Genomic content of chemosensory genes correlates with host range in wood-boring beetles (Dendroctonus ponderosae, Agrilus planipennis, and Anoplophora glabripennis)
BMC Genomics volume 20, Article number: 690 (2019)
Olfaction and gustation underlie behaviors that are crucial for insect fitness, such as host and mate selection. The detection of semiochemicals is mediated via proteins from large and rapidly evolving chemosensory gene families; however, the links between a species’ ecology and the diversification of these genes remain poorly understood. Hence, we annotated the chemosensory genes from genomes of select wood-boring coleopterans, and compared the gene repertoires from stenophagous species with those from polyphagous species.
We annotated 86 odorant receptors (ORs), 60 gustatory receptors (GRs), 57 ionotropic receptors (IRs), 4 sensory neuron membrane proteins (SNMPs), 36 odorant binding proteins (OBPs), and 11 chemosensory proteins (CSPs) in the mountain pine beetle (Dendroctonus ponderosae), and 47 ORs, 30 GRs, 31 IRs, 4 SNMPs, 12 OBPs, and 14 CSPs in the emerald ash borer (Agrilus planipennis). Four SNMPs and 17 CSPs were annotated in the polyphagous wood-borer Anoplophora glabripennis. The gene repertoires in the stenophagous D. ponderosae and A. planipennis are reduced compared with those in the polyphagous A. glabripennis and T. castaneum, which is largely manifested through small gene lineage expansions and entire lineage losses. Alternative splicing of GR genes was limited in D. ponderosae and apparently absent in A. planipennis, which also seems to have lost one carbon dioxide receptor (GR1). A. planipennis has two SNMPs, which are related to SNMP3 in T. castaneum. D. ponderosae has two alternatively spliced OBP genes, a novel OBP “tetramer”, and as many as eleven IR75 members. Simple orthology was generally rare in beetles; however, we found one clade with orthologues of putative bitter-taste GRs (named the “GR215 clade”), and conservation of IR60a from Drosophila melanogaster.
Our genome annotations represent important quantitative and qualitative improvements of the original datasets derived from transcriptomes of D. ponderosae and A. planipennis, facilitating evolutionary analysis of chemosensory genes in the Coleoptera where only a few genomes were previously annotated. Our analysis suggests a correlation between chemosensory gene content and host specificity in beetles. Future studies should include additional species to consolidate this correlation, and functionally characterize identified proteins as an important step towards improved control of these pests.
Interpreting chemical information in the environment is of paramount importance for the fitness of animals. In insects, the chemical senses –olfaction and taste– underlie the ability to find mates, food, and oviposition sites, and to avoid harmful situations and non-host habitats . The chemosensory multi-gene families are typically the largest gene families of insect genomes, emphasizing the key role of olfaction and taste in insect ecology. In addition, understanding the ‘birth-and-death’ evolution of these genes is needed to gain insight into the mechanisms underlying ecological specialization, evolutionary divergence, and speciation [2, 3], and it may also reveal molecular targets that can be manipulated for insect control, such as pheromone receptors . How the ecology and life history traits of different species relate to the evolution of their chemosensory genes is, however, still an open question. To shed further light on this relationship, we targeted four different beetles (Coleoptera) that are keystone species in forest ecosystems and also serious pests, including the mountain pine beetle Dendroctonus ponderosae Hopkins (Curculionidae), the emerald ash borer Agrilus planipennis Fairmaire (Buprestidae), and the Asian longhorn beetle Anoplophora glabripennis Motschulsky (Cerambycidae). The aim was to investigate how their host breadth (stenophagy vs. polyphagy) may correlate with the diversification of genomic repertoires of chemosensory genes, and at the same time reveal genes that can be targeted for improved control of these forest pests .
Most insect chemosensory genes encode membrane-bound receptors from three divergent gene families, which generally are expressed in primary sensory neurons. The odorant receptors (ORs) represent the principal means of sensing volatile chemicals from a distance, including pheromones and odorants from plants and microbes . The receptor genes are mainly expressed in the olfactory sensory neurons (OSNs) of the insect antennae and maxillary palps. ORs are seven-transmembrane domain proteins with an intracellular N-terminus, and are unrelated to vertebrate ORs, which are G-protein coupled receptors [6,7,8,9,10]. In most cases, a single ligand-specific OR gene is expressed in each OSN together with the olfactory receptor co-receptor Orco, which is present in all insect genomes except for those of the most basal lineages [11, 12]. The OR and Orco proteins form a heterotetrameric receptor complex , where Orco is required for the formation of a ligand-gated cation channel, and hence odor-evoked responses in OSNs [14,15,16]. The more ancient and even more diverse family of gustatory receptors (GRs) [17, 18] belongs to the same superfamily as the ORs [19, 20]. These genes are expressed in a variety of tissues, and are mainly involved in contact chemoreception of e.g., sugar and bitter compounds, but also in the sensing of carbon dioxide [21, 22]. The third family encodes the ionotropic receptors (IRs), which are related to the conserved ionotropic glutamate receptors (iGluRs), but they have atypical binding domains and are expressed in a combinatorial fashion in sensory neurons [23, 24]. The conserved ‘antennal’ IRs are involved in olfaction [23, 25, 26], but also in the sensing of humidity, salt, and temperature [27,28,29]. Members of the ‘divergent’ group of IRs, which is highly variable within and between species, have been assigned a role in taste [25, 30].
Proteins from additional gene families play various other roles in insect chemosensation. Genes encoding the sensory neuron membrane proteins (SNMPs), which are related to scavenger proteins of the CD36 family, are expressed in certain OSNs that express OR genes . Two representatives (Snmp1 and Snmp2 genes) are found in most insect genomes , and a third representative (Snmp3) has been identified in Coleoptera . SNMP1 is important for pheromone responses in Drosophila melanogaster and moths [31, 34,35,36], whereas potential roles of SNMP2 and 3 in chemosensation remain unknown. The lymph of chemosensory sensilla also contains abundant small soluble odorant binding proteins (OBPs) [37, 38], which bind, solubilize, and transport hydrophobic odor molecules, and may also serve as a form of gain control, buffering changes in the odor environment [39,40,41,42]. Finally, members of the chemosensory protein (CSP) family, of which some are abundant in the sensillum lymph, have also been shown to bind odorants [43, 44], and may thus have similar roles as the OBPs. However, CSP genes are expressed also in non-chemosensory tissues and some have non-sensory functions [37, 45, 46].
The links between chemosensory gene evolution and ecological specialization are poorly understood, although it has been hypothesized that traits, such as host breadth and social behavior, may correlate with the diversification of these gene families . Analyses of insect genomes are crucial in addressing this question, because studies of antennal transcriptomes, which is a more common approach , generally miss a large proportion of the genes present in the genome (cf. [48, 49]). While chemosensory genes from the Diptera, Hymenoptera, and Lepidoptera have been more thoroughly compared in this regard [50,51,52], the largest order of insects, Coleoptera, remains poorly investigated. Apart from the OR genes, which were recently analyzed across ten coleopteran genomes , full genomic repertoires of GR, IR and OBP genes have only been identified in three species: the flour beetle Tribolium castaneum [54, 55], the Asian longhorn beetle A. glabripennis , and the Colorado potato beetle Leptinotarsa decemlineata . The CSP and SNMP genes have so far only been reported from the genome of T. castaneum [33, 57].
To test the hypothesis that variation in host range may be reflected in chemosensory gene repertoires, we annotated these genes in the genomes of two stenophagous wood-boring and phloem-feeding pests of the Coleoptera: the mountain pine beetle D. ponderosae, and the emerald ash borer A. planipennis. Proteins encoded by these genes were phylogenetically analyzed with those from the polyphagous species T. castaneum and A. glabripennis, with the latter species being particularly relevant because it also exhibits a wood-boring lifestyle, and it has been recorded from > 100 host plants . In contrast, T. castaneum feeds on a large variety of seeds and other dried foods. D. ponderosae is a devastating North American bark beetle, able to kill a number of pine (Pinus) species over landscape-scales through mass attacks coordinated by an aggregation pheromone [59, 60]. As a result of warming temperatures, current outbreaks have caused unprecedented economic loss, and turned North American forests into sources of carbon release . A. planipennis is native to Eastern Asia, but has become an invasive pest in North America, threatening the existence of several ash (Fraxinus) species . Compared to D. ponderosae, the chemical ecology of this species is less understood, although odor-mediated attraction to hosts and conspecifics has been shown [63,64,65]. Previous studies of the antennal transcriptomes of these wood-borers identified initial sets of chemosensory genes in D. ponderosae  and A. planipennis . However, subsequent evolutionary and functional analysis have been hampered because both datasets are incomplete (especially for A. planipennis), i.e., they include a comparatively small number of identified chemosensory genes, lack expected orthologues, and include many partial gene models. A recent study included the genomic annotations of the ORs of these two species in a large-scale analysis , but we here reanalyze them alongside the other chemosensory genes in the context of host specificity. Our analysis suggests a correlation between the genomic content of chemosensory genes and host range in beetles. The annotations presented here are an important contribution to the pool of known chemosensory genes within the Coleoptera, facilitating future evolutionary and functional studies, which in turn may lead to more efficient and sustainable pest control tactics.
