNA data directed the focus on high abundant proteins in the visceral fat of broilers and layers. Among these proteins, we found egg-yolk proteins, which are expressed predominantly in the liver of oviparous species such as chickens (e.g. [58, 59]), but in non-avian oviparous species they were reported also in the adipose tissue . Out of the four major egg yolk proteins, vitellogenin-1 (VTG1), vitellogenin-2 (VTG2), albumin (ALB), and apovitellenin-1 (APOV1) (Fig. 6a; ), two were detected by MS at higher expression in layers (VTG2 and ALB, P ≤ 0.01; Fig. 6b). Our data from the RNA-seq analysis showed higher expression (FDR ≤ 0.05) in layers for VTG1, VTG2, ALB and APOV1 (Fig. 6c). Despite the higher number of ovarian follicles in broilers (Fig. 1), a lower production of the egg-yolk proteins was observed in their adipose tissue. These new finding of substantial expression of egg yolk proteins and mRNAs in visceral fat of chickens, together with additional mRNAs coding for the reproduction related proteins (LCAT, ZP1 and ZP3), suggest a stronger direct cross talk between the adipose tissue and the reproduction system in chickens than previously thought.
The comparison of gene expression in visceral fat of commercial layer and broiler chickens under normal conditions, as well as when exposed to feed withdrawal, shed new light on the function of adipose tissue and its evolution in chickens. Pathway analysis based on RNA-seq data showed higher response to signaling in the adipose tissue of layers and elevated expression of genes in the PTEN pathway in broilers. Similar analysis on the MS results indicated enrichment in broilers of intrinsic prothrombin activation pathway implicated in coagulation and inflammation. These characterizations at the mRNA and protein levels may explain the metabolic challenges of broiler breeders when grown under free feeding, which enforces the need for a strong feed restriction in the broiler breeder industry.
Comparisons of our data with available RNA-seq experiments on visceral fat with broiler lines selected for high and low visceral fat accumulation or for high and low body weight provided a broader perspective for the study as summarized in Fig. 7. This summary shows additional evidence to those described before  confirming that the chicken leptin is not involved in signaling from visceral fat. To our surprise, three additional prominent adipokines in mammals, TNF, IFNG and IL6 were also lowly expressed in visceral fat of the physiologically different chicken lines and following feed deprivation of broiler and layer females. Since these adipokines in mammals affect appetite, inflammation and insulin resistance, it is likely that the adipose tissue in chicken is not as critically involved in these endocrine regulatory routes as it is in mammals. Although we cannot exclude the possibility that different key genes and pathways than those operating in mammals, are effective in the endocrine regulation in chickens. In this regard, it is also important to note that three additional prominent adipokines in mammals: resistin (RETN), omentin (ITLN1) and plasminogen activator inhibitor-1 (PAI-1), which are implicated in the control of insulin resistance and inflammation are missing from the chicken genome assembly , and were not identified by our thorough search in RNA-seq data of adipose tissue . Our suggestion of a limited implication of chicken visceral fat in the regulation of energy balance in chicken compared to mammals is further supported by our observation that the excess of ovarian follicles in broilers is not related to obesity. In contrast, in mammals the polycystic ovary is associated with the obesity syndrome and an excess of inflammatory adipokines [62, 63].
The expression profile of known adipokines in mammals expressed in the chicken visceral fat, including adipolin, ADIPOQ, ANGPT1, APLN, and CFD, did not coherently reflect the amount of fat stores. Among them, the two most prominent anti-inflammatory adipokines in mammals, adipolin and ADIPOQ, were expressed at a higher level in broilers than in layers (on the mRNA or protein level, respectively), and also in LL-Br compared to FL-Br. However, adipolin and ADIPOQ responded to feed deprivation at an opposite direction than their mammalian orthologs [11, 47, 48], as they were down- instead of up-regulated. Similarly, the proangiogenic ANGPT1, and the proadipogenic CFD, were expressed at a higher mRNA level in the LL-Br compared to FL-Br or in the LG-Br compared to HG-Br, respectively, but were down-regulated by feed deprivation. APLN was expressed at a higher mRNA level in layers compared to broilers and in HG-Br compared to LG-Br. This expression pattern is inconsistent with a possible implication in regulation of body growth and appetite. Similarly, CFD, which was expressed at a higher levels in layers compared to broilers (both at the mRNA and protein level) and in LG-Br compared to HG-Br (at the mRNA level), was unexpectedly downregulated by feed deprivation. RBP4 was expressed at a higher mRNA level in layers compared to broilers, but was not dynamically expressed in the juvenile broiler lines, where it was lowly expressed (RPKM < 1; Fig. 4), suggesting its association with sexual maturation. Among the known adipokines in mammals, only expression of NAMPT seem constituent with energy stores as it was elevated by layer feed deprivation and in LG-Br. However, this correlation is limited since NAMPT was not differentially expressed in the LL vs. FL-Br, and did not respond to feed deprivation in broilers. Altogether, the expression profiles of the known adipokines in mammals that were dynamically expressed in visceral fat of chickens, seem not to report the amount of fat stores.
