Table 1 Comparative primers used for the identification of positive BAC clones in the MHC regiona
From: A high-density BAC physical map covering the entire MHC region of addax antelope genome
Name | Gene symbol | Primer sequence (5′ → 3′) | Product (bp) | Positive addax BAC clones |
|---|---|---|---|---|
S0 | MOG | F: TGCATGCTGAGACTACACCA | 572 | 97H9;427 M6 |
R: CTGAACTAGGACAGTGCAGG | ||||
S02 | PPP1R11 | F: AGAACCGGAGCCTAACCATC | 641 | 97H9;427A11; 372A2 |
R: CCGCACACAGTGTGTATGAC | ||||
S03 | TRIM10 | F: ATGTGTGTGACCATGAGGCT | 650 | 427A11;470I19 |
R: TCAGTCTAGTCGCATCCGAG | ||||
S04 | TRIM26 | F: CCTTGCATGAAGTCAGCTTG | 919 | 470I19 |
R: CCACCTCTGAGCTGAATGAA | ||||
S05 | OLA-I | F: ACACCGCACTCGGCTACTAC | 332 | 273H17;419 N6; 493 K20 |
R: GCGATATAATCTCTGCCGTC | ||||
S06 | LOC101110710 | F: AGATCTCCAAGCTCAAGCCT | 805 | none |
R: TGTGTGTCTGACAGCTGCGA | ||||
S07 | ABCF1 | F: GCGAAGCCAGAGTTACCATA | 1119 | 427P1;392 L11; 51E6 |
R: CCACAGCATTCTCAAGCACA | ||||
S08 | ATAT1 | F: ATAGTGTGAAGGCCATGAGG | 908 | 427P1;427H1; 397 K7 |
R: GCTCAAGTTCTTGGATCGGT | ||||
S09 | NRM | F: GCTTGAGAGTGCCTTGGTAA | 514 | 350H17 |
R: ATGAGCTCTGCGTAGTCGAA | ||||
S10 | FLOT1 | F: GCACAGAAGTTGTCAGCACC | 911 | 350H17;297P6 |
R: TCGTCATTCTCTCTGGAAGC | ||||
S11 | DDR1 | F: GACTCCATGTTGTGCAGGCT | 1114 | 97P6;206A17; 132D20 |
R: GTCCTCACCTGGCATTCTTG | ||||
S12 | DPCR1 | F: CTAACATCACAGAGGCAAGC | 1129 | none |
R: TAAGTGAATGTGTGCGGAGC | ||||
S13 | CDSN | F: CAGTAACGCTGTGAGGAAGG | 830 | 395 M8;132D20; 205C9 |
R: GAGATGGCAGCTTATTCTGG | ||||
S14 | POU5F1 | F: CTCGGACCTGGATGAGCTTC | 1256 | 345 K6;395 M8 |
R: TATTCAAGGCTCAGCAGTCG | ||||
S15 | MICA | F: AACTCCTGGCAGAGATCTTG | 1064 | 345 K6;368L6 |
R: CTCACTGTCCACTTCTGGCT | ||||
S16 | LOC101105609 | F: CTCAACAGAGTTCGCGATCA | 851 | none |
R: AAGCTAGAGTCTGGCCTCCT | ||||
S17 | MCCD1 | F: GGCTCACCAGTTCAACCAAG | 747 | 217P17 |
R: CACTGCGTGTAGAGCTCGTC | ||||
S18 | TNF | F: GGAACCAGAGGATAAGCTGA | 980 | 217P17;269 L19 |
R: TCATCTGGAGGAAGCGGTAG | ||||
S19 | APOM | F: ACGACGCCAATCACAGTAAG | 986 | 145D13 |
R: CTCAACGAGGACGATGGTAA | ||||
S20 | LY6G6C | F: GGATGTGGTTCACCTGGTCA | 904 | 493O11 |
R: CGTGGAGGTTACACACTTGC | ||||
S21 | VARS | F: CTTGGACTGGAATCGAGCCT | 839 | none |
R: AGCCTTGGACCTTGTAAGCA | ||||
S22 | HSPA1A | F: CTACGTGGCCTTCACCGATA | 1214 | 493O11;383F23 |
R: CCTCGTACACCTGGATCAGC | ||||
S23 | C2 | F: TGTGATAATCGTCCAGCTCC | 1549 | 299D13 |
R: CCGATGGCATAGATGTCTGA | ||||
S24 | STK19 | F: GGATTGTCCAGCTAGGCTTC | 1182 | none |
R: CCTACACACACCTCACCACG | ||||
