- Research article
- Open Access
The microbiota structure in the cecum of laying hens contributes to dissimilar H2S production
BMC Genomics volume 20, Article number: 770 (2019)
Host genotype plays a crucial role in microbial composition of laying hens, which may lead to dissimilar odor gas production. The objective of this study was to investigate the relationship among layer breed, microbial structure and odor production.
Thirty Hy-Line Gray and thirty Lohmann Pink laying hens were used in this study to determine the impact of cecal microbial structure on odor production of laying hens. The hens were managed under the same husbandry and dietary regimes. Results of in vivo experiments showed a lower hydrogen sulfide (H2S) production from Hy-Line hens and a lower concentration of soluble sulfide (S2−) but a higher concentration of butyrate in the cecal content of the Hy-Line hens compared to Lohmann Pink hens (P < 0.05), which was consistent with the in vitro experiments (P < 0.05). However, ammonia (NH3) production was not different between genotypes (P > 0.05). Significant microbial structural differences existed between the two breed groups. The relative abundance of some butyrate producers (including Butyricicoccus, Butyricimonas and Roseburia) and sulfate-reducing bacteria (including Mailhella and Lawsonia) were found to be significantly correlated with odor production and were shown to be different in the 16S rRNA and PCR data between two breed groups. Furthermore, some bacterial metabolism pathways associated with energy extraction and carbohydrate utilization (oxidative phosphorylation, pyruvate metabolism, energy metabolism, two component system and secretion system) were overrepresented in the Hy-Line hens, while several amino acid metabolism-associated pathways (amino acid related enzymes, arginine and proline metabolism, and alanine-aspartate and glutamate metabolism) were more prevalent in the Lohmann hens.
The results of this study suggest that genotype of laying hens influence cecal microbiota, which in turn modulates their odor production. Our study provides references for breeding and enteric manipulation for defined microbiota to reduce odor gas emission.
The poultry industry is a vital livestock sector producing meat and eggs for human consumption. The short digestive tract and fast chyme transit time (less than 2.5 h) in the upper intestine of chicken results in incomplete digestion and absorption of feed nutrients in the foregut [1, 2]. However, large quantities and diverse microorganisms inhabit in the cecum of laying hens, which prolongs the retention time of digesta to approximately 12–20 h. This latter process is known as microbial-based metabolism [3,4,5]. Previous studies have demonstrated that this “microbial organ” expands the biochemical potential in feed degradation, micronutrient production, odor emission, pathogen exclusion and immune system development in poultry [6,7,8].
It is well known that the ecological environment in the cecum of laying hens is composed of microorganisms and metabolites. Furthermore, it is generally accepted that the proportions and concentrations of these metabolites depend on the microbial composition, which to some degree, could be modulated by the composition and structure of the diet . The degradation of undigested feed components (mainly undigested carbohydrates and proteins) and uric acid produce various bacterial metabolites. The rapid hydrolysis of uric acid and urea by microbial enzymes leads to the formation of ammonium, which is subsequently converted into NH3 and discharged into the atmosphere. The microbiota in the cecum of laying hens also contributes to sulfur metabolism, and the dissimilatory sulfate-reduction activity by sulfate-reducing bacteria (SRB) is the primary source for H2S formation and emission [10, 11]. Furthermore, the fermentation of undigested protein also contributes to NH3 and H2S discharge, but this pathway is not the dominant way for odor production [12,13,14]. In contrast, the fermentation of undigested carbohydrates by acid-producing bacteria provides numerous volatile fatty acids (VFAs), such as acetate, propionate and butyrate . Previous studies found that an active carbohydrate fermentation (adequate production of VFAs) by gut acid-producing bacteria could result in the suppression of protein fermentation and partly inhibit the production of odor gas [16, 17].