Improved sets of chemosensory genes in D. ponderosae and A. planipennis
A previous study of the antennal transcriptome of D. ponderosae (“Dpon”) identified a total of 111 chemosensory genes, including 49 ORs, 2 GRs, 15 IRs, 3 SNMPs, 31 OBPs, and 11 CSPs . Our present study of the genome yielded a total of 254 chemosensory genes (Additional file 1: Table S1), of which 153 gene models were not identified in the previous transcriptome. New genes were identified in all gene families, with the largest increase observed for the GRs, followed by the ORs and IRs (details in sections below). Several of the original partial transcript sequences were extended (often to full length), and errors due to previously unnoticed frameshifts or introns on several original transcript models were corrected (Additional file 2: Table S2). Ten of the original gene models were discarded: one OR gene (previous DponOr45) was the result of a transcript chimera; one IR gene (previous DponIr56e.1) showed no homology to insect IRs; two IR gene fragments (previous DponIr21a.2 and 56e.2) were dropped because they were revealed to belong to the same genes as two other previously reported partial IR genes; one IR gene (previous DponIr93a.2), four OBP genes (previous DponObp17, 20, 24, 32), and one CSP gene (previous DponCsp5) were assembly isoforms or alleles of other genes (Additional file 2: Table S2). Only one gene model (DponOr21) that was complete in the transcriptome study was incomplete in the genome assemblies; hence the original model was retained.
In A. planipennis (“Apla”), 24 chemosensory genes (2 ORs, 2 GRs, 6 IRs, 1 SNMP, 9 OBPs, and 4 CSPs) were previously identified from an antennal transcriptome . Here, we annotated a total of 137 chemosensory genes from its genome, of which 118 annotations are novel compared to previous transcriptome work. Several of the original models were revised (Additional file 2: Table S2) or discarded: two OBP genes (previous AplaObp4 and AplaObp8) were discarded because they were assembly isoforms; two IR genes were identified as separate fragments of AplaIr25a (isotig01857-ApIR and G3QO8C008JMTAX_ ApIr); and both existing short AplaGR gene models were found to lack homology to insect GRs. Several of the original gene models were renamed, especially in D. ponderosae (most ORs, some IRs, and two SNMPs; see also ) to follow established nomenclature for genomic annotations of these gene families (see Methods section for details and Additional file 2: Table S2 for correspondence with original names).
A total of 86 OR genes, including Orco and 7 putative pseudogenes, were annotated in the genome of D. ponderosae, of which 63 OR genes were completed to full-length. Except for DponOR53INT (195 amino acids), all partial but putatively functional DponORs are above 350 amino acids in length, with the majority only missing a short N-terminal exon (named A1) that could not be confidently identified due to absence of transcriptomic support. As has been observed with the OR genes in other insect genomes, a large proportion of the genes in both species occur in tandem arrays on scaffolds (Additional file 1: Table S1). Although alternative splicing is uncommon in ORs, two of the DponOR genes were regarded to each encode two alternative splice variants (named DponOr2a/b and DponOr36a/b) with mutually exclusive N-terminal A1-A2 exons assembled consecutively with seemingly shared C-terminal B-E exons. The A. planipennis genome contains 47 OR genes, including Orco and one pseudogene. In this species, 31 of the ORs were completed to full-length models, with partial OR genes encoding protein sequences between 174 and 393 amino acids. In both species, the number of introns in full-length OR genes varies between four and seven, whereas Orco is interrupted by ten introns in both species (Additional file 1: Table S1; see also ). Both D. ponderosae and A. planipennis have fewer putatively functional OR genes and pseudogenes compared to other species considered polyphagous (Table 1).
The DponORs and AplaORs were recently included in phylogenetic analyses that covered ORs from ten coleopteran genomes, and which allowed for classification and revision of nine higher-order monophyletic OR subfamilies (designated as Groups 1, 2A, 2B, 3, 4, 5A, 5B, 6, and 7) across the Coleoptera . Several of these groups had been recognized also in earlier studies [54, 66]. In this study, the phylogenetic analysis was restricted to two additional species (T. castaneum “Tcas” and A. glabripennis “Agla”), and we can here afford to present and discuss the results for these particular species in more detail. Our phylogeny (Fig. 1) shows that the distribution of ORs among the nine major coleopteran OR subfamilies is species-dependent. The majority of DponORs belong to Group 7, followed by Groups 5A, 1, 2A, and 2B. In contrast, most AplaORs are found within Group 2B, followed by Groups 3, 6, 5B, and 2A. Furthermore, D. ponderosae appears to have lost ORs in Groups 3, 4, 5B, and 6, whereas A. planipennis lacks ORs in Groups 1, 4, 5A, and 7. These different OR distribution patterns are also distinct from those in T. castaneum and A. glabripennis, which in turn also are different from each other. In D. ponderosae, the largest species-specific radiation contained 30 ORs (DponOR27–55 including the putatively alternatively spliced DponOR36a/b in Group 7), whereas the largest expansion in A. planipennis contained 18 ORs (AplaOR16–33 in Group 2B). Well-supported orthologous relationships were only found for AglaOR55/DponOR57–59, AglaOR38/DponOR10–11, and TcasOR73FIX/DponOR56.
In D. ponderosae, we annotated 60 GR transcripts (including 57 full-length models and one pseudogene) that are encoded by 49 genes. Seven of these genes were regarded to exhibit alternative splicing, each producing either two or four splice variants (Additional file 1: Table S1). Most splice variants are encoded by genes with two exons, and they share the C-terminal exon, but have a unique N-terminal exon. One of the alternatively spliced genes, DponGr38a-d, has three exons, of which the N-terminal exon is unique and the two C-terminal exons are shared. In A. planipennis, 30 GR genes were revealed (22 full-length models), with no evidence of alternative splicing. The putative receptors for carbon dioxide and sugars contain several introns, whereas the majority of the remaining putative bitter-taste GR genes contain only one or two introns in both species. However, several of these GRs, especially in D. ponderosae, contained one to four additional introns (Additional file 1: Table S1). As with ORs, D. ponderosae and A. planipennis presented fewer putatively functional GR genes and pseudogenes compared to other species considered polyphagous (Table 1).
The Dpon and AplaGRs were phylogenetically analyzed together with the GRs from A. glabripennis and T. castaneum, showing that the three conserved GRs for carbon dioxide (GR1–3) are present in D. ponderosae, whereas no evidence of GR1 was recovered from the genome assembly of A. planipennis, nor from the available raw sequence reads (accession: SRR1174015–SRR1174018; Fig. 2). In addition, both species have six GRs (GR4–9) that grouped within the clade of conserved sugar receptors. Whereas DponGR4, DponGR6, and DponGR9 appear orthologuous to AglaGR4, AglaGR8, and AglaGR6, respectively, no simple orthologuous relationships are evident for the other sugar receptors in D. ponderosae or for any of these GRs in A. planipennis. These two species also have one GR each (GR10) that was placed within the clade of conserved fructose receptors, which is dominated by a lineage expansion in T. castaneum. Most of the remaining GRs (putative bitter-taste GRs) of all four species in the analysis grouped in small to large species-specific expansions, which in many cases comprise large suites of alternatively spliced proteins, especially from T. castaneum and A. glabripennis. Among the putative bitter-taste GRs, only a single clade was represented by one orthologue from each of the four species. This clade was highly supported (Shimodaira-Hasegawa [SH] support value 1.0) and named the “GR215 clade” based on the GR representative from T. castaneum (Fig. 2). The GR215 clade is part of a larger and well-supported subfamily that includes one additional DponGR (DponGR46), and large expansions of alternatively spliced proteins from T. castaneum and A. glabripennis. Finally, it is noteworthy that one of the largest well-supported GR lineages (indicated by the long black arc in Fig. 2), comprising almost half of the GRs in our analysis, was devoid of GR representatives from A. planipennis.
