Table 2 List of primers used for Real-Time quantitative RT-PCR
S. No | Gene name | Forward primer (5`-3`) | Reverse primer (5`-3`) |
|---|---|---|---|
1 | Photosystem II protein PsbR | AGCTCCCACCTCAAGGAGAT | AGCAACTCTTCAGCCTCTGC |
2 | Photosystem I reaction centre subunit VI | AGGTGGAGGTTGCTGTGACT | CTTCTCTGCGACCGTTAAGC |
3 | Plant lipid transfer protein/seed storage | CAACAGCTGAGAGAACCCATC | GGCAGCTATGCCTTTCATCT |
4 | Ferredoxin-type protein | AAGGAGCTGGTTGTCAAGGA | ATCTGCTCACACATCGCAAG |
5 | Fructose-bisphosphate aldolase, class-I | CCATGATGTGGTGGACGATA | GGCTAGCCTGCGATGTCTAC |
6 | Ubiquitin-conjugating enzyme, E2 | AGGCTTCCGTGCTACACAAC | TTAAGGCAGGTTGCTCCTTC |
7 | Plant metallothionein, family 15 | GTTAGAACCTGGGTGGCATC | GATCTTTGGCTCGACTTGCT |
8 | Oxoglutarate/iron-dependent oxygenase | CCAGTCAAAGACTCGGAACC | GAAGGAGTCACCGTCTCCAG |
9 | Glutamine synthetase/guanido kinase, catalytic domain | CCCACTTTAGAGCGAGAGACTG | GTGAGATGACGGCGATGAC |
10 | Urease accessory protein UreD | CTCCAAGTTTCCGAGGATTG | CCCTAAGCCAGCACTGTAGC |
11 | 26 S rRNA | CCCTGTGGTAACTTTTCTG | GCTCGTTTGATTCTGATTTC |