Fig. 6

Phylogenetic tree of the chemosensory protein (CSP) family. The protein sequences included were from Dendroctonus ponderosae (Dpon, red), Agrilus planipennis (Apla, blue), Anoplophora glabripennis (Agla, green), and Tribolium castaneum (Tcas, orange). The tree is based on a MAFFT alignment, constructed using FastTree, and unrooted. Numbers at nodes are local support values, calculated using the Shimodaira-Hasegawa (SH) test implemented in FastTree. Exact SH values are only shown if > 0.7, whereas SH values for all branches are indicated by the colored circles; support increases with the size and brightness of the circles. Black arcs indicate highly supported clades with a single CSP representative from each of the four species; grey arcs indicate highly supported clades with CSPs from all four species, with one of the species showing evidence of gene duplication. The sources of sequence data and explanation of protein suffixes are detailed in the Methods section