In D. ponderosae, we identified a total of 57 IR genes (51 full-length models), including two pseudogenes. The number of IRs in A. planipennis was 31 (22 full-length models), including one pseudogene. Members of the conserved antennal IR8a, IR21a, IR25a, IR40a, IR41a, IR68a, IR76b, and IR93a were identified in both species (Fig. 3). Two paralogues of IR41a and IR76b were annotated in D. ponderosae and A. planipennis, respectively. Furthermore, D. ponderosae has 11 IRs that fell within the IR75 clade, which to date is the largest number reported from a beetle genome. In contrast, A. planipennis has only four members in this clade. Both species also had members of the IR100a clade, with three receptors found in D. ponderosae, and one in A. planipennis. Each beetle species (including L. decemlineata “Ldec”) also has one IR that grouped with IR60a from D. melanogaster (“Dmel”) with high support (0.93), suggesting that this IR is conserved in beetles. Hence, the IRs from this group identified in the present study were named DponIR60a and AplaIR60a, whereas the orthologues from T. castaneum, L. decemlineata, and A. glabripennis retained their original names (TcasIR108, LdecIR106, and AglaIR150). The remaining divergent IRs from D. ponderosae and A. planipennis generally grouped in species-specific lineage expansions of various sizes, with only a few IRs being individually placed. Whereas the antennal IR genes are known to contain several and often very large introns, the number of introns in the divergent IRs was low (range: 0–3; Additional file 1: Table S1). Again, we observed fewer putatively functional IR genes and pseudogenes in the stenophagous species (Table 1).
Sensory neuron membrane proteins
We annotated four SNMP genes (all full-length models; Table 1) in each of D. ponderosae, A. planipennis and A. glabripennis. Both D. ponderosae and A. glabripennis have two members each of SNMP1 and SNMP2, whereas A. planipennis only has one member in each of these broadly conserved classes. The two remaining AplaSNMP genes encode proteins related to TcasSNMP3, and were thus named AplaSnmp3a and 3b. The beetle SNMP3 clade was positioned sister to the SNMP1/SNMP2 subfamilies (Fig. 4).
Odorant binding proteins
Our genome annotations revealed 36 OBPs in D. ponderosae and 12 OBPs in A. planipennis (all full-length models), which is fewer than in other species considered polyphagous (Table 1). Two of the DponOBP genes (DponObp37 and DponObp38) were exclusive to the male assembly, suggesting that they are located on the neoY chromosome. OBPs are classified into different groups based on the number of conserved cysteine (C) residues and their phylogenetic relationships [37, 68]. The “classic” OBPs share a characteristic pattern of six C residues. Members of the Minus-C class have lost two of these cysteines (generally C2 and C5), whereas the Plus-C OBPs typically have 12 conserved cysteines and a characteristic proline. Finally, one subfamily of the classic OBPs is further classified as “antennal binding protein II” (ABPII), members of which are generally upregulated in the antennae . The inspection of the patterns of C residues and our phylogenetic analysis showed that the genomes of D. ponderosae and A. planipennis contain one Plus-C member each, similar to other beetles (Fig. 5; Additional file 1: Table S1). Furthermore, 15 DponOBPs and four AplaOBPs presented the 4C pattern that is characteristic of the Minus-C group. However, two of these proteins (DponOBP13 and DponOBP22) did not group within the Minus-C clade in our phylogeny, but were placed with the classic OBPs with intermediate support (SH = 0.88). Seven DponOBPs and three AplaOBPs were placed within the ABPII clade (Fig. 5). In contrast to the polyphagous A. glabripennis and T. castaneum, no major species-specific lineage expansions were observed in the two stenophagous wood-borers apart from a small expansion of five Minus-C DponOBPs (DponOBP3, 9, 11, 28, 38).
Two of the DponOBP genes (DponObp6 and DponObp7) showed evidence of alternative splicing, supported by transcriptomic data [66, 69]. In both cases, the alternative splicing involves two mutually exclusive N-terminal exons encoding the short signal peptide, which appears to be alternatively combined with a shared C-terminal exon (DponObp7; Minus C-group) or six shared exons (DponObp6; ABPII group) (Additional file 1: Table S1). Finally, we identified an unusually large OBP in D. ponderosae (DponObp4), encoding 500 amino acids. Apart from a short N-terminal exon housing the signal peptide, this gene contains four similarly sized exons, each presenting the conserved Minus-C motif. This extraordinary Minus-C “tetramer” model was supported by previous transcriptomic data from this species [66, 69] and two other curculionids: the Yunnan pine shoot beetle (Tomicus yunnanensis; accession: GFJU01117056.1) and the red palm weevil (Rhynchophorus ferrugineus; GDKA01001723.1), retrieved from the Transcriptome Shotgun Assembly (TSA) collection at NCBI. The first three Minus-C exons of DponObp4 are separated by short, approx. 60 bp introns, whereas the final exon is separated by a 1.26 kb intron. To investigate how such a large OBP may have originated, we individually aligned the four Minus-C exons of DponOBP4 together with a subset of Minus-C OBPs, i.e., those encompassed under the most recent node shared by the individual DponOBP4 exons. The resulting phylogeny grouped DponOBP4 exons 2 and 3 together with moderate support, while exon 4 was positioned in a sister clade. Exon 5 was widely separated from the other exons but without support (Additional file 3: Figure S1). Inconsistent with this phylogeny, however, exon 2 and 4 shared the highest amino acid identity (41.6%), and a relatively high identity (30.7%) was shared between exon 3 and 5, suggesting that this protein may have originated from a dimer that underwent a duplication of its two major exons.
Total numbers of CSP genes were 11 (all full-length models) in D. ponderosae, 14 (13 full-length models) in A. planipennis, and 17 (16 full-length models) in A. glabripennis (Table 1). The majority of the beetle CSP genes are characterized by the presence of a single central intron in splice phase 1, however a few of the DponCSPs have one additional intron (phase 0) close to the N-terminus, with the first exon only coding for the first two amino acids of the protein. Most of the CSP genes within each species were assembled on the same genomic scaffold (Additional file 1: Table S1). The phylogenetic analysis revealed the presence of several highly-supported clades with one or two CSPs from each of the four species, suggesting the existence of several simple orthologous relationships in this gene family (Fig. 6). This includes a conserved clade of four CSPs (DponCSP12, AplaCSP8, AglaCSP3, and TcasCSP7E) with greatly elongated C-terminals and proteins ranging from 251 to 307 amino acids. Species-specific radiations of CSP lineages were rare, but a few smaller ones (comprising 3–6 CSPs) were evident in T. castaneum and A. planipennis. CSPs from the latter species were missing from a few well-supported clades that contained members from two or three of the other species.
We annotated the chemosensory gene families from the genomes of the two stenophagous wood-borers D. ponderosae and A. planipennis, and compared their gene repertoires with those in polyphagous species, in particular T. castaneum and A. glabripennis, with the latter species being a wood-borer recorded from > 100 host trees . Our annotations represent major qualitative and quantitative improvements of the original datasets derived from antennal transcriptomes [66, 67], with a large number of novel sequences identified and several original models extended to full length, corrected or discarded. With the present annotation of the CSPs and SNMPs also in the cerambycid A. glabripennis, the six main insect chemosensory gene families have now been identified from genomes of four species of Coleoptera [54, 56], whereas the chemoreceptors and OBPs have been annotated also in a fifth species, L. decemlineata . Hence, our study facilitates analysis of the molecular evolution of chemosensation in the largest order of insects, Coleoptera, and we can now begin to address questions of how beetle ecology relates to the diversification of these crucial gene families.
Correlation between chemosensory gene repertoire size and host range
The number of chemosensory genes varies between insect taxa due to different rates of gene gain (via tandem duplication) and loss (via pseudogenization and deletion). This variation is thought to relate to specific lifestyles and adaptations to the environment, and may ultimately result in speciation [2, 3, 5, 51, 70]. We predicted that host-specific insects would have fewer chemosensory genes than polyphagous species. Indeed, the total numbers of such genes in the Pinus specialist D. ponderosae and the Fraxinus specialist A. planipennis are lower than in T. castaneum and A. glabripennis (Table 1). This was consistent across all chemosensory gene families (except the SNMPs), with especially large differences observed among the ORs, GRs, and IRs, followed by the OBPs, especially in A. planipennis. The smaller gene numbers in stenophagous species are largely explained by reductions in the extent and number of paralogous radiations in the two specialized wood-borers. Their lower numbers of GRs are at least partly manifested through low frequency of alternative splicing in both D. ponderosae and A. planipennis. In fact, none of the GR genes in the latter species showed indications of alternative splicing, which is unique among the coleopterans investigated so far. In D. ponderosae, seven GR genes are putatively alternatively spliced, but with only two or four splice variants per gene, comprising 18% of the total GRs. Though only 13% of the GRs of T. castaneum are splice variants, 30 splice variants are encoded by the single gene TcasGr214 . In A. glabripennis , 39% of the GRs are splice variants with up to 17 proteins from a single gene (AglaGr99). The same percentage is found in the oligophagous Colorado potato beetle, L. decemlineata, which feeds on a variety of solanaceous plants, with up to 13 splice variants encoded by one gene (LdecGr48) . However, compared to the two polyphagous species, L. decemlineata also has fewer chemoreceptors, especially a highly reduced IR repertoire, and the size of its OR repertoire is similar to that in D. ponderosae. D. ponderosae and A. planipennis also have proportionally fewer pseudogenes than A. glabripennis and T. castaneum, especially among the GRs. This suggests that the smaller complements of chemoreceptors are due to reduced gene gain via duplication rather than increased gene loss via pseudogenization.