At present, China has the largest number of laying hens in the world, with Lohmann and Hy-Line being the major breeds. Thus, there is increasing concern from the general public regarding odor emission from intensive poultry production. Long term exposure to odor gas reduces the productivity of animals and also induces diseases . NH3 comprises the largest proportion of odor gas in livestock and poultry facilities . In addition, H2S is also the predominant ones because the concentration of it only second to that of ammonia and have significantly lower odor thresholds than NH3 which also contributes to odor in the farm environment [20, 21]. Therefore, reducing NH3 and H2S which represent odor gas generated from the layer farm besides mitigating the environmental problems will also have a positive effect on the intestinal health of the animal. Nutritional manipulations, for example, the inclusion of probiotics or organic acids in the diet, have been used to regulate cecal microbiota for mitigating odor emission in animals . Several recent studies have explored the associations between intestinal microbiota and performance traits in chickens and found that the gut microbial structure influences feed conversion efficiency and body weight gain [2, 23,24,25,26,27,28]. However, limit studies have been conducted to explore the relationship between gut microbiota and odor emission in laying hens when taking breed into consideration. A deeper understanding of the impact and mechanisms of cecal microbiota influencing odor production in laying hens could contribute to the breeding and selection of environmentally friendly laying hens and the development of more effective strategies to optimize an intestinal microbiota structure that would achieve more efficient odor mitigation and productivity improvements.
Based on the available information, we postulated that the gut microbiota structure, which differs among breeds, influences enteric odor production in chickens. The present study quantified and characterized the odor production of two most widely reared laying hen breeds, namely, Hy-Line Gray and Lohmann Pink, and investigated the population structure of their microbiota using 16S rRNA sequencing and q-PCR technology.
Production performance and odor production
The body weight, ADFI, laying rate, average egg weight and feed-egg ratio of the hens were not significantly different between the two breeds, which eliminated the confounding effects on odor gas production these performance traits divergences might exert (P > 0.05) (Additional file 1: Table S3). The recovery rates of the 12 chambers were between 83.85 to 96.62% and 83.01 to 95.31% for NH3 and H2S respectively (Additional file 1: Table S4). Due to the good recovery rate, these 12 chambers could be used for odor measurement. The total daily H2S production per kg average daily feed intake of Hy-Line Gray was significantly lower than that of Lohmann Pink (7.75 vs. 4.17 mg/kg ADFI for Lohmann and Hy-Line, respectively) (P < 0.05) (Fig. 1a, b). However, no significant difference was observed in total daily NH3 production per kg average daily feed intake between two breeds (33.23 vs. 31.30 mg/kg ADFI) (P > 0.05) (Fig. 1c, d).
Profile characteristics in cecal content
The pH value and concentrations of ammonium, soluble sulfide (S2−), sulfate radical (SO42−) and VFAs in cecal content were determined. Compared to Lohmann, Hy-Line showed markedly lower concentrations of soluble sulfide (31.54 vs. 24.53 μg/g) but higher concentrations of butyrate (3.20 vs. 4.81mmoL/L) in the cecal content (Fig. 2a, b) (P < 0.05). However, the pH value and the concentrations of ammonium, sulfate radical, acetate, propionate and Total VFAs in cecal content were not different (P > 0.05) (Additional file 1: Table S5).
After quality processing, a total of 4,641,615 clean sequences were obtained from 60 cecal content samples with an average of 77,360 reads per sample. The sequences were further clustered into an average of 674 operational taxonomic units (OTUs). Good’s coverage was greater than 99.7%, indicating that most of the OTUs present in the samples were detected and the sampling depth was sufficient to characterize the microbiota in cecal content samples (Additional file 1: Figure S2).
