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Table 4 qRT-PCR validation of carp miRNAs expression during CyHV-3 infection

From: Host microRNA analysis in cyprinid Herpesvirus-3 (CyHV-3) infected common carp

miRNA Fold change (P2 v. P1) of miRNA expression. Ct mean value \( \overline{X} \) ± SD,N = 18 measured by qRT-PCR, (P2 v. P1) Student’s t-test and p value Fold change (P3 v. P1) of miRNA expression. Ct mean value \( \overline{X} \) ± SD,N = 18 measured by qRT-PCR, (P3 v. P1) Student’s t-test and p value
mir-1 -1,86/DOWN P2: 29,92 ± 3,93 P1: 27,96 ± 4,64 t = 2,69
p = 0,02*
  P3: 29,72 ± 3,41 P1: 27,96 ± 4,64 t = 1,96
p = 0,07
mir-205   P2: 30,76 ± 2,84 P1: 32,57 ± 2,67 t = −1,71
p = 0,11
2,45/UP P3: 30,07 ± 2,82
P1: 32,57 ± 2,67
t = −2,49
p = 0,03*
miR 144 2,04/UP P2: 23,81 ± 1,54 P1: 25,11 ± 1,41 t = −3,42
p = 0,004*
  P3: 24,19 ± 1,86
P1: 25,11 ± 1,41
t = −2,14
p = 0,05
miR-27a-5p   P2: 34,93 ± 0,99 P1: 35,23 ± 1,16 t = −1,25
p = 0,23
-1,39/DOWN P3: 35,97 ± 1,13 P1: 35,23 ± 1,16 t = 2,48
p = 0,03*
  1. Fold change – average change of miRNAs expression in 3 carps (s1, s2, s3) from each phase of infection), Ct value – comparison of average Ct value of qRT-PCR of 18 samples from experimental fish (including s1, s2, s3) between phases. Student’s t-test values for mean Ct values between phases(*) statistically significant differences between mean Ct values for p-value < 0.05