While the observations across these five beetle species suggest a correlation between the number of chemosensory genes and diet breadth, additional species need to be sampled to draw general conclusions, because other ecological factors and the species phylogeny may also play a role. For instance, A. planipennis has well-developed eyes, and visual stimuli are important for mate and host attraction [63, 71], which might correlate with reduced reliance on chemosensation. This hypothesis is in line with our results, with this species having the lowest number of chemosensory genes, including to our knowledge the fewest OBPs recorded from a holometabolous insect. Secondly, our sampling included single representatives from four families (Apla: Buprestidae, Tcas: Tenebrionidae, Agla: Cerambycidae, and Dpon: Curculionidae) unevenly distributed across the coleopteran phylogeny, which may have influenced the outcome of the analysis. A. planipennis is part of the relatively distant infraorder Elateriformia, whereas the other species belong to the “higher beetles”, Cucujiformia . Indeed, previous antennal transcriptome studies of species from early diverging lepidopteran lineages and caddisflies (sister taxon to the Lepidoptera) suggest a smaller number of ORs as compared to species belonging to more recent lineages of the Lepidoptera [73,74,75]. However, no such phylogenetic trend is clear for the ORs in Coleoptera . Additionally, the polyphagous cerambycid A. glabripennis and the oligophagous chrysomelid L. decemlineata belong to the same superfamily (Chrysomeloidea), which is sister to the superfamily of the stenophagous D. ponderosae (Curculionoidea) . Hence, restricting the comparison to these three relatively related species suggests an increasing number of chemosensory genes with a broader host range. Similarly, larger repertoires of ORs and GRs in the Culex quinquefasciatus mosquito is thought to be attributed to its broader host range (humans, livestock and birds) as compared to the more host-specific mosquitos Anopheles gambiae and Aedes aegypti . Moreover, in drosophilids, the specialist D. sechellia is losing OR and GR genes at a faster pace than its generalist congeners D. melanogaster and D. simulans . On the other hand, the sizes of the chemoreceptor gene repertoires are relatively conserved across sixteen species of Anopheles species irrespective of host range, suggesting that correlations between chemoreceptor repertoires and host range are not always evident, at least not among congeners .
Evolutionary divergence and conservation of chemosensory genes
Larger gene-lineage expansions in D. ponderosae and A. planipennis are restricted to the three receptor gene families, and simple orthologous relationships among receptors were generally rare. In the present comparison with the polyphagous species, the positions in the trees of the expansions appear to be “arbitrary”, but may possibly reflect differences in ecological traits, such as feeding from conifers (D. ponderosae), angiosperms (A. planipennis and A. glabripennis), or stored products (T. castaneum). However, an analysis of the ORs from ten beetle genomes suggests that the distribution of ORs across the tree is at least partly dictated by the coleopteran phylogeny, with more similar distributions observed in closely compared to distantly related taxa . This in turn implies that convergent evolution is an important driver for the function of ORs, since many compounds, such as green leaf volatiles, are detected by beetle species from a variety of families [64, 79, 80]. Similar to other bark beetles and weevils [66, 81, 82], the largest expansions of ORs in D. ponderosae are found within subfamily 7, followed by 5A and 1. Interestingly, D. ponderosae appears to have lost ORs from several subfamilies that are largely conserved across the Coleoptera. Especially noteworthy are subfamilies 3 and 5B, with the former housing relatively large proportions of the Apla-, Agla-, Tcas-, and LdecORs, and the latter containing one or a few ORs from most beetles investigated so far . Functional data from ORs of these two subfamilies are needed to better understand whether or not the losses observed in D. ponderosae are related to its ecology. In contrast, A. planipennis has no ORs in subfamilies 7, 5A, or 1, and most of its ORs belong to subfamily 2B, which is highly reduced in D. ponderosae. However, a noteworthy common denominator in the two stenophagous wood-borers is their lack of ORs in subfamily 4, which is unique among the beetles investigated so far . Whether this relates to ecological similarities, or if it is a coincidence remains unknown.
Conserved GRs for carbon dioxide were found in both stenophagous wood-borers, but GR1 was not recovered from the A. planipennis assembly or from the raw sequence data. While drosophilids lack GR2 and still detect carbon dioxide , it is unclear whether the apparent lack of GR1 means that A. planipennis has lost this ability, or if the AplaGr1 gene simply was missed in the sequencing of A. planipennis. Both genomes contained genes for six putative sugar receptors (GR4–9) and one fructose receptor (GR10), which is fewer than in the two polyphagous species and might relate to their narrow host ranges. Reduction in candidate sugar receptor lineages was previously observed in the gall-midge Mayetiola destructor (Diptera), which is specialized on wheat and a few other grasses . Similar to the ORs, putative bitter taste GRs were primarily distributed in species-specific lineage expansions at different positions in the phylogeny, with very few orthologuous relationships observed. The only orthologous clade was termed the “GR215” clade (following the orthologue from T. castaneum), and functional studies of these receptors are warranted to shed light on this conservation. Notably, A. planipennis is missing GRs from a major clade of our tree, suggesting a loss of GRs in this species, or that these GRs originated in more recent coleopteran lineages.
Although we identified fewer IRs in the stenophagous compared to the polyphagous species, the reduction was not as large as for the ORs and GRs. All members of the conserved olfactory antennal IRs  and members of the divergent IR100a clade were present in both D. ponderosae and A. planipennis. Duplications have occurred for DponIr41a and AplaIr76b, which is also the case in several species of Lepidoptera . Furthermore, our analysis suggests the existence of orthologues of DmelIr60a in all beetle species. This orthologue is also conserved in moths and caddisflies, demonstrating a broad occurrence of this receptor in insects . Surprisingly, the genome of D. ponderosae encoded more IR75 members (11) than any other beetle genome. IR75 paralogs have been implicated in olfaction and/or taste in moths based on expression patterns , and underlie responses to propionic acid in D. melanogaster . Functional characterization will be necessary to confirm their putative olfactory roles in D. ponderosae.
We revealed four SNMPs in each of the three wood-boring species, which included two members in A. planipennis that are related to TcasSNMP3 . The absence of SNMP3 in several beetle species suggests that its taxonomic occurrence is restricted. T. castaneum has a total of six SNMPs, and is only exceeded by M. destructor in terms of genomic content of SNMP genes . While SNMP1 is important for pheromone responses in some species , the roles of different SNMP1 paralogues , SNMP2, and SNMP3 remain unknown, although transcripts of TcasSNMP3 are enriched in antennae and mouthparts, which suggests a putative role in chemosensation .
The OBP and CSP gene families are devoid of large species-specific radiations in D. ponderosae and A. planipennis, and the OBP family is especially reduced in both species. In contrast to the receptors, several orthologous clades with proteins from all four species were present in both gene families, suggesting that the evolutionary forces acting on these genes are different as compared to those acting on the receptor genes. The DponOBP family also exhibited alternative splicing and a “tetramer” gene form, neither of which have been previously reported from insects. Two genes (DponObp6a/b and DponObp7a/b) alternated a first exon that encoded the signal peptide, a hydrophobic motif that allows excretion of the protein from the cell. Previous transcriptome data indicate that DponObp6 is antenna-specific, whereas DponObp7 is expressed in a variety of tissues, but not in the antenna (see Additional file 3: Figure S1 in ). Hence, a speculative adaptive value of tissue-specific signal peptide composition may be to facilitate OBP excretion from cells that may differ in phospholipid membrane composition. The tetramer, DponOBP4, consists of a signal peptide followed by four consecutive exons, each of which is homologous to a member of the “Minus-C” subfamily of OBPs. Similar gene forms have been found previously, but only as pairs of exons (“dimers”) , including a Minus-C dimer in L. decemlineata . While there are multiple evolutionary scenarios that could explain the origin of this unusual gene, the most parsimonious mechanism would be that the two “Minus-C” exons of an original dimer duplicated and were subsequently joined to the same gene as the parental exons. Alternatively, a duplicated complete dimer gene may have lost its short N-terminal exon, and the two main exons have then joined the upstream dimer. Unfortunately, our phylogenetic analysis did not conclusively demonstrate which scenario is the most plausible, although the comparatively high amino acid identity between exons 2 and 4 suggests a duplication event has been involved. The analysis also suggests that the sequences of the four exons have diverged significantly and that the duplication event hence must be ancient, which is supported by apparent orthologues in other curculionids. DponObp4 is not expressed in the adult antennae (see Additional file 3: Figure S1 in ), which is similar to the dimer OBP in D. melanogaster , and suggests that OBP dimers and tetramers may not be involved in olfaction.