Microbial diversity and relative abundance
The Chao1 and Observed species indices are indicators for species abundance, while the Shannon index is applied to evaluate the diversity of gut microbiota. Comparative analysis between breeds revealed that Hy-Line had a higher taxa abundance and diversity than Lohmann, as indicated by significantly different Chao1, Observed species and Shannon indices (P < 0.01) (Fig. 3a-c). Between-group diversity was assessed using nonmetric multidimensional scaling (NMDS) analysis, the bacterial community profiles clustered into two groups, highlighting significant differences between two breeds (Fig. 3d). Bacteroidetes (mean relative abundance: 49.9 and 48.4% for Lohmann and Hy-Line, respectively), Firmicutes (27.8 and 27.3%) and Fusobacteria (11.5 and 12.4%) were the most abundant bacteria phyla in the cecum of laying hens. Besides, Proteobacteria (4.9 and 4.7%), Euryarchaeota (1.6 and 0.9%), Melainabacteria (1.1 and 1.4%) and Unidentified Bacteria (1.4 and 3.3%) also had a high relative abundance (> 1%) (Fig. 4a). The proportion of Unidentified Bacteria but not the other six dominant phyla were significantly different between two breeds (Hy-Line> Lohmann, P = 0.007). At the genus level, nine genera were determined with > 1% relative abundance in both two breeds. Bacteroides (mean relative abundance: 31.0 and 28.0% for Lohmann and Hy-Line, respectively), Fusobacterium (11.5 and 12.4%) and Faecalibacterium (4.7 and 3.5%) were found to be most common in the two breeds (Fig. 4b). Furthermore, Helicobacter (1.2 and 3.1%), Megamonas (1.8 and 1.2%), Unidentified Lachnospiraceae (2.2 and 2.1%), Alistipes (2.8 and 1.5%), Sutterella (2.4 and 1.6%) and Unidentified Clostridiales (1.1 and 1.6%) also accounted for the predominant genera. Among them, the abundance of Helicobacter (Hy-Line>Lohmann, P = 0.003), Sutterella (Lohmann>Hy-Line, P = 0.008) and Alistipes (Lohmann>Hy-Line, P = 0.002) were significantly different between two breeds. In total, 42 genera showed significant differences between the two breeds but the abundance of most different genera is less than 1% (Additional file 1: Table S6). We focused on some bacteria which related to H2S and butyrate production and found that Mailhella (0.07 and 0.04%, P = 0.001) and Lawsonia (0.06 and 0.0004%, P = 0.003) were significantly more abundant in Lohmann, whereas the relative abundance of Butyricicoccus (0.32 and 0.48%, P = 0.035), Butyricimonas (0.06 and 0.18%, P = 0.001), and Roseburia (0.007 and 0.02%, P = 0.001) were significantly higher in Hy-Line (Fig. 5a-e). In next step, we conducted correlation analysis between odor production and the abundance of odor-related bacteria (Additional file 1: Table S7), and found that the abundance of Desulfovibrio, Mailhella, and Lawsonia had a significantly positive correlation with H2S production, whereas the abundance of Butyricicoccus, Butyricimonas and Roseburia had a significantly negative correlation with H2S production (P < 0.05). In addition, the abundance of Fusobacterium and Unidentified Clostridiales had a significantly positive correlation with NH3 production (P < 0.05).
The relative abundance of the aprA gene between breeds
In consideration of the limitation of 16S rRNA sequencing on quantifying the abundance of SRB. We conducted Real-time PCR to detect the relative abundance of specific functional aprA gene of SRB in cecal content. Compared to Hy-Line, Lohmann had a higher relative abundance of SRB functional aprA gene (2.32 vs. 1) (P < 0.05), indicating that the relative abundance of SRB in Lohmann are significantly higher than Hy-Line (Fig. 5f).
Predictive functions of the microbial community
Based on the PICRUSt functional prediction, 35 pathways were shown to achieve significant differences at KEGG level 3 (Fig. 6). Thirteen pathways were upregulated in the microbial community of Hy-Line whereas 22 pathways were upregulated in the microbial community of Lohmann. The majority of these pathways are involved in substance metabolism. We focused on some pathways which related to carbohydrate metabolism and protein metabolism. Notably, a significant elevation in oxidative phosphorylation, pyruvate metabolism, energy metabolism, two component system, secretion system, but a decline in amino acid related enzymes, arginine and proline metabolism, and alanine-aspartate and glutamate metabolism were observed in Hy-Line.
