Host microRNA analysis in cyprinid Herpesvirus-3 (CyHV-3) infected common carp

BMC Genomics

Table 4 qRT-PCR validation of carp miRNAs expression during CyHV-3 infection

miRNA	Fold change (P2 v. P1) of miRNA expression.	Ct mean value \( \overline{X} \) ± SD,N = 18 measured by qRT-PCR, (P2 v. P1)	Student’s t-test and p value	Fold change (P3 v. P1) of miRNA expression.	Ct mean value \( \overline{X} \) ± SD,N = 18 measured by qRT-PCR, (P3 v. P1)	Student’s t-test and p value
mir-1	-1,86/DOWN	P2: 29,92 ± 3,93 P1: 27,96 ± 4,64	t = 2,69 p = 0,02*		P3: 29,72 ± 3,41 P1: 27,96 ± 4,64	t = 1,96 p = 0,07
mir-205		P2: 30,76 ± 2,84 P1: 32,57 ± 2,67	t = −1,71 p = 0,11	2,45/UP	P3: 30,07 ± 2,82 P1: 32,57 ± 2,67	t = −2,49 p = 0,03*
miR 144	2,04/UP	P2: 23,81 ± 1,54 P1: 25,11 ± 1,41	t = −3,42 p = 0,004*		P3: 24,19 ± 1,86 P1: 25,11 ± 1,41	t = −2,14 p = 0,05
miR-27a-5p		P2: 34,93 ± 0,99 P1: 35,23 ± 1,16	t = −1,25 p = 0,23	-1,39/DOWN	P3: 35,97 ± 1,13 P1: 35,23 ± 1,16	t = 2,48 p = 0,03*

Fold change – average change of miRNAs expression in 3 carps (s1, s2, s3) from each phase of infection), Ct value – comparison of average Ct value of qRT-PCR of 18 samples from experimental fish (including s1, s2, s3) between phases. Student’s t-test values for mean Ct values between phases(*) statistically significant differences between mean Ct values for p-value < 0.05

ISSN: 1471-2164