Some of the newly identified adipokines and fat secreted proteins (LCAT, LECT2, SERPINE2, ZP1, APOV1, VTG1 and VTG2), did not respond to feed deprivation in the broiler and layer females. The expression profile of LCAT, LECT2, SERPINE2, SFTPA1, ZP1, ZP3 mRNAs in red junglefowl tissues confirmed the recruitment of LCAT, LECT, and ZP1 to visceral fat of mature female chickens and suggested that SERPINE2, SFTPA1 and ZP3 were recruited to expression in visceral fat by the selective breeding of the commercial chicken lines. For SERPINE2 expression in visceral fat of layers was indicated also by MS and for SFTPA1, the high mRNA expression in layers (188 RPKM) was confirmed by its high expression also in LG-Br compared to HG-Br and in LL-Br compared to FL-Br. This dynamic expression profile of SFTPA1 mRNA was consistent with low growth rate and low fat accumulation. The possibility of a metabolic role of SFTPA1, in addition to its known critical function in the alveoli of the lung , was recently suggested by Rodgaard et al. , showing its upregulation at the mRNA level in the liver of obese pigs. Altogether, despite the known capacity of SFTPA1 to bind phospholipids in the pulmonary alveoli , understanding the possible role of SFTPA1 in visceral fat of the commercial chicken lines remains an interesting challenge.
The new finding of the reproduction related proteins ZP1, ZP3, APOV1, VTG1 and VTG2, in chicken visceral fat, suggests a more direct cross talk between the adipose tissue and the reproductive system in chickens compared to mammals. The mRNA expression pattern of adipolin, LCAT, LECT2 and RBP4 also suggest their implication in regulation of reproduction. This is based on their lower expression in the juvenile broiler lines compared to sexually mature broilers, and lower expression of LCAT and LECT2 in the visceral fat of male compared to the female red junglefowl.
Recent studies have shown that the correlation, between mRNA and protein expressions can be low due to translational/post-translational modifications . Nevertheless, in the current study, MS analysis indicated the recruitment of egg yolk protein expression to visceral fat of chickens and their higher expression level in layers compared to broilers, similarly to the indication found by RNA-seq. The MS analysis also indicated higher level of CFD protein in layers compared to broilers as found also at the mRNA level. An important contribution of the MS analysis was the indication of a higher ADIPOQ, SERPINE2, ABOB and FGB expression level in broilers, which were either non differential (ADIPOQ, SERPINE2) or higher in layers (ABOB and FGB) at the mRNA level. This confirmed the general conclusion that broilers express mixed markers of lean and fat phenotypes compared to layers and cannot be regarded as a model of obesity, even at ad libitum feeding. By comparing the relative accumulation of visceral fat in the two strains, we confirmed that the current broiler line does not accumulate higher relative amount of visceral fat compared to layers.
Our study shows specific evolutionary adaptations of gene expression in visceral fat of chickens towards a more direct interaction with the reproductive system. The central role of the mammalian adipose tissue in the control of appetite, inflammation and insulin sensitivity seems to evolve differently in mammals and chickens. It is possible that different gene products and molecular pathways mediate this regulation in visceral fat of chickens. However, it is also possible that along the evolution of chickens/avian species the endocrine control of reproduction by the adipose tissue was more critical for survival than the control on other aspects of energy balance.
