Fig. 6

Comparison of read coverage across H-locus region of chromosome 23 among Sri Lankan L. donovani CL and VL isolates. a L. donovani H-locus region is defined between the coordinates 80,157 bp – 102,529 bp on chromosome 23. Few isolates were chosen randomly among CL and VL groups to clearly represent the difference in amplification of the H locus region; CL isolates - L2339 (Green), L2343 (Blue), R5 (Black) and VL isolate - VL38 (Red). Amplification of the H-locus region in VL-SL compared to CL-SL isolates were clearly observed in genes; Terbinafine resistance locus protein (LdBPK_230280: 89042–89,611), MRPA (LdBPK_230290: 91285–95,325) and Argininosuccinate synthase gene (LdBPK_230300: 96936–98,192). Figure was generated using Artemis tool (b) Read coverage across chromosome 23 in six CL-SL and two VL-SL L. donovani isolates. Amplification of the H-locus (80,157 bp – 102,529 bp) is clearly seen among VL-SL VL38 isolate (In VL28 the H-locus amplification was not prominent probably due to comparatively low coverage of the sample) and one CL-SL isolate was an exception (L2331). Figure was generated using R graphics