Odor production and profile characteristics in fermentation broth
We found that different H2S production between the two breeds may result from dissimilar cecal microbiota structure. Then we extracted the microbial flora from the cecum of laying hens and conducted in vitro fermentation trial to further verify the relationship between microbiota structure and H2S production. Similarly, lower H2S production (179.96 vs. 101.91 μg) was observed in the Hy-Line (P < 0.05) (Fig. 7a), but NH3 production (28.73 vs. 19.36 μg) did not differ between two groups (P > 0.05) (Fig. 7b). Lower concentrations of soluble sulfide (1.80 vs. 1.17 μg/mL) and but higher concentrations of acetate (13.24 vs. 18.21 mmoL/L), butyrate (1.79 vs. 2.66 mmoL/L) and total VFAs (21.54 vs. 28.69 mmoL/L) were observed in the Hy-Line fermentation broth (P < 0.05) (Fig. 7c, d). In addition, lower concentrations of sulfate radical and ammonium were also observed in the Hy-Line fermentation broth (P < 0.05), but the pH value and the concentrations of propionate in fermentation broth did not differ between two breeds (P > 0.05) (Additional file 1: Table S5).
Within host species, factors such as diet and feeding regime could affect the population structure of chicken microbiota . Investigating the role of breed in microbiota structure requires adequate control of confounding effects that may be caused by other factors. Thus, we selected only the two most commonly used laying hen breeds (Hy-Line and Lohmann) and strictly maintained them in the same location and with the same feeding regime. The comparison of microbiota composition between chicken breeds can provide valuable insight into the association between microbiota composition and odor emission.
Based on the principle of the respiration chambers for pigs that we previously constructed and used to measure enteric methane production [30,31,32], we constructed 12 respiration chambers for laying hens. In the present study, a lower H2S production and lower concentration of soluble sulfide in the cecal content were shown in Hy-Line hens compared to those of Lohmann hens (Figs. 1 and 2a), these findings were in agreement with a previous report that the concentrations of gut soluble sulfide and sulfate radical are positively correlated with the release of hydrogen sulfide . In addition, a higher production of butyrate in the Hy-Line cecal content indicated that active carbohydrate metabolism may exist in the Hy-Line cecum (Fig. 2b). However, odor production and cecal profiles can also be influenced by the nature of the substrates flowing out from the small intestine. Fermentation compounds from the distal ileum (into cecum) were probably different as a consequence of their different foregut digestibility between the two breeds. To better explain the role of cecal microbiota in the different odor production and cecal profiles, we conducted a follow-up in vitro fermentation experiment to exclude the possible confounding effects that may be caused by any differences in foregut digestibility between the two breeds. The results of the latter experiment are also in accordance with the higher production of H2S and soluble sulfide in Lohmann from the in vivo trial (Fig. 7a, b). Taken together, these results indicate that Hy-Line has a microbial structure that favors lower H2S production and soluble sulfide concentrations but higher VFA production. Interestingly, we did not find a significant difference in NH3 production and ammonium concentration (except for a lower concentration of ammonium in the Hy-Line incubation broth) in our in vivo and in vitro experiments. We speculate that there may be no notable differences in the abundance of some dominant NH3 producing bacteria between the two breeds.
To validate the hypothesis that differences in odor production and cecal profiles are associated with different microbiota structures and microbial metabolic activities between the two breeds, we further compiled the 16S rRNA gene amplicon sequencing data and used the computation tool PICRUSTs to predict microbial community functions. In agreement with previous studies [34,35,36], Bactemidetes, Firmicutes and Fusobacteria were the most predominant phyla in both breeds. In this study, we focused on the major differences in microbiota involved in H2S genesis and butyrate production. Most SRB belong to the family Desulfovibrionaceae which can use preliminary degraded organic substances as electron donors to reduce sulfate to produce H2S . Our 16S data only annotated four genera (Desulfovibrio, Mailhella, Bilophila and Lawsonia) belonging to family Desulfovibrionaceae. These H2S-related bacteria were found to be positively correlated with H2S production (Additional file 1: Table S7), and it showed a significantly higher abundance of Mailhella and Lawsonia in the cecum of Lohmann, although these two genera are quantitatively small in cecum (Fig. 5a, b). In consideration of the inadequate annotation information form 16S rRNA sequencing data due to the low abundance of SRB in the gut (approximately 0.028–0.097%) . We further quantified the functional gene aprA that encodes key enzymes of the dissimilatory sulfate-reduction, which is appropriate to determine the number of SRB in the gut . The relative quantification of SRB, to some extent, was consistent with the results of 16S rRNA sequencing (Fig. 5f). It can be inferred that a lower presence of SRB in the Hy-Line hens resulted in lower H2S production. Some of the identified differential genera, such as Butyricicoccus, Butyricimonas and Roseburia, are known butyrate producers . We found that these butyrate producers were negatively correlated with H2S production and were more abundant in the Hy-Line (Fig. 5c-e), which is in line with the fact that the dissimilatory sulfate-reduction of SRB will compete with butyrate-producing bacteria and that competitive inhibition of SRB exerted by these acid-producers can indirectly mitigate the production of H2S . Some genera, such as Fusobacterium (11.4 and 12.4%, P = 0.712), Unidentified Clostridiales (1.1 and 1.6%, P = 0.052) and Campylobacter (0.2 and 0.1%, P = 0.467), have strong urease activity and are considered to be typical NH3 producers [41, 42]. We demonstrated strong links between the abundance of these bacteria and NH3 production. However, no significant differences in the abundance of these bacteria explained the similar NH3 production between the two breeds. Based on our results, these bacteria may become important regulation targets for NH3 emission reduction in future research.