Our genomic annotation of the chemosensory gene families in three species of wood-boring beetles includes a large number of novel sequences and significant improvements of original datasets obtained from transcriptomes. Hence, this study is an important contribution to the known chemosensory genes in the Coleoptera, facilitating evolutionary analysis. The results suggest a correlation between host range and numbers of chemosensory genes in essentially all chemosensory gene families (i.e., ORs, GRs, IRs, OBPs, and CSPs), with reductions in stenophagous beetle species largely explained by limited lineage expansions in most gene families (including both putatively functional genes and pseudogenes), entire lineage losses, and less alternative splicing of GR genes. The reductions in gene numbers may be further augmented in A. planipennis due to its well-developed visual system. Our phylogenetic analysis shows that orthology is generally rare among chemoreceptors from different beetle families, but we also reveal conservation of IR60a across beetles, and a conserved GR clade that we named the GR215 clade. In D. ponderosae we suggest alternative splicing of two of its OR genes, and we also provide the first evidence of alternative splicing among the OBP genes and a novel OBP Minus-C tetramer. Expansion of the IR75 clade in D. ponderosae is interesting in relation to its specialized ecology and destructive potential as a pest. This study provides an important platform for future functional characterization of chemoreceptors and binding proteins, which is a crucial step towards improved control of these economically devastating forest pests .
Annotation of chemosensory genes
Exhaustive tBLASTn searches against the genome assemblies of D. ponderosae (Curculionidae), A. planipennis (Buprestidae), and A. glabripennis (Cerambycidae) were conducted to identify chemosensory genes, using query sequences from I. typographus, D. ponderosae, T. castaneum, A. glabripennis, and L. decemlineata [33, 49, 54, 56, 66]. Annotation in D. ponderosae was conducted using primarily the published assembly of the female genome (accession APGL01000000) . The current male assembly (APGK01000000) is more fragmented and was only used, when possible, to complete or improve those gene models that presented gaps in the female assembly. BLAST searches against the male assembly were also undertaken to ensure that no genes had been missed in the annotation of the female genome, which identified two OBPs exclusively found in the male. The chemosensory genes of A. planipennis were annotated from a public genome assembly (PRJNA230921) made available through the i5K project, and were used with permission. The CSP and SNMP genes from A. glabripennis were annotated using the published genome assembly (GCA_000390285.2) . An e-value of cut-off at 10− 1 was used in the BLAST searches except for the putative bitter-taste GRs and divergent IRs where a cut-off at 3 (or occasionally 10) was used to ensure that divergent sequences were not missed. HMMER 3.1b1 (Eddy SR, Wheeler TJ, & HMMER development team, HMMER: biosequence analysis using profile hidden Markov models. http://hmmer.org/) with Pfam profiles PBP_GOBP (PF01395) and OS-D (PF03392) were also used to identify potential OBPs and CSPs from the NCBI REFSEQ sequences. The protein sequences of all identified genes were then blasted against each respective genome until the annotation of all novel hits was completed. Gene annotations and determination of exon/intron structure were performed manually using Geneious software package 7.1.9. (Biomatters Ltd., Auckland, New Zealand) or CLC Main Workbench 8.1 (QIAGEN Aarhus A/S, Denmark). Scaffold locations and additional annotation details for all genes are reported in Additional file 1: Table S1. Amino acid sequences for all annotations in this study are present in Additional file 4.
Following established nomenclature guidelines, two criteria were considered for the naming of the ORs, most GRs, and the ‘divergent’ IRs. First, genes in tandem arrays on scaffolds were given consecutive numbers. Secondly, preliminary trees were constructed to assign numbers to genes following their phylogenetic position, which for tandemly duplicated genes corresponded to their scaffold locations (for additional details see ). Hence, several of the previously identified genes were assigned new numbers in the present study (see Additional file 2: Table S2 for correspondence). Conserved GRs for carbon dioxide were named GR1-GR3 [according to 22], non-fructose sugar receptors GR4-GR9, and one putative fructose receptor in D. ponderosae and A. planipennis was named GR10 in both species. The naming scheme for the remaining, putative bitter-taste GRs, followed the two criteria described for the ORs with consecutive numbering starting at GR11. The consecutive numbering of the divergent IRs started at IR101 to avoid implying orthology to unrelated IRs in D. melanogaster. Conserved antennal IRs and all SNMPs were named according to their orthologous relationships with proteins in D. melanogaster and T. castaneum [23, 31, 33]. With the exception of IR75e, the other IR75 members did not show simple orthologous relationships across beetles, and they were given the consecutive letter suffixes “a-d” and “f-k”. The previously identified CSPs and OBPs in D. ponderosae and A. planipennis [66, 67] that were confirmed in the present study retained their original names, since no consensus in the nomenclature exists for these families. The CSPs in A. glabripennis annotated here were named consecutively according to scaffold positions. Exons of OR genes were named exon A, B, C, D, and E following ancestral splicing patterns , where the N-terminal A exon frequently is divided by one to several introns (in these instances, exons were named A1, A2, A3, …, A#X, according to ). No specific exon nomenclature was employed for the other gene families.
Models of chemosensory genes were frequently incomplete due to assembly gaps, large introns, or small and/or highly divergent terminal exons. Hence, we added suffixes to these gene names according to established protocols [87, 88]. Proteins with missing or truncated N-terminal, C-terminal, or internal exons were given the suffixes NTE, CTE, and INT, respectively (but partial models from T. castaneum retained their PAR suffix as previously reported). Gene models split across scaffolds were given a JOI suffix, which were confirmed by transcriptomic data when available [66, 69]. Models extended or corrected using raw genomic and transcriptomic read data were noted as FIX. A PSE suffix was added to models regarded as putative pseudogenes, including genes containing premature stop codon(s), frameshifts, missing start codon, exon(s), and/or splice sites. For models with more than one suffix, one-letter suffix abbreviations were used in relevant combinations (i.e., N, C, I, J, F, P). For several of the GRs, two ORs and two OBPs in D. ponderosae, we found indications of alternative splicing with mutually-exclusive N-terminal exons assembled consecutively with one or several seemingly shared C-terminal exons. Potential splice variants were named as e.g., GR#a, GR#b, GR#c etc. by convention . Highly degenerated pseudogenes or fragments of putatively functional genes corresponding to single or a few short exons were discarded to improve alignments and phylogenetic analysis, and to avoid over-estimation of gene counts.
Multiple sequence alignments of protein sequences were performed using MAFFT v 7.017 , implemented in Geneious software package v7.1.9. The alignments were manually adjusted when necessary to correctly align some of the partial protein sequences. Uninformative regions were excised using trimAl v1.2  with the following settings: similarity threshold 0, gap threshold 0.7, minimum 25% of conserved positions. The trimmed alignments of GR, IR, SNMP, OBP, and CSP proteins were used to construct trees using FastTree v2.1.10, at default settings . Local branch support values were calculated using the Shimodaira-Hasegawa (SH) test implemented within FastTree. However, when constructing preliminary OR trees, FastTree performed inconsistently, with the tree topology affected by minor adjustments to the alignment and the inclusion/exclusion of individual ORs. Hence, we instead employed PhyML 3.0  to perform phylogenetic analysis for this gene family, which also facilitated direct comparisons with the across-Coleoptera OR trees recently constructed using this method . Prior to tree construction, PartitionFinder 2  was used to select a model of gene evolution, with the best fit obtained for a JTT amino acid substitution matrix, gamma distributed rate variation, and empirical equilibrium amino acid frequencies (JTT + G + F). Branch support was calculated by aLRT (approximate Likelihood Ratio Test) SH-like likelihood ratios, implemented in PhyML. Trees were visualized and color coded in FigTree v1.4.3 , and graphically adjusted in Adobe Illustrator and Corel PaintShop Pro 2018 (Corel Corporation, Ottawa, Canada). Pseudogenes from A. glabripennis and T. castaneum were excluded from the phylogenetic analyses of all chemosensory gene families.