Animals and tissue sampling
Female broiler (Cobb) and White Leghorn (Lohman) chickens were purchased from commercial husbandries (Brown & Sons and Hasolelim, Israel, respectively) at the age of 1 day and raised in the Volcani Center in Israel, according to recommended husbandry and feeding conditions (NRC 1994) with free access to food and water (n = 50 for each strain). Maintenance conditions and feeding formulas for both strains were according to the Lohman guideline (http://www.hylinena.com/userdocs/products/lohmann_brown_lite_commercials_2011.pdf). This contributed to the uniformity in the experiment and is justified by the fact that the commercial formula for broiler breeders (more condensed with proteins and fat) was optimized for feed restricted regiment and may not suit free feeding. Three similar experiments were performed: Two of the experiments ended when 10 out of the 50 reached sexual maturation (indicated by egg lay, during 4 month of age), samples of abdominal (visceral) fat, hypothalamus, and pituitary were snap-frozen in liquid nitrogen after neck dislocation. Samples collected in the first experiment were used for RNA-seq analysis and samples from the second experiment were used for qPCR and MS. The third experiment ended when 10 birds from each strain reached 1 kg BW (3 weeks for broilers, 11 weeks for layers) and samples were used for measuring the relative amount of visceral fat described in Fig. 1D. At the end of the experiments, the birds were killed by neck dislocation with no use of anesthetic or euthanasia methods. All animal procedures were carried out in accordance with the National Institutes of Health Guidelines on the Care and Use of Animals and Protocol IL536/14, which was approved by the Animal Experimentation Ethics Committee of the Agricultural Research Organization, Volcani Center, Bet-Dagan, Israel.
Total RNA was prepared using a RNA isolation kit (miRNeasy, Qiagen). Libraries of cDNA were prepared by the Uppsala sequencing platform from 1 μg RNA using the TruSeq Stranded mRNA Sample Preparation Kit (Illumina Inc., #15031047 Rev. E). The libraries were uniquely tagged and sequenced with Illumina HiSeq, producing about 64 million paired-end reads (2 × 124 bp) in two technical replicates, per library (S1 Table).
Bioinformatic analysis RNA-seq analysis
RNA-seq was performed as previously described . Briefly, Trim Galore! (http://www.bioinformatics.babraham.ac.uk/projects/trim_galore) and Trimmomatic  were used to remove adaptor sequences and low-quality base calls from the sequencing reads. Firstly, Trim Galore! 0.3.7 was used with the parameters “-q 15 -e 0.1 -O 6 --length 36”. The second replicate was further quality-trimmed using Trimmomatic 0.32 with the parameters “SLIDINGWINDOW:10:30 LEADING:5 TRAILING:5 MINLEN:36” to remove regions of low-quality base calls. Resulting single-end reads were removed before further analysis. The quality of the resulting reads was verified using FASTQC (http://www.bioinformatics.babraham.ac.uk/projects/fastqc). Alignment was done to galGal4 using GSNAP and gene expression was quantified using featureCounts and Ensembl release 79. The two technical replicates were averaged and differential expression analysis was done using the GLM method in edgeR and an FDR threshold of 0.05.
The search for gene expression in the short read archive (SRA) database was performed using 3 RNA-seq experiments: (i) The ChicksPress project of red junglefowl (http://geneatlas.arl.arizona.edu/sra_data.php; [accession number ERA252218]), which includes 137 RNA-seq experiments from a male and a female red junglefowl at the age of 2 years from variety of tissues. (ii) Data of visceral fat from 7 weeks old broiler chickens divergently selected for fatness or leanness at the same BW (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE42980; [Accession no. GSE42980] , obtained after 30 generation of selection with 3 biological and 2 technical replicates. (iii) Data of visceral fat from 7 weeks old broiler chickens divergently selected for high and low growth rate (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE49121; [accession no. GSE49121]; ). Accession numbers of sequences used as bait for blast search in RNA-seq experiments were: GenBank: APLN, XM_015278386; IFNG, GQ421600; leptin (LEP), LN794246 ; TNF, PRJEB13623 , SFTPA1, NM_001039166; ZP3, AB031033 ZP1, NM 204683; LCAT, NM_001293094; LECT2, NM_205478; SERPINE2, XM_015276900 and RBM28 .