Based on the PICRUSt analysis, some pathways associated with energy extraction and provision (including oxidative phosphorylation, pyruvate metabolism, energy metabolism), bacterial colonization and proliferation (including two component system and secretion system) were overrepresented in Hy-Line (Fig. 6). These pathways are crucial for substrate sensing and foraging, as well as carbohydrate substrate transport and utilization , implying that a more active carbohydrate metabolism existed in the Hy-Line cecum. In contrast, the majority of amino acid metabolism-associated pathways (including amino acid related enzymes, arginine and proline metabolism, and alanine-aspartate and glutamate metabolism but not cysteine and methionine metabolism) were suppressed in Hy-Line. Active carbohydrate fermentation could inhibit the fermentation of protein, but suppressed protein metabolism in Hy-Line did not lead to lower NH3 production. The reason for this discrepancy may be that the production of NH3 mainly depends on the hydrolysis of uric acid instead of the fermentation of protein [16, 43], thus a nonsignificant difference in NH3 producing bacteria between the two breeds resulted in similar NH3 production. In addition, due to the limitation of 16S rRNA for functional prediction, we did not annotate and attain pathways that were directly related to bacterial sulfur metabolism. The state of microbiota and relationships with their colonized ecosystem could be more accurately examined using deep-sequencing technologies such as the metatranscriptome and metaproteome.
In this study, we found that Hy-Line hens exhibited lower H2S production as a result of a lower abundance of SRB but a higher abundance of butyrate producers in the cecum compared to Lohmann hens. Our study suggested that, at least in part, different microbiota structures in the cecum of laying hens contributes to dissimilar odor production, which offers a reference for targeted odor mitigation by the enteric manipulation of identified odor-related microbiota.
Experiment 1: in vivo trial
Animals and feeding
Approximately 100 Hy-Line Gray and 100 Lohmann Pink chicks were hatched and fed together in a commercial hatchery (Peng Chang Co., Ltd., Shenzhen, China). Upon reaching 28 weeks of age, 30 laying hens from each breed with similar weights (1.70 ± 0.02 and 1.71 ± 0.02, respectively for Hy-Line and Lohmann) were selected and moved to twelve 2 × 1 × 1.2 m respiration chambers with five hens per chamber (replicates) which were placed in an environmentally controlled room for a 21-day experiment. The hens were all fed the same commercial laying hen diet ad libitum (Additional file 1: Table S1). Clean drinking water was also made available at all time to the hens. The hens were kept in a room maintained at 24 °C with a 12 h lighting and 12 h dark management schedule. The 21-day experiment consisted of 18 days of feeding and 3 days of gas collection. Body weight, average daily feed intake (ADFI), laying rate, average egg weight and feed-egg ratio of the hens were recorded and calculated during the 21-day experimental period. It is worth noting that the diet, age, weight, and feeding environment are consistent between two breeds after hatching and during the experimental period in order to minimize the confounding effects these factors might cause.