The OR sequences from D. ponderosae and A. planipennis were analyzed together with those from A. glabripennis  and T. castaneum (Tenebrionidae). The OR set used for the latter species was an updated version of the original dataset , which included 22 revised gene models . Partial OR sequences below 200 amino acids were excluded from the analysis, except for DponOR53INT (195 amino acids) in order to include all ORs from this study-species. To improve the legibility of the tree, the two massively expanded and species-specific lineages of TcasORs, originally classified as OR subfamilies 5 and 6 , were each represented by 10 TcasORs. The phylogenetic analysis of the GR family included the same species as the OR analysis. GR sequences from A. glabripennis were retrieved from McKenna et al. , and T. castaneum GRs from Tribolium genome sequencing consortium . Due to the highly divergent nature of this receptor family, sequences below 250 amino acids were excluded to allow for a more robust phylogenetic analysis. The analysis of the IRs proceeded similarly, but also included the IRs from L. decemlineata (Chrysomelidae)  to facilitate inferences of orthologuous relationships across beetles. Sequences were derived from the same sources as described above, apart from the TcasIRs for which we used a revised dataset . We also included the widely-conserved antennal IR orthologues from D. melanogaster (Diptera)  to support our naming of conserved IRs in the beetles and to infer across-order conservation. The SNMP analysis included the same species as the OR and GR trees, but also D. melanogaster. Sequences of SNMPs from T. castaneum were retrieved from Dippel et al. , and D. melanogaster SNMPs from GenBank (accession numbers: ABW70129.1 and NP_650953.1). Croquemort proteins, which are non-SNMP members of the CD36 family, from D. melanogaster and T. castaneum (accession numbers NP_787957.1 and XP_008192356.1), were included to root the tree. The OBP and CSP analyses included sequences from D. ponderosae, A. planipennis, A. glabripennis, and T. castaneum. OBPs from A. glabripennis were retrieved from Wang et al. , and OBPs and CSPs from T. castaneum from Dippel et al. .
Availability of data and materials
All data generated or analyzed during this study are included in this published article and its supplementary information files. GenBank accession numbers for genome assemblies: D. ponderosae female APGL01000000; D. ponderosae male APGK01000000; A. glabripennis GCA_000390285.2; A. planipennis PRJNA230921. BLAST query sequences for D. ponderosae and I. typographus were retrieved from the Transcriptome Shotgun Assembly (TSA) database (accession numbers GABX00000000 and GACR00000000), and query sequences from T. castaneum, A. glabripennis and L. decemlineata were retrieved from previous publications (refs [33, 49, 54, 56, 57]).
Ionotropic glutamate receptor
Odorant binding protein
Olfactory sensory neuron
Sensory neuron membrane protein
Hansson BS, Stensmyr MC. Evolution of insect olfaction. Neuron. 2011;72:698–711.
Benton R. Multigene family evolution: perspectives from insect chemoreceptors. Trends Ecol Evol. 2015;30:590–600.
Nei M, Niimura Y, Nozawa M. The evolution of animal chemosensory receptor gene repertoires: roles of chance and necessity. Nat Rev Genet. 2008;9:951–63.
Andersson MN, Newcomb RD. Pest control compounds targeting insect chemoreceptors: another silent spring? Front Ecol Evol. 2017;5:5.
Andersson MN, Löfstedt C, Newcomb RD. Insect olfaction and the evolution of receptor tuning. Front Ecol Evol. 2015;3:53.
Benton R, Sachse S, Michnick SW, Vosshall LB. Atypical membrane topology and heteromeric function of Drosophila odorant receptors in vivo. PLoS Biol. 2006;4:240–57.
Clyne PJ, Warr CG, Freeman MR, Lessing D, Kim J, Carlson JR. A novel family of divergent seven-transmembrane proteins: candidate odorant receptors in Drosophila. Neuron. 1999;22:327–38.
Gao Q, Chess A. Identification of candidate Drosophila olfactory receptors from genomic DNA sequence. Genomics. 1999;60:31–9.
Vosshall L, Amrein H, Morozov P, Rzhetsky A, Axel R. A spatial map of olfactory receptor expression in the Drosophila antenna. Cell. 1999;96:725–36.
Smart R, Kiely A, Beale M, Vargas E, Carraher C, Kralicek AV, et al. Drosophila odorant receptors are novel seven transmembrane domain proteins that can signal independently of heterotrimeric G proteins. Insect Biochem Mol Biol. 2008;38:770–80.
Vosshall LB, Hansson BS. A unified nomenclature system for the insect olfactory coreceptor. Chem Senses. 2011;36:497–8.
Brand P, Robertson HM, Lin W, Pothula R, Klingeman WE, Jurat-Fuentes JL, et al. The origin of the odorant receptor gene family in insects. eLife. 2018;7:e38340.
Butterwick JA, del Mármol J, Kim KH, Kahlson MA, Rogow JA, Walz T, et al. Cryo-EM structure of the insect olfactory receptor Orco. Nature. 2018;560:447–52.
Larsson MC, Domingos AI, Jones WD, Chiappe ME, Amrein H, Vosshall LB. Or83b encodes a broadly expressed odorant receptor essential for Drosophila olfaction. Neuron. 2004;43:703–14.
Sato K, Pellegrino M, Nakagawa T, Vosshall LB, Touhara K. Insect olfactory receptors are heteromeric ligand-gated ion channels. Nature. 2008;452:1002–6.
Wicher D, Schäfer R, Bauernfeind R, Stensmyr MC, Heller R, Heinemann SH, et al. Drosophila odorant receptors are both ligand-gated and cyclic-nucleotide-activated cation channels. Nature. 2008;452:1007–11.
Clyne PJ, Warr CG, Carlson JR. Candidate taste receptors in Drosophila. Science. 2000;287:1830–4.
Vosshall LB, Stocker RF. Molecular architecture of smell and taste in Drosophila. Annu Rev Neurosci. 2007;30:505–33.
S-i E, Soh HY, Posavi M, Munro JB, Hughes DS, Murali SC, et al. Evolutionary history of chemosensory-related gene families across the Arthropoda. Mol Biol Evol. 2017;34:1838–62.
Robertson HM, Warr CG, Carlson JR. Molecular evolution of the insect chemoreceptor gene superfamily in Drosophila melanogaster. Proc Natl Acad Sci U S A. 2003;100:14537–42.
Kwon JY, Dahanukar A, Weiss LA, Carlson JR. The molecular basis of CO2 reception in Drosophila. Proc Natl Acad Sci. 2007;104:3574–8.
Robertson HM, Kent LB. Evolution of the gene lineage encoding the carbon dioxide receptor in insects. J Insect Sci. 2009;9:19.
Benton R, Vannice KS, Gomez-Diaz C, Vosshall LB. Variant ionotropic glutamate receptors as chemosensory receptors in Drosophila. Cell. 2009;136:149–62.
Abuin L, Bargeton B, Ulbrich MH, Isacoff EY, Kellenberger S, Benton R. Functional architecture of olfactory ionotropic glutamate receptors. Neuron. 2011;69:44–60.
Croset V, Rytz R, Cummins SF, Budd A, Brawand D, Kaessmann H, et al. Ancient protostome origin of chemosensory ionotropic glutamate receptors and the evolution of insect taste and olfaction. PLoS Genet. 2010;6:e1001064.
Rytz R, Croset V, Benton R. Ionotropic Receptors (IRs). Chemosensory ionotropic glutamate receptors in Drosophila and beyond. Insect Biochem Mol Biol. 2013;43:888–97.
Chen C, Buhl E, Xu M, Croset V, Rees JS, Lilley KS, et al. Drosophila ionotropic receptor 25a mediates circadian clock resetting by temperature. Nature. 2015;527:516–20.
Enjin A, Zaharieva EE, Frank DD, Mansourian S, Suh GS, Gallio M, et al. Humidity sensing in Drosophila. Curr Biol. 2016;26:1352–8.
Zhang YV, Ni J, Montell C. The molecular basis for attractive salt-taste coding in Drosophila. Science. 2013;340:1334–8.
Sánchez-Alcañiz JA, Silbering AF, Croset V, Zappia G, Sivasubramaniam AK, Abuin L, et al. An expression atlas of variant ionotropic glutamate receptors identifies a molecular basis of carbonation sensing. Nat Commun. 2018;9:4252.
Benton R, Vannice KS, Vosshall LB. An essential role for a CD36-related receptor in pheromone detection in Drosophila. Nature. 2007;450:289–93.