Ingenuity Pathway Analysis (IPA; Qiagen, Valencia, CA; https://www.qiagenbioinformatics.com/products/ingenuity-pathway-analysis) software was used for canonical pathway analysis. Only genes showing differential expression with q < 0.05 (FDR corrected P values), total FPKM > 2, and absolute fold change > 1.5 were included.
Proteomics analysis was performed based on visceral fat from each of three individuals of each chicken strain. The sample preparation and mass spectrometry has been described previously  and the subsequent analysis was done similarly. In short, a protein fasta database was constructed using all annotated protein-coding genes and a spectrum matching method was employed to identify significant protein hits as described previously using a randomized version of the fasta as background .
Total RNA was prepared using a RNA isolation kit (miRNeasy, Qiagen) and cDNA was synthesized from 1 μg of total RNA using the Roche First Strand Synthesis Kit. QRT-PCR was performed using StepOnePlus Real-Time PCR System (Life Technologies and the Fast SYBR Green Master Mix (Life Technologies). All samples were analyzed in biological triplicates and technical duplicates. For several genes two different sets of primers were used (Additional file 2: Table S4), and gave similar results. Transcript levels were normalized to hydroxymethylbilane synthase (HMBS) and for ribosomal protein 17 (not shown), which gave similar results for all the tested mRNAs.
Statistical analyses of the RNA-seq was done using EdgeR and the false discovery rate (FDR) adjusted P-value using the Benjamini & Hochberg procedure, which is implemented in multtest package used for the analysis. qRT-PCR analyses were performed by one-way ANOVA and student’s t-test significant difference test (P ≤ 0.05). Induction of ZP1 mRNA expression upon sexual maturation (15.1 folds) was reported also by Bourin et al in the liver of layer type chickens (15.1 folds; ).
false discovery rate
fragments per kilobase of transcript per million mapped fragments
Gallus gallus reference genome
read per kilobase of transcript per million mapped reads
short read archive
Rubin CJ, Zody MC, Eriksson J, Meadows JR, Sherwood E, Webster MT, Jiang L, Ingman M, Sharpe T, Ka S, et al. Whole-genome resequencing reveals loci under selection during chicken domestication. Nature. 2010;464(7288):587–91.
Zuidhof MJ, Schneider BL, Carney VL, Korver DR, Robinson FE. Growth, efficiency, and yield of commercial broilers from 1957, 1978, and 2005. Poult Sci. 2014;93(12):2970–82.
Atzmon G, Cassuto D, Lavi U, Cahaner A, Zeitlin G, Hillel J. DNA markers and crossbreeding scheme as means to select sires for heterosis in egg production of chickens. Anim Genet. 2002;33(2):132–9.
Buzala M, Janicki B, Czarnecki R. Consequences of different growth rates in broiler breeder and layer hens on embryogenesis, metabolism and metabolic rate: a review. Poult Sci. 2015;94(4):728–33.
Hocking PM, Robertson GW. Ovarian follicular dynamics in selected and control (relaxed selection) male- and female-lines of broiler breeders fed ad libitum or on restricted allocations of food. Br Poult Sci. 2000;41(2):229–34.
Buzala M, Janicki B. Review: effects of different growth rates in broiler breeder and layer hens on some productive traits. Poult Sci. 2016;95(9):2151–9.
Shiraishi J, Yanagita K, Fukumori R, Sugino T, Fujita M, Kawakami S, McMurtry JP, Bungo T. Comparisons of insulin related parameters in commercial-type chicks: evidence for insulin resistance in broiler chicks. Physiol Behav. 2011;103(2):233–9.
Julian RJ. Production and growth related disorders and other metabolic diseases of poultry--a review. Vet J. 2005;169(3):350–69.
Zhang Y, Proenca R, Maffei M, Barone M, Leopold L, Friedman JM. Positional cloning of the mouse obese gene and its human homologue. Nature. 1994;372(6505):425–32.
Kershaw EE, Flier JS. Adipose tissue as an endocrine organ. J Clin Endocrinol Metab. 2004;89(6):2548–56.
Ahima RS, Lazar MA. Adipokines and the peripheral and neural control of energy balance. Mol Endocrinol. 2008;22(5):1023–31.