Design of the respiration chambers and gas collection
Twelve respiration chambers were constructed (patent number: 201610091580.9) (Additional file 1: Figure S1). Each chamber was 2.0 m long× 1.0 m wide× 1.2 m high and constructed using a metal frame covered with transparent poly methyl methacrylate sheets except for the bottom which was made of a steel sheet. Each chamber was fitted with two doors in the front: one for feeding, egg collection and placing and removing the hens and the other for feces removal. Drinking water was provided using a drinker nipple located at the front of the chamber. The respiration chamber was ventilated using an electrical pump (Type: ACO-009D, Hailea, Guangzhou, China) at the outlet end. The ventilation rate was maintained at 40 L/min using a gas flow-meter (Type: LZB-2WB, Specifications: 6–60 L/min, ZhenXing Flowtech, Yuyao, China). The end of the gas outlet was connected to three sampling tubes. The first tube was connected to a bottle of diluted sulfuric acid solution (50 mL of 0.05 mol/L per bottle) for absorption of NH3 in the outlet air which flows at 1 L/min flow (Type: LZB-4WB, Specifications: 0.6–6 L/min, ZhenXing Flowtech, Yuyao, China) (Additional file 1: Figure S1, 16). The second tube was connected to a bottle of cadmium sulfate solution (4.3 g 3CdSO4·8H2O and 0.3 g NaOH and 10 g ammonium alcohol polyvinyl phosphate were diluted to 1 L with water, 10 mL per bottle) for an H2S absorption (flow rate of 1 L/min) (Additional file 1: Figure S1, 18). The third tube served to exhaust the remaining outlet air (Additional file 1: Figure S1, 17). The daily gas collection lasted for 24 h with a short intermittent break (approximately 2 min) every 2 h to remove and replace the respective gas absorption solutions. The above procedure was repeated over 3 days (as 3 replicates for the gases produced).
Recovery rate of the respiration chambers
The recovery rate of the system was determined based on the procedure of Ji et al. . Standard gas was released into the chamber using a precision reducer and flow meter to ensure a uniform release rate. The standard gas for NH3 comprised 1.6% NH3 (v/v) and 98.4% (v/v) nitrogen (N2). The standard gas for H2S comprised 0.02% H2S (v/v) and 99.98% (v/v) N2. The respiration chamber was ventilated, and samples of outlet air were collected at regular intervals (2 h) as described above. The recovery rate of system (R) was calculated as follows:
where Mrec is the mass of gas recovered by gas collection measurement (μg); Mrel is the mass of gas released (μg); Mgas bottle is the mass of gas released by gas bottle (μg); and Mgas air is the mass of gas in the inlet air (μg).
Measurement of odor production
Gas production (μg) from the chickens every 2 h was calculated as:
where M is the mass of gas produced by chickens in 2 h (μg); Ms is the mass of gas in the absorption solution (μg); Ca is the concentration of gas in the inlet air (μg/L), Va is the volume of inlet air. The ventilation rate (40 L/min) multiplied by 120 min yields the volume of inlet air and was corrected to standard temperature and pressure. R is the recovery rate of gas of the system.
The mass of NH3 and H2S in solution was measured with a spectrophotometer (Ao Yi technology Co. Ltd., Shanghai, China) based on the Chinese National Environmental Protection Strands (determination of ammonia, Nessler’s reagent spectrophotometry; standard method for hygienic examination of hydrogen sulfide in air of residential areas, methylene-blue spectrophotometric method).
Cecal sample collection
At the end of the experiment, all birds were euthanized by cervical dislocation, and then the cecum was ligated and removed from the carcass. The content was aseptically collected into an Eppendorf tube and immediately put in liquid nitrogen and stored at − 80 °C for further experiments.
