Fig. 5

Linkage analysis for spotting mutation candidate genes. Genomic DNA from BW and PO control animals, along with a selection of S/+ F₁ animals, was PCR amplified for a set of candidate genes (Edn3, Ednrb, Kit, Kitl, Mitf, Pax3, Ret, Snai2, and Sox10), known to cause pigmentation defects in Mus and humans. Each amplicon contained a restriction enzyme recognition site polymorphism between BW and PO (see Table 5). PCR amplicons were digested with the appropriate restriction enzyme and analyzed by agarose gel electrophoresis. Sox10 is the only candidate gene were all S/+ N₂ animals have both a PO and BW allele, demonstrating linkage with the Dominant spot phenotype. Each image was cropped to remove excessive space