Freitas Lima LC, Braga VA, Do Socorro de Franca Silva M, Cruz JC, Sousa Santos SH, de Oliveira Monteiro MM, Balarini CM. Adipokines, diabetes and atherosclerosis: an inflammatory association. Front Physiol. 2015;6:304.
Friedman J: Leptin and the regulation of food intake and body weight. J Nutr Sci Vitaminol (Tokyo) 2015, 61 Suppl:S202.
Dakovic N, Terezol M, Pitel F, Maillard V, Elis S, Leroux S, Lagarrigue S, Gondret F, Klopp C, Baeza E, et al. The loss of adipokine genes in the chicken genome and implications for insulin metabolism. Mol Biol Evol. 2014;31(10):2637–46.
Friedman-Einat M, Seroussi E. Quack leptin. BMC Genomics. 2014;15:551.
Huang G, Li J, Wang H, Lan X, Wang Y. Discovery of a novel functional leptin protein (LEP) in zebra finches: evidence for the existence of an authentic avian leptin gene predominantly expressed in the brain and pituitary. Endocrinology. 2014;155(9):3385–96.
Prokop JW, Schmidt C, Gasper D, Duff RJ, Milsted A, Ohkubo T, Ball HC, Shawkey MD, Mays HL Jr, Cogburn LA, et al. Discovery of the elusive leptin in birds: identification of several 'missing links' in the evolution of leptin and its receptor. PLoS One. 2014;9(3):e92751.
Seroussi E, Cinnamon Y, Yosefi S, Genin O, Smith JG, Rafati N, Bornelov S, Andersson L, Friedman-Einat M. Identification of the long-sought leptin in chicken and duck: expression pattern of the highly GC-rich avian leptin fits an autocrine/paracrine rather than endocrine function. Endocrinology. 2016;157(2):737–51.
Bornelov S, Seroussi E, Yosefi S, Pendavis K, Burgess SC, Grabherr M, Friedman-Einat M, Andersson L. Correspondence on Lovell et al.: identification of chicken genes previously assumed to be evolutionarily lost. Genome Biol. 2017;18(1):112.
Griffin HD, Windsor D, Goddard C. Why are young broiler chickens fatter than layer-strain chicks? Comp Biochem Physiol A Comp Physiol. 1991;100(1):205–10.
Wang G, Kim WK, Cline MA, Gilbert ER. Factors affecting adipose tissue development in chickens: a review. Poult Sci. 2017;96(10):3687–99.
Lu XX, Cao LY, Chen X, Xiao J, Zou Y, Chen Q. PTEN inhibits cell proliferation, promotes cell apoptosis, and induces cell cycle arrest via downregulating the PI3K/AKT/hTERT pathway in lung adenocarcinoma A549 cells. Biomed Res Int. 2016;2016:2476842.
Hernandez R, Teruel T, Lorenzo M. Akt mediates insulin induction of glucose uptake and up-regulation of GLUT4 gene expression in brown adipocytes. FEBS Lett. 2001;494(3):225–31.
Wueest S, Rapold RA, Schoenle EJ, Konrad D. Fas activation in adipocytes impairs insulin-stimulated glucose uptake by reducing Akt. FEBS Lett. 2010;584(19):4187–92.
Stambolic V, Suzuki A, de la Pompa JL, Brothers GM, Mirtsos C, Sasaki T, Ruland J, Penninger JM, Siderovski DP, Mak TW. Negative regulation of PKB/Akt-dependent cell survival by the tumor suppressor PTEN. Cell. 1998;95(1):29–39.
Enomoto T, Ohashi K, Shibata R, Higuchi A, Maruyama S, Izumiya Y, Walsh K, Murohara T, Ouchi N. Adipolin/C1qdc2/CTRP12 protein functions as an adipokine that improves glucose metabolism. J Biol Chem. 2011;286(40):34552–8.
Ossoli A, Simonelli S, Vitali C, Franceschini G, Calabresi L. Role of LCAT in atherosclerosis. J Atheroscler Thromb. 2016;23(2):119–27.
