Fig. 4

Expression profile of miRNAs predicted to target the mouse Pax6 3’UTR. a Expression profile of miRNAs having predicted target sites in the mouse Pax6 3’UTR in various Pax6-expressing cells and tissues. miRNAs assayed for include those identified by our prediction analysis (Fig. 3a) as well as others, as described in the results. miRNAs were assayed for using TaqMan multiplex qPCR array cards. Data represents a total of 3 replicates per tissue or cell type, with a miRNA considered to be expressed only if the cycle threshold was less than 40 for all three replicates. b Relative miRNA level in mouse cultured pancreatic α cell line, αTC1–6 and a cultured β cell line, βTC6. c Relative miRNA level in mouse E12.5 retina, adult retina, and adult lens. Heat map indicates relative miRNA expression as a percentage of an internal control, snRNA U6. Data represents the geometric mean of three independent samples. Note the scale differences between the two heat maps