Nichols Z, Vogt RG. The SNMP/CD36 gene family in Diptera, Hymenoptera and Coleoptera: Drosophila melanogaster, D. pseudoobscura, Anopheles gambiae, Aedes aegypti, Apis mellifera, and Tribolium castaneum. Insect Biochem Mol Biol. 2008;38:398–415.
Dippel S, Kollmann M, Oberhofer G, Montino A, Knoll C, Krala M, et al. Morphological and transcriptomic analysis of a beetle chemosensory system reveals a gnathal olfactory center. BMC Biol. 2016;14:90.
Gomez-Diaz C, Bargeton B, Abuin L, Bukar N, Reina JH, Bartoi T, et al. A CD36 ectodomain mediates insect pheromone detection via a putative tunnelling mechanism. Nat Commun. 2016;7:11866.
Li Z, Ni JD, Huang J, Montell C. Requirement for Drosophila SNMP1 for rapid activation and termination of pheromone-induced activity. PLoS Genet. 2014;10:e1004600.
Pregitzer P, Greschista M, Breer H, Krieger J. The sensory neurone membrane protein SNMP1 contributes to the sensitivity of a pheromone detection system. Insect Mol Biol. 2014;23:733–42.
Sánchez-Gracia A, Vieira FG, Rozas J. Molecular evolution of the major chemosensory gene families in insects. Heredity. 2009;103:208–16.
Vogt RG, Prestwich GD, Lerner MR. Odorant-binding-protein subfamilies associate with distinct classes of olfactory receptor neurons in insects. J Neurobiol. 1991;22:74–84.
Große-Wilde E, Svatoš A, Krieger J. A pheromone-binding protein mediates the bombykol-induced activation of a pheromone receptor in vitro. Chem Senses. 2006;31:547–55.
Leal WS. Odorant reception in insects. Roles of receptors, binding proteins, and degrading enzymes. Annu Rev Entomol. 2013;58:373–91.
Damberger FF, Michel E, Ishida Y, Leal WS, Wüthrich K. Pheromone discrimination by a pH-tuned polymorphism of the Bombyx mori pheromone-binding protein. Proc Natl Acad Sci U S A. 2013;110:18680–5.
Larter NK, Sun JS, Carlson JR. Organization and function of Drosophila odorant binding proteins. eLife. 2016;5:e20242.
Li Z, Dai L, Chu H, Fu D, Sun Y, Chen H. Identification, expression patterns, and functional characterization of chemosensory proteins in Dendroctonus armandi (Coleoptera: Curculionidae: Scolytinae). Front Physiol. 2018;9:291.
Bohbot J, Sobrio F, Lucas P, Nagnan-Le MP. Functional characterization of a new class of odorant-binding proteins in the moth Mamestra brassicae. Biochem Biophys Res Commun. 1998;253:489–94.
Pelosi P, Zhou JJ, Ban LP, Calvello M. Soluble proteins in insect chemical communication. Cell Mol Life Sci. 2006;63:1658–76.
Kitabayashi AN, Arai T, Kubo T, Natori S. Molecular cloning of cDNA for p10, a novel protein that increases in the regenerating legs of Periplaneta americana (American cockroach). Insect Biochem Mol Biol. 1998;28:785–90.
Montagné N, de Fouchier A, Newcomb RD, Jacquin-Joly E. Advances in the identification and characterization of olfactory receptors in insects. Prog Mol Biol Transl Sci. 2015;130:55–80.
Liu Y, Sun L, Cao D, Walker WB, Zhang Y, Wang G. Identification of candidate olfactory genes in Leptinotarsa decemlineata by antennal transcriptome analysis. Front Ecol Evol. 2015;3:60.
Schoville SD, Chen YH, Andersson MN, Benoit JB, Bhandari A, Bowsher JH, et al. A model species for agricultural pest genomics: the genome of the Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae). Sci Rep. 2018;8:1931.
Engsontia P, Sangket U, Chotigeat W, Satasook C. Molecular evolution of the odorant and gustatory receptor genes in lepidopteran insects: implications for their adaptation and speciation. J Mol Evol. 2014;79:21–39.
Liu N-Y, Xu W, Dong S-L, Zhu J-Y, Xu Y-X, Anderson A. Genome-wide analysis of ionotropic receptor gene repertoire in Lepidoptera with an emphasis on its functions of Helicoverpa armigera. Insect Biochem Mol Biol. 2018;99:37–53.
Zhao C, Escalante LN, Chen H, Benatti TR, Qu J, Chellapilla S, et al. A massive expansion of effector genes underlies gall-formation in the wheat pest Mayetiola destructor. Curr Biol. 2015;25:613–20.
Mitchell RF, Schneider TM, Schwartz AM, Andersson MN, McKenna DD. The diversity and evolution of odorant receptors in beetles (Coleoptera). Insect Mol Biol. 2019. https://doi.org/10.1111/imb.12611.
Engsontia P, Sanderson AP, Cobb M, Walden KKO, Robertson HM, Brown S. The red flour beetle's large nose: an expanded odorant receptor gene family in Tribolium castaneum. Insect Biochem Mol Biol. 2008;38:387–97.
Tribolium genome sequencing consortium. The genome of the model beetle and pest Tribolium castaneum. Nature 2008;452:949–955.
McKenna DD, Scully ED, Pauchet Y, Hoover K, Kirsch R, Geib SM, et al. Genome of the Asian longhorned beetle (Anoplophora glabripennis), a globally significant invasive species, reveals key functional and evolutionary innovations at the beetle–plant interface. Genome Biol. 2016;17:227.
Dippel S, Oberhofer G, Kahnt J, Gerischer L, Opitz L, Schachtner J, et al. Tissue-specific transcriptomics, chromosomal localization, and phylogeny of chemosensory and odorant binding proteins from the red flour beetle Tribolium castaneum reveal subgroup specificities for olfaction or more general functions. BMC Genomics. 2014;15:1141.
Meng P, Hoover K, Keena M. Asian longhorned beetle (Coleoptera: Cerambycidae), an introduced pest of maple and other hardwood trees in North America and Europe. J Integr Pest Manag. 2015;6:4.
Raffa KF, Andersson MN, Schlyter F. Chapter one-Host selection by bark beetles: Playing the odds in a high-stakes game. In: Tittiger C, Blomquist GJ, editors. Adv Insect Physiol, vol. 50. Oxford: Academic press; 2016. p. 1–74.
Conn J, Borden J, Scott B, Friskie L, Pierce H Jr, Oehlschlager A. Semiochemicals for the mountain pine beetle, Dendroctonus ponderosae (Coleoptera: Scolytidae) in British Columbia: field trapping studies. Can J For Res. 1983;13:320–4.
Kurz WA, Dymond CC, Stinson G, Rampley GJ, Neilson ET, Carroll AL, et al. Mountain pine beetle and forest carbon feedback to climate change. Nature. 2008;452:987–90.
Anulewicz AC, Mccullough DG, Cappaert DL, Poland TM. Host range of the emerald ash borer (Agrilus planipennis Fairmaire) (Coleoptera: Buprestidae) in North America: results of multiple-choice field experiments. Environ Entomol. 2014;37:230–41.
Crook DJ, Mastro VC. Chemical ecology of the emerald ash borer Agrilus planipennis. J Chem Ecol. 2010;36:101–12.
de Groot P, Grant GG, Poland TM, Scharbach R, Buchan L, Nott RW, et al. Electrophysiological response and attraction of emerald ash borer to green leaf volatiles (GLVs) emitted by host foliage. J Chem Ecol. 2008;34:1170–9.
Pureswaran DS, Poland TM. The role of olfactory cues in short-range mate finding by the emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae). J Insect Behav. 2009;22:205–16.
Andersson MN, Grosse-Wilde E, Keeling CI, Bengtsson JM, Yuen MM, Li M, et al. Antennal transcriptome analysis of the chemosensory gene families in the tree killing bark beetles, Ips typographus and Dendroctonus ponderosae (Coleoptera: Curculionidae: Scolytinae). BMC Genomics. 2013;14:198.
Mamidala P, Wijeratne AJ, Wijeratne S, Poland T, Qazi SS, Doucet D, et al. Identification of odor-processing genes in the emerald ash borer, Agrilus planipennis. PloS one. 2013;8:e56555.
Hekmat-Scafe DS, Scafe CR, McKinney AJ, Tanouye MA. Genome-wide analysis of the odorant-binding protein gene family in Drosophila melanogaster. Genome Res. 2002;12:1357–69.
Keeling CI, Henderson H, Li M, Yuen M, Clark EL, Fraser JD, et al. Transcriptome and full-length cDNA resources for the mountain pine beetle, Dendroctonus ponderosae Hopkins, a major insect pest of pine forests. Insect Biochem Mol Biol. 2012;42:525–36.