Measurement of cecal profiles
Accurately quantified 1.0 g of cecal content was mixed adequately with 9 mL deionized water in a 15 mL centrifuge tube and then centrifuged at 10,000×g for 5 min, the supernatant was used for measurement. The pH value was determined using a pH meter (INESA Scientific Instrument Co., Ltd., Shanghai, China). The ammonium-nitrogen was determined with a spectrophotometer (Ao Yi technology Co., Ltd., Shanghai, China) based on Nessler’s reagent spectrophotometry method. The sulfate radical (SO42−) concentrations were determined using a turbidimetric method according to Deng et al. . The soluble sulfide (S2−) concentrations were determined using a methylene-blue colorimetric method. The concentrations of acetate, propionate and butyrate were determined using high-performance liquid chromatography (Agilent Technologies, Inc., California, USA) with an Agilent SB-C18 column (150 × 4.6 mm). Samples were eluted with methanol mixed sodium dihydrogen phosphate buffer (28:72, v/v) at 1 mL/min and were detected with a U.V. monitor at a wavelength of 213 nm.
Bacterial DNA extraction, sequence processing and data analysis
Approximately 200 mg of cecal content was used for total DNA extraction using a QIAamp PowerFecal DNA Kit (QIAGEN, Germany) following the manufacturer’s instructions. DNA quantity and quality were measured on a Qubit 2.0 fluorometer (Thermo Fisher scientific, MA, US) and gel electrophoresis. The V3-V4 hypervariable regions of 16S rRNA genes were amplified using primers (forward: 5′-CCTACGGGNGGCWGCAG-3′ and reverse: 5′-GACTACHVGGGTATCTAATCC-3′) . PCR amplifications were carried out using 25 μL reaction mixtures containing 12.5 μL 2X KAPA HiFi HotStart ReadyMix (Kapa Biosystems, UK), 5 μL forward and reverse primers and 2.5 μL DNA template. Amplicons were performed using the following conditions: denaturation at 94 °C for 5 min, 30 cycles of amplification including denaturation (94 °C, 30 s), annealing (50 °C, 30 s), and elongation (72 °C, 30 s), and final elongation at 72 °C for 10 min. The final products were sequenced using an Illumina MiSeq platform at Novogene Co. Ltd. (Tianjin, China).
Raw reads were uploaded into QIIME software v2.1 and quality trimmed. Reads containing ambiguous bases, a low quality score (a quality score lower than 25), or reads shorter than 200 bp were discarded. Trimmed sequences were clustered into 97% similarity OTUs. Annotation assignment and richness analysis of reference OTU sequences were performed by mapping against the Greengenes database with a 0.5 confidence threshold and were taxonomically identified to the genus level. Analysis of alpha diversity (Observed species, Chao 1 index and Shannon index) as well as Good’s Coverage estimates were calculated in QIIME. For beta diversity analysis, NMDS was used to assess the differences in microbiota structure. Comparative analysis for taxa was performed where the Benjamini-Hochberg false discovery rate (FDR) was used for multiple test corrections to minimize FDR during group comparative analyses, and P-values less than 0.05 were considered statistically significant. Predictive functions were characterized using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) based on the Kyoto Encyclopedia of Genes and Genomes database (KEGG) .
Q-PCR of the functional gene (adenosine-5′-phosphosulfate reductase alpha subunit gene, aprA) of SRB
We further determined the relative abundance of SRB between the two breed groups through their function marker gene aprA using q-PCR with the 16S rRNA gene as the reference gene [46, 47] (Additional file 1: Table S2). The total DNA of the bacteria was extracted as described above. The reaction mixtures contained 10 μL SYBR® Green PCR Master Mix (QIAGEN, Germany), 0.5 μL of each primer, 1 μL of DNA samples and 8 μL H2O. The q-PCR cycle was set as follows: initial denaturation for 2 min at 95 °C, followed by 35 cycles of denaturing for 15 s at 95 °C, annealing for 30 s at 60 °C, and elongating for 25 s at 72 °C. The relative abundance of the aprA gene was compared using the 2-∆∆CT value.
Experiment 2: in vitro fermentation trial
To better explain the impact of cecal microbiota on the differences in H2S production, a follow-up in vitro fermentation study was conducted with 2 inocula × 6 replicates (12 syringes) plus two blanks, making a total of 14 syringes.
