Fig. 4
From: A high-density BAC physical map covering the entire MHC region of addax antelope genome
PCR verification of the overlap between pairs of overlapped BAC clones. Pairs of overlapped clones were PCR-amplified using the same primer pair designed based on the BAC-end sequence. The markers above the black lines indicate the primer pairs, and the ones below the lines are labels of positive clones used as PCR templates. M: DNA size standard (DL2000 Plus DNA Ladder from Transgene); the base pair (bp) sizes are indicated on both sides. a-d Validation of the overlap between clones covering the addax MHC class I–class III-class II a region. e Validation of the overlap between clones covering the addax MHC II b region