Yoo HJ, Hwang SY, Choi JH, Lee HJ, Chung HS, Seo JA, Kim SG, Kim NH, Baik SH, Choi DS, et al. Association of leukocyte cell-derived chemotaxin 2 (LECT2) with NAFLD, metabolic syndrome, and atherosclerosis. PLoS One. 2017;12(4):e0174717.
Resnyk CW, Chen C, Huang H, Wu CH, Simon J, Le Bihan-Duval E, Duclos MJ, Cogburn LA. RNA-seq analysis of abdominal fat in genetically fat and lean chickens highlights a divergence in expression of genes controlling adiposity, hemostasis, and lipid metabolism. PLoS One. 2015;10(10):e0139549.
Resnyk CW, Carre W, Wang X, Porter TE, Simon J, Le Bihan-Duval E, Duclos MJ, Aggrey SE, Cogburn LA: Transcriptional analysis of abdominal fat in chickens divergently selected on bodyweight at two ages reveals novel mechanisms controlling adiposity: validating visceral adipose tissue as a dynamic endocrine and metabolic organ. BMC Genomics 2017, 18(1):626.
Hanafy AM, Sasanami T, Mori M. Sensitivity of expression of perivitelline membrane glycoprotein ZP1 mRNA in the liver of Japanese quail (Coturnix japonica) to estrogenic compounds. Comp Biochem Physiol C Toxicol Pharmacol. 2007;144(4):356–62.
Noble RC, Evans AJ, Crouchman ML. Lecithin--cholesterol--acyltransferase activity in the plasma of immature, oestrogenised and laying fowl (Gallus domesticus). Res Vet Sci. 1977;22(1):35–9.
Koh GY. Orchestral actions of angiopoietin-1 in vascular regeneration. Trends Mol Med. 2013;19(1):31–9.
Frisbee JC. Obesity, insulin resistance, and microvessel density. Microcirculation. 2007;14(4–5):289–98.
Epifano O, Liang LF, Familari M, Moos MC Jr, Dean J. Coordinate expression of the three zona pellucida genes during mouse oogenesis. Development. 1995;121(7):1947–56.
Izquierdo-Rico MJ, Gimeno L, Jimenez-Cervantes C, Ballesta J, Aviles M. Biosynthesis of hamster zona pellucida is restricted to the oocyte. Theriogenology. 2011;75(3):463–72.
Modig C, Modesto T, Canario A, Cerda J, von Hofsten J, Olsson PE. Molecular characterization and expression pattern of zona pellucida proteins in gilthead seabream (Sparus aurata). Biol Reprod. 2006;75(5):717–25.
Shi JW, Sheng JQ, Peng K, Wang JH, Yi WJ, Wu HJ, Gu Q, Hong YJ. Expression pattern of the zona pellucida 3 (ZP3) gene during ovarian development and the location of ZP3 protein in oocytes in a natural, wild triploid crucian carp mutant, Carassius auratus var. Pingxiangnensis. Genet Mol Res. 2013;12(4):5640–50.
Kinoshita M, Mizui K, Ishiguro T, Ohtsuki M, Kansaku N, Ogawa H, Tsukada A, Sato T, Sasanami T. Incorporation of ZP1 into perivitelline membrane after in vivo treatment with exogenous ZP1 in Japanese quail (Coturnix japonica). FEBS J. 2008;275(14):3580–9.
Hengstschlager-Ottnad E, Kuchler K, Schneider WJ. Chicken lecithin-cholesterol acyltransferase. Molecular characterization reveals unusual structure and expression pattern. J Biol Chem. 1995;270(44):26139–45.
Norum KR. The function of lecithin:cholesterol acyltransferase (LCAT). Scand J Clin Lab Invest. 2017:1–2.
Seroussi E, Pitel F, Leroux S, Morisson M, Bornelov S, Miyara S, Yosefi S, Cogburn LA, Burt DW, Andersson L, et al. Mapping of leptin and its syntenic genes to chicken chromosome 1p. BMC Genet. 2017;18(1):77.
Nathan N, Giraud V, Picard C, Nunes H, Dastot-Le Moal F, Copin B, Galeron L, De Ligniville A, Kuziner N, Reynaud-Gaubert M, et al. Germline SFTPA1 mutation in familial idiopathic interstitial pneumonia and lung cancer. Hum Mol Genet. 2016;25(8):1457–67.