Smadja C, Shi P, Butlin RK, Robertson HM. Large gene family expansions and adaptive evolution for odorant and gustatory receptors in the pea aphid, Acyrthosiphon pisum. Mol Biol Evol. 2009;26:2073–86.
Sharkey CR, Fujimoto MS, Lord NP, Shin S, McKenna DD, Suvorov A, et al. Overcoming the loss of blue sensitivity through opsin duplication in the largest animal group, beetles. Sci Rep. 2017;7:8.
Hunt T, Bergsten J, Levkanicova Z, Papadopoulou A. St. John O, wild R, et al. a comprehensive phylogeny of beetles reveals the evolutionary origins of a superradiation. Science. 2007;318:1913–6.
Yuvaraj JK, Andersson MN, Zhang D-D, Löfstedt C. Antennal transcriptome analysis of the chemosensory gene families from Trichoptera and basal Lepidoptera. Front Physiol. 2018;9:1365.
Yuvaraj JK, Corcoran JA, Andersson MN, Newcomb RD, Anderbrant O, Löfstedt C. Characterization of odorant receptors from a non-ditrysian moth, Eriocrania semipurpurella sheds light on the origin of sex pheromone receptors in Lepidoptera. Mol Biol Evol. 2017;34:2733–46.
Yuvaraj JK, Andersson MN, Corcoran JA, Anderbrant O, Löfstedt C. Functional characterization of odorant receptors from Lampronia capitella suggests a non-ditrysian origin of the lepidopteran pheromone receptor clade. Insect Biochem Mol Biol. 2018;100:39–47.
Arensburger P, Megy K, Waterhouse RM, Abrudan J, Amedeo P, Antelo B, et al. Sequencing of Culex quinquefasciatus establishes a platform for mosquito comparative genomics. Science. 2010;330:86–8.
McBride CS. Rapid evolution of smell and taste receptor genes during host specialization in Drosophila sechellia. Proc Natl Acad Sci. 2007;104:4996–5001.
Neafsey DE, Waterhouse RM, Abai MR, Aganezov SS, Alekseyev MA, Allen JE, et al. Highly evolvable malaria vectors: the genomes of 16 Anopheles mosquitoes. Science. 2015;347:1258522.
Andersson MN, Larsson MC, Schlyter F. Specificity and redundancy in the olfactory system of the bark beetle Ips typographus: single-cell responses to ecologically relevant odors. J Insect Physiol. 2009;55:556–67.
Larsson MC, Leal WS, Hansson BS. Olfactory receptor neurons detecting plant odours and male volatiles in Anomala cuprea beetles (Coleoptera: Scarabaeidae). J Insect Physiol. 2001;47:1065–76.
Antony B, Soffan A, Jakše J, Abdelazim MM, Aldosari SA, Aldawood AS, et al. Identification of the genes involved in odorant reception and detection in the palm weevil Rhynchophorus ferrugineus, an important quarantine pest, by antennal transcriptome analysis. BMC Genomics. 2016;17:69.
Gu X-C, Zhang Y-N, Kang K, Dong S-L, Zhang L-W. Antennal transcriptome analysis of odorant reception genes in the red turpentine beetle (RTB), Dendroctonus valens. PloS one. 2015;10:e0125159.
Andersson MN, Videvall E, Walden KKO, Harris MO, Robertson HM, Löfstedt C. Sex- and tissue-specific profiles of chemosensory gene expression in a herbivorous gall-inducing fly (Diptera: Cecidomyiidae). BMC Genomics. 2014;15:501.
Andersson MN, Corcoran JA, Zhang D-D, Hillbur Y, Newcomb RD, Löfstedt C. A sex pheromone receptor in the hessian fly Mayetiola destructor (Diptera, Cecidomyiidae). Front Cell Neurosci. 2016;10:212.
Zhou J-J, Huang W, Zhang G-A, Pickett JA, Field LM. “Plus-C” odorant-binding protein genes in two Drosophila species and the malaria mosquito Anopheles gambiae. Gene. 2004;327:117–29.
Keeling CI, Yuen MMS, Liao NY, Docking TR, Chan SK, Taylor GA, et al. Draft genome of the mountain pine beetle, Dendroctonus ponderosae Hopkins, a major forest pest. Genome Biol. 2013;14:R27.
Robertson HM, Gadau J, Wanner KW. The insect chemoreceptor superfamily of the parasitoid jewel wasp Nasonia vitripennis. Insect Mol Biol. 2010;19:121–36.
Robertson HM, Wanner KW. The chemoreceptor superfamily in the honey bee, Apis mellifera: expansion of the odorant, but not gustatory, receptor family. Genome Res. 2006;16:1395–403.
Katoh K, Misawa K, Ki K, Miyata T. MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Res. 2002;30:3059–66.
Capella-Gutiérrez S, Silla-Martínez JM, Gabaldón T. trimAl: a tool for automated alignment trimming in large-scale phylogenetic analyses. Bioinformatics. 2009;25:1972–3.
Price MN, Dehal PS, Arkin AP. FastTree2 - approximately maximum-likelyhood trees for large alignments. PlosOne. 2010;5(3):e9490.
Guindon S, Dufayard J-F, Lefort V, Anisimova M, Hordijk W, Gascuel O. New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Syst Biol. 2010;59:307–21.
Lanfear R, Frandsen PB, Wright AM, Senfeld T, Calcott B. PartitionFinder 2: new methods for selecting partitioned models of evolution for molecular and morphological phylogenetic analyses. Mol Biol Evol. 2016;34:772–3.
Rambaut A. FigTree v1.4.0, a graphical viewer of phylogenetic trees. http://tree.bio.ed.ac.uk/software/figtree/. 2014.
Wang J, Gao P, Luo Y, Tao J. Characterization and expression profiling of odorant-binding proteins in Anoplophora glabripennis Motsch. Gene. 2019;693:25–36.
MNA acknowledges Christer Löfstedt for hosting during the project period. RFM acknowledges Adam Schwartz (UW Oshkosh) for his assistance in annotation and Erin Scully (USDA-ARS) for the access to the A. planipennis genome. This research was enabled in part by support provided by WestGrid (https://www.westgrid.ca) and Compute Canada (www.computecanada.ca).
MNA’s salary was funded by the Swedish research council FORMAS (grant numbers 217–2014-689 and 2018–01444) throughout the study. The funding body had no influence over study design, analysis and interpretation of the data, or in the writing of the manuscript.
Ethics approval and consent to participate
Consent for publication
The authors declare that they have no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Table S1. Amino acid sequences and annotation details of the chemosensory genes identified in the genomes of Anoplophora glabripennis, Agrilus planipennis, and Dendroctonus ponderosae. Information on genomic location, gene structure, and protein length is presented alongside annotation notes where relevant. (XLSX 38 kb)
Table S2. Gene name correspondence and revisions made to original models annotated from transcriptomes of Dendroctonus ponderosae and Agrilus planipennis. (XLSX 19 kb)
Figure S1. Left panel: Unrooted phylogeny of select Minus-C odorant binding proteins (OBPs) to indicate the evolutionary relationships of the four main exons of DponOBP4. Included are OBPs from Dendroctonus ponderosae (Dpon, red), Agrilus planipennis (Apla, blue), Anoplophora glabripennis (Agla, green), and Tribolium castaneum (Tcas, orange). The tree is based on a trimmed MAFFT alignment, and constructed using FastTree. Numbers at nodes are local support values, calculated using the Shimodaira-Hasegawa (SH) test implemented in FastTree. Exact SH values are only shown if > 0.7, whereas SH values for all branches are indicated by the colored circles; support increases with the size and brightness of the circles. The sources of sequence data and explanation of protein suffixes are detailed in the Materials and Methods section. Right panel: Amino acid identity matrix of the four major exons of DponOBP4, calculated using Geneious software based on a MAFFT alignment. (JPG 719 kb)
Translated amino acid sequences of all chemosensory genes annotated in the present study from Anoplophora glabripennis, Agrilus planipennis, and Dendroctonus ponderosae. (TXT 157 kb)
About this article
Cite this article
Andersson, M.N., Keeling, C.I. & Mitchell, R.F. Genomic content of chemosensory genes correlates with host range in wood-boring beetles (Dendroctonus ponderosae, Agrilus planipennis, and Anoplophora glabripennis). BMC Genomics 20, 690 (2019). https://doi.org/10.1186/s12864-019-6054-x
- Odorant receptor
- Ionotropic receptor
- Gustatory receptor
- Odorant binding protein
- Chemosensory protein
- Sensory neuron membrane protein
- Host specificity
- Pest insect