Preparation of the inoculum and fermentative substrate
Twenty Hy-Line Gray and twenty Lohmann Pink laying hens at 28 weeks of age were sacrificed, and the cecum was ligated immediately. The cecal contents in the same breed group were pooled, thoroughly mixed and diluted in sodium and ammonia bicarbonate buffer solution (35.0 g NaHCO3 + 4.0 g NH4HCO3 for a 1 L volume) at a ratio of 1:3 (W/V) according to the method of Wang et al. . The intestinal content–buffer mixture was stirred for 60 s in a blender after which the solution was squeezed through four layers of surgical gauze, and then mixed with the buffer mineral solution at a 1:2 ratio (V/V) at 40 °C under continuous flushing with CO2. A corn-soybean basal layer diet was used as a substrate for the fermentation.
In vitro fermentation
Approximately 30 mL of the inoculum was added to each 100-mL gas syringe with 0.5 g of the substrate. After removing the air from the head-space of the syringe, the syringe was sealed with a clip and placed in a 40 °C incubator rotated at 60 rpm for 12 h. The volume of gas produced at the head-space was recorded. At the end of incubation, the syringes were put into an ice box to stop further fermentation, and the gas was transferred by syringes into gas collection bag for NH3 and H2S analysis. Ten milliliter fermentation broth was sampled and stored at − 80 °C for chemical analysis. The quantity of NH3 in the syringes and the concentrations of ammonium-nitrogen in fermentation broth were determined using Nessler’s reagent spectrophotometry method. The quantity of H2S in the syringes and the concentrations of soluble sulfide (S2−) in the fermentation broth were determined using the methylene-blue colorimetric method. The pH value was determined using a pH meter (INESA Scientific Instrument Co., Ltd., Shanghai, China). The concentration of sulfate radical (SO42−) was determined using the turbidimetric method. The concentrations of VFAs were determined using high-performance liquid chromatography as described in Experiment 1(“Measurement of cecal profiles section”).
The significance of the data was analyzed with an independent-samples t-test using the commercially available statistical software package SPSS 22.0 (SPSS, Chicago, IL, USA). Differences were considered significant if P < 0.05. Data are presented as the means with their standard errors. The correlation analysis was conducted with Pearson correlation-based model using SPSS 22.0.
Availability of data and materials
The 16S rRNA datasets generated and analysed during the current study are available in the European Nucleotide Archive database under the accession number PRJEB30541.
Average daily feed intake
- NH3 :
- S2− :
- SO4 2− :
Volatile fatty acids
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This work was supported by a grant from Natural Science Foundation of China (No.31872993) and the earmarked fund from Modern Agroindustry Technology Research System (CARS-41). The funders had no role in the design, analysis, or interpretation of these experiments.
Ethics approval and consent to participate
The protocol for this experiment was approved by the Animal Experimental Committee of South China Agricultural University (SYXK2014–0136).
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The authors declare that they have no competing interests.
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Table S1. Diet composition and nutrient levels. Table S2. The primer sequences of the aprA gene and 16S rRNA gene. Table S3. The production performance of laying hens. Table S4. The recovery of respiration chamber system. Table S5. Profile characteristics in cecal content and fermentation broth. Table S6. The relative abundance of the 42 significantly different genera between two groups. Table S7. Correlation coefficients of some odor-related microbiota and odor production. Figure S1. The structure of respiration chamber system. a The physical picture of respiration chamber. b Absorption bottle for gas collection. c The sketch of respiration chamber. 1 The inner cage, 2 Feed trough, 3 Inner floor, 4 Eggs trough, 5 Drinker, 6 fan, 7 PVC floor, 8 Organic glass cover, 9 Baffle of feeding, 10 Feces collection, 11 Baffle of feces collection, 12 Air inlet, 13 Air outlet, 14 Total air outlet, 15 Air pump, 16 Ammonia absorption bottle, 17 Exhaust hose, 18 Hydrogen sulfide absorption bottle, 19 Flowmeter. Figure S2. The Good’s coverage index box plots of two groups. Good’s coverage index with a box plot exhibiting the depth of 16S rRNA sequencing. Hn: Hy-Line, Ln: Lohmann.
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Huang, CB., Xiao, L., Xing, SC. et al. The microbiota structure in the cecum of laying hens contributes to dissimilar H2S production. BMC Genomics 20, 770 (2019). https://doi.org/10.1186/s12864-019-6115-1
- Laying hen