Monard D. SERPINE2/protease Nexin-1 in vivo multiple functions: does the puzzle make sense. Semin Cell Dev Biol. 2017;62:160–9.
Swennen Q, Delezie E, Collin A, Decuypere E, Buyse J. Further investigations on the role of diet-induced thermogenesis in the regulation of feed intake in chickens: comparison of age-matched broiler versus layer cockerels. Poult Sci. 2007;86(5):895–903.
Maddineni S, Metzger S, Ocon O, Hendricks G 3rd, Ramachandran R. Adiponectin gene is expressed in multiple tissues in the chicken: food deprivation influences adiponectin messenger ribonucleic acid expression. Endocrinology. 2005;146(10):4250–6.
Dankel SN, Fadnes DJ, Stavrum AK, Stansberg C, Holdhus R, Hoang T, Veum VL, Christensen BJ, Vage V, Sagen JV, et al. Switch from stress response to homeobox transcription factors in adipose tissue after profound fat loss. PLoS One. 2010;5(6):e11033.
Smitka K, Maresova D. Adipose tissue as an endocrine organ: an update on pro-inflammatory and anti-inflammatory microenvironment. Prague Med Rep. 2015;116(2):87–111.
Boucher J, Masri B, Daviaud D, Gesta S, Guigne C, Mazzucotelli A, Castan-Laurell I, Tack I, Knibiehler B, Carpene C, et al. Apelin, a newly identified adipokine up-regulated by insulin and obesity. Endocrinology. 2005;146(4):1764–71.
Domingos MAM, Queiroz M, Lotufo PA, Bensenor IJ, Titan SMO. Serum RBP4 and CKD: association with insulin resistance and lipids. J Diabetes Complicat. 2017;31(7):1132–8.
Foley JH, Conway EM. Cross talk pathways between coagulation and inflammation. Circ Res. 2016;118(9):1392–408.
Lallukka S, Luukkonen PK, Zhou Y, Isokuortti E, Leivonen M, Juuti A, Hakkarainen A, Orho-Melander M, Lundbom N, Olkkonen VM, et al. Obesity/insulin resistance rather than liver fat increases coagulation factor activities and expression in humans. Thromb Haemost. 2017;117(2):286–94.
Mutryn MF, Brannick EM, Fu W, Lee WR, Abasht B. Characterization of a novel chicken muscle disorder through differential gene expression and pathway analysis using RNA-sequencing. BMC Genomics. 2015;16:399.
Vine DF, Wang Y, Jetha MM, Ball G, Proctor SD: Impaired ApoB-lipoprotein and triglyceride metabolism in obese adolescents with polycystic ovary syndrome. J Clin Endocrinol Metab 2016:jc20162854.
Casini A, Vilar R, Beauverd Y, Aslan D, Devreese K, Mondelaers V, Alberio L, Gubert C, de Moerloose P, Neerman-Arbez M. Protein modelling to understand FGB mutations leading to congenital hypofibrinogenaemia. Haemophilia. 2017;23(4):583–9.
Patel S, Yang W, Kozusko K, Saudek V, Savage DB. Perilipins 2 and 3 lack a carboxy-terminal domain present in perilipin 1 involved in sequestering ABHD5 and suppressing basal lipolysis. Proc Natl Acad Sci U S A. 2014;111(25):9163–8.
Sell-Kubiak E, Wimmers K, Reyer H, Szwaczkowski T. Genetic aspects of feed efficiency and reduction of environmental footprint in broilers: a review. J Appl Genet. 2017;
Ratna WN, Bhatt VD, Chaudhary K, Bin Ariff A, Bavadekar SA, Ratna HN. Estrogen-responsive genes encoding egg yolk proteins vitellogenin and apolipoprotein II in chicken are differentially regulated by selective estrogen receptor modulators. Theriogenology. 2016;85(3):376–83.
Wang H, Tan JT, Emelyanov A, Korzh V, Gong Z. Hepatic and extrahepatic expression of vitellogenin genes in the zebrafish, Danio rerio. Gene. 2005;356:91–100.