S25 | TNXB | F: CCAGATGTGCATACCACTCA | 942 | 218D15;120D3 |
R: GGTGTGCTCAGCATCTTCCT | ||||
S26 | PBX2 | F: GATAATGACCATCACCGACC | 1074 | 279C4;500O21 |
R: CCAGAGTTGAGGAATCGGAG | ||||
S27 | NOTCH4 | F: TCCTGGTACTCCATTCTTGC | 1006 | 279C4;500O21 |
R: GATTGACACGCGTCTGGTTC | ||||
S28 | LOC101111058 | F: ACCAGGTGACTGTCCAGAGG | 1117 | 53B21;301E21 |
R: GTGAAGTACAGGCCAGCTCC | ||||
S29 | LOC106991808 | F: GCTGCCCATCTGTCCACGA | 658 | 485B7;430H19 |
R: AATTCAGTTGAAAGGCACACT | ||||
S30 | C20H6orf10 | F: CACACGTACCTTCGGCTCT | 616 | 415H14;82H18; 55D19 |
R: ATTCTTCTGTCCACGCACT | ||||
S31 | LOC105603755 | F: TTCCCTTTCAGGTTCTCACCA | 441 | 390I7;292P1 |
R: TGCACAGTTATGATTGTTGGAC | ||||
S32 | LOC105603754 | F: AGCTTCTTTGTGAAAACGCAT | 499 | 424B10;146 M21 |
R: GCTATTCCCCGTGGATACCAAA | ||||
S33 | LOC101110546 | F: CTTTGGATACAGTTACGCTCCT | 944 | 392G17;478N22 |
R: ACCAATGAAACAGAATAGAGCC | ||||
S34 | LOC105603751 | F: GAAGGCGTCAGTCCATACCC | 707 | none |
R: ACAGAGCCTTATGGTTCCGAA | ||||
S35 | LOC101110277 | F: GCCTCATCCGACAGCACCG | 795 | 420O12;368E9 |
R: CCTTTCCTTGTGCTACGCTT | ||||
S36 | BTNL2 | F: CCTCTCGCTCTGCTATGGTT | 861 | 473F22;473H22; 233P9 |
R: CAGAAGGCATGCACCACTTA | ||||
S37 | LOC101120871 | F: GGTGAACACGGTGTGCAGAT | 907 | 244 M7;392G17 |
R: ACCATGAGTTGTGCAGCTGA | ||||
S38 | DQA | F: CAGGAGTATTGCTGAGCAGG | 1274 | 244 M7;247O24 |
R: TGACAAGGACAGTGGCAATG | ||||
S39 | OVAR-DRB3 | F: GACAGGAGCTAGATTGGACC | 924 | 1 M15;372C2 |
R: CCATGACAGCCTGCACATAC | ||||
S40 | LOC101120118 | F: AGGCAACGTCCAATGGTACT | 1321 | 385F12;381B3 |
R: CATGAATCCGCTGCATTCTC | ||||
S41 | LOC101109220 | F: ATGATGACATCTGGCACAGG | 970 | 385F12;401K6 |
R: CAACAGAGGTATGAGCCAGG | ||||
S42 | OVAR-DRB1 | F: CCTGTAATGGAAGTCCTCTG | 1272 | 432 J13;134D8 |
R: GCCAACTTGCTCTCTATGCT | ||||
S43 | ELOVL5 | F: CAGTGCATGGACCTACGAAC | 1562 | 134D8;276 J2 |
R: CAGGAGGCAGATCTACATGA | ||||
S44 | GCM1 | F: TTGGAAGAGCTAGGAAGCAC | 1151 | 276 J2;466O4 |
R: CTCGAGTCACCAGATCCTTG | ||||
S45 | VPS52 | F: CGCTTGAAGGATTGGTGACT | 930 | 444E20 |
R: GACCATGATCTGCACTCAGG | ||||
S46 | RXRB | F: TGTACAGGAGGAGCGTCAGC | 973 | 354 J10 |
R: GGTGAGGTCAACGAGACTGG | ||||
S47 | LOC101110545 | F: TCTTCTCATCTCGCACACAG | 982 | none |
R: GAAGAAGCAGGCCTTGAATC | ||||
S48 | BRD2 | F: CTGTATACTCCACCGCCTGA | 900 | 270A14 |
R: GTGTGTGTGGCCTCCAACTA | ||||
S49 | PSMB9 | F: ACCAGATAACCTTGAGCCAG | 869 | 176F14 |
R: AGGATCATACAGCGAGTTGC | ||||
S50 | DYA | F: CGAGCTCTTCTACGTGGACC | 1037 | 94B2 |
R: GACCACAGCTTCCTCGTTCT | ||||
S51 | GCLC | F: TGATGGAACATCAGGCCTAC | 841 | 68 K22;427 K6 |
R: GAGAAGGCGTCCGACTATAA |