Tingaud-Sequeira A, Knoll-Gellida A, Andre M, Babin PJ. Vitellogenin expression in white adipose tissue in female teleost fish. Biol Reprod. 2012;86(2):38.
Abeyrathne ED, Lee HY, Ahn DU. Egg white proteins and their potential use in food processing or as nutraceutical and pharmaceutical agents--a review. Poult Sci. 2013;92(12):3292–9.
Chen CI, Hsu MI, Lin SH, Chang YC, Hsu CS, Tzeng CR. Adiponectin and leptin in overweight/obese and lean women with polycystic ovary syndrome. Gynecol Endocrinol. 2015;31(4):264–8.
Gursoy Calan O, Calan M, Yesil Senses P, Unal Kocabas G, Ozden E, Sari KR, Kocar M, Imamoglu C, Senses YM, Bozkaya G, et al. Increased adipsin is associated with carotid intima media thickness and metabolic disturbances in polycystic ovary syndrome. Clin Endocrinol. 2016;85(6):910–7.
Lopez-Rodriguez E, Pascual A, Arroyo R, Floros J, Perez-Gil J. Human pulmonary surfactant protein SP-A1 provides maximal efficiency of lung interfacial films. Biophys J. 2016;111(3):524–36.
Rodgaard T, Skovgaard K, Stagsted J, Heegaard PM. Cloning changes the response to obesity of innate immune factors in blood, liver, and adipose tissues in domestic pigs. Cell Reprogram. 2013;15(3):185–94.
Bolger AM, Lohse M, Usadel B. Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics. 2014;30(15):2114–20.
Bourin M, Gautron J, Berges M, Hennequet-Antier C, Cabau C, Nys Y, Rehault-Godbert S. Transcriptomic profiling of proteases and antiproteases in the liver of sexually mature hens in relation to vitellogenesis. BMC Genomics. 2012;13:457.
We thank Sergey Oblezin and Alexsander Kantor for chicken maintenance.
The study was supported by the Israel Academy of Sciences grants no. 876/14 and 1294/17, and Chief Scientist of the Israeli Ministry of Agriculture 0469/14 (to MFE and ES).
Availability of data and materials
The datasets generated during the current study are available in GenBank [Accession no. PRJEB23373].
All animal procedures were described before  and were carried out in accordance with the National Institutes of Health Guidelines on the Care and Use of Animals and Protocol IL536/14, which was approved by the Animal Experimentation Ethics Committee of the Agricultural Research Organization, Volcani Center, Israel.
Shane Burgess is a member of the editorial board (Section Editor) of BMC genomics. Other than that, all authors declare that they have no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
RNA-seq. Data. Table S1. Information about the RNA sequencing. Table S2: Transcripts identified by RNA-seq in visceral fat of broiler and layer females at the onset of sexual maturation. Table S3 Enriched pathways obtained using Ingenuity software and the RNA-seq differential transcripts (FDR ≤ 0.05; absolute fold change ≥1.5). A. List of enriched pathways. B. Schematic presentation of the enriched pathways. C. Expression pattern of the differentially expressed transcripts (FDR ≤ 0.05; absolute fold change ≥1.5) implicated in the in the PTEN pathway. Excel Worksheet xlsm 3.1 MB. (XLSX 3193 kb)
Table S4. The primers used for the qPCR analyses. Excel Worksheet xlsm 12 KB. (XLSX 11 kb)
MS data. Table S5. Proteins identified by MS in visceral fat of broiler and layer females at the onset of sexual maturation (422 proteins). Table S6. A. List of enriched pathways obtained using Ingenuity software and the MS differential proteins (P ≤ 0.05). B. List of proteins involved in the Intrinsic Prothrombin Activation Pathway: details on their expression at the protein and mRNA levels. Excel Worksheet xlsm 216 KB. (XLSX 142 kb)
Ingenuity pathway analysis of the MS data. Fig. S1A. Venn Diagrams showing the number of proteins with either no differential (ND) or differential expression between broilers (Br) and layers (La). n = 3 birds per strain, P ≤ 0.05, absolute fold change ≥1.5. B. Schematic presentation of the pathways highlighted by Ingenuity software (−log P value > 1.3). C. Schematic drawing of the intrinsic prothrombin activation pathway adapted from Ingenuity software. (